Abstract
Seventy-one cyanobacteria containing cultures were enriched from various soil and water locations either under aerobic and/or anaerobic conditions on agar medium selective for nitrogen fixation. Kept under argon containing 1% CO2 for 24 and 48 h most of these cultures evolved hydrogen at very variable rates up to 116 μl per mg chlorophyll and hour as a mean value over a time period of 24h. Several samples evolved hydrogen more efficiently compared with known hydrogen producing pure strains from culture collections. Thirty-one of the investigated cultures showed a hydrogen formation higher than 10 μl per mg chlorophyll and hour measured over 24 or 48 h. Among these all the morphological forms of cyanobacteria i.e. unicellular and filamentous with or without heterocysts are found. Hence, selecting for nitrogen fixing cyanobacteria seems to be a practical method to find efficient hydrogen producers.
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References
Benemann, J. R., Weare, N. M.: Hydrogen evolution by nitrogen-fixing Anabaena cylindrica cultures. Science 184, 174–175 (1974)
Bothe, H., Loos, E.: Effect of far red light and inhibitors on nitrogen fixation and photosynthesis in the blue-green alga Anabaena cylindrica. Arch. Microbiol. 86, 241–254 (1972)
Bothe, H., Tennigkeit, J., Eisbrenner, G.: The utilization of molecular hydrogen by the blue-green alga Anabaena cylindrica. Arch. Microbiol. 114, 43–49 (1977b)
Bothe, H., Tennigkeit, J., Eisbrenner, G.: The hydrogenase-nitrogenase relationship in the bluegreen alga Anabaena cylindrica. Planta 133, 237–242 (1977a)
Daday, A., Platz, R. A., Smith, G. D.: Anaerobic and aerobic hydrogen gas formation by the blue-green alga Anabaena cylindrica. Appl. and Environ. Microbiol. 34, 478–483 (1977)
Eisbrenner, G., Distler, E., Floener, L., Bothe, H.: The occurrence of the hydrogenase in some blue-green algae. Arch. Microbiol. 118, 177–184 (1978)
Fujita, Y., Ohoma, H., Hattori, A.: Hydrogenase activity of a cell free preparation obtained from the blue-green alga Anabaena cylindrica. Plant Cell Physiol 5, 305–314 (1964)
Gest, H., Ormerod, J. G., Ormerod, K. S.: Photometabolism of Rhodospirillum rubrum: Light-dependent dissimilation of organic compounds to carbon dioxide and molecular hydrogen by an anaerobic citric acid cycle. Arch. Biochem. Biophys. 97, 21–33 (1962)
Hallenbeck, P. C., Benemann, J. R.: Characterisation and partial purification of the reversible hydrogenase of Anabaena cylindrica. FEBS Letters 94, 261–264 (1978)
Jeffries, T. W., Timouran, H., Ward, R. L.: Hydrogen production by Anabaena cylindrica: Effects of varying ammonium and ferric ions, pH and light. Appl. Environ. Microbiol. 35, 704–710 (1978)
Jones, L. W., Bishop, N. I.: Simultaneous measurement of oxygen and hydrogen exchange from the blue-green alga Anabaena. Plant Physiol. 57, 659–665 (1976)
Kenyon, C. N., Rippka, R., Stanier, R. Y.: Fatty acid composition and physiological properties of some filamentous blue-green algae. Arch. Mikrobiol. 83, 216–236 (1972)
Lambert, G. R., Smith, G. D.: Hydrogen formation by marine blue-green algae. FEBS Letters 83, 159–162 (1977)
MacKinney, G.: Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–322 (1941)
Mitsui, A., Kumazawa, S.: Hydrogen production by marine photosynthetic organisms as a potential energy resource. In: Biological solar energy conversion (A. Mitsui, S. Miyachi, A. San Pietro, S. Tamura, (eds.), pp. 23–49. New York, San Franzisco, London: Academic Press 1977
Neil, G., Nicholas, D. J. D., Bockris, J. O. M., McCann, J. F.: Photosynthetic production of hydrogen. Int. J. Hyd. Energy 1, 45–48 (1976)
Ormerod, J. G., Ormerod, K. S., Gest, H.: Light-dependent utilisation of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; Relationships with nitrogen metabolism. Arch. Biochem. Biophys. 94, 449–463 (1961)
Peters, G. A., Evans, W. R., Toia, R. E.: Azolla-Anabaena Azollae relationship, IV. photosynthetically driven, nitrogenase-catalyzed H2 production. Plant Physiol. 58, 119–126 (1976)
Peterson, R. B., Wolk, C. P.: Localization of an uptake hydrogenase in Anabaena. Plant Physiol. 61, 688–691 (1978)
Radway, J., Rosner, D., Greenbaum, J., Haynes, L.: Hydrogen producing tropical marine photosynthetic microorganisms — isolation and morphology. Plant Physiol. 59, 20 (1977)
Rippka, R., Waterbury, J. B.: Synthesis of nitrogenase by nonheterocystous cyanobacteria. FEMS Microbiol. Letters 2, 83–86 (1977)
Stewart, W. D. P., Lex, M.: Nitrogenase activity in the blue green alga Plectonema boryanum strain 594. Arch. Microbiol. 73, 250–260 (1970)
Stewart, W. P. D.: Biological and ecological aspects of nitrogen fixation by free-living micro-organisms. Proc. Roy. Soc. B 172, 267–388 (1969)
Tel-Or, E., Luijk, L. W., Packer, L.: An inducible hydrogenase in cyanobacteria enhances N2 fixation. FEBS Letters 78, 49–52 (1977)
Tel-Or, E., Luijk, L. W., Packer, L.: Hydrogenase in N2-fixing cyanobacteria. Arch. Biochem. Biophys. 185, 185–194 (1978)
De Vasconcelos, L., Fay, P.: Nitrogen metabolism and ultrastructure in Anabaena cylindrica. I. The effect of nitrogen starvation. Arch. Microbiol. 96, 271–280 (1974)
Weissman, J. C., Benemann, J. R.: Hydrogen production by nitrogen-starved cultures of Anabaena cylindrica. Appl. Environ. Microbiol. 33, 123–131 (1977)
Wyatt, J. T., Silvey, J. K. G.: Nitrogen fixation by Gloecapsa. Science 165, 908–909 (1969)
Zürrer, H., Bachofen, R.: Hydrogen production by the photosynthetic bacterium Rhodospirillum rubrum. Appl. Environ. Microbiol. 37, 783–793 (1979)
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Berchtold, M., Bachofen, R. Hydrogen formation by cyanobacteria cultures selected for nitrogen fixation. Arch. Microbiol. 123, 227–232 (1979). https://doi.org/10.1007/BF00406654
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DOI: https://doi.org/10.1007/BF00406654