Abstract
Acetobacterium woodii is a Gram-positive anaerobic nonsporeforming bacterium able to grow on H2 and CO2 as sole sources of energy. The product of fermentation is acetic acid. Fine structural analysis showed rod-shaped flagellated cells, and coccoid cells without flagella arranged predominantly in pairs and chains. The cell wall was found to be composed of three layers. The cell surface exhibited a periodic array of particles consisting of subunits. The cytoplasmic membrane showed particles either either in random distribution or in a hexagonal pattern. Intracytoplasmic membranes were rarely observed, whereas inclusion bodies of varying shapes, predominantly in an uncommon disc-shape, could frequently be observed. Their content was dissolved in ultrathin sections indicating hydrophobic nature.
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Balch, W. E., Schoberth, S., Tanner, R. S., Wolfe, R. S.: Acetobacterium, a new genus of hydrogen-oxidizing carbon dioxidereducing anearobes, and characterization of Acetobacterium woodii sp. nov. Int. J. Syst. Bacteriol. (1977, in press)
Bayer, M. E., Remsen, C. C.: Structure of Escherichia coli after freeze-etching. J. Bacteriol. 101, 304–313 (1970)
Beuscher, N., Mayer, F., Gottschalk, G.: Citrate lyase from Rhodopseudomonas gelatinosa: Purification, electron microscopy and subunit structure. Arch. Microbiol. 100, 307–328 (1974)
Frasca, J. M., Parks, V. R.: A routine technique for doublestaining ultrathin sections using uranyl and lead salts. J. Cell. Biol. 25, 157–161 (1965)
Glauert, A. M., Thornley, M. J.: The topography of the bacterial gell wall. Ann. Rev. Microbiol. 23, 159–198 (1969)
Hollaus, F., Sleytr, U.: On the taxonomy and fine structure of some hyperthermophilic saccharolytic Clostridia. Arch. Mikrobiol. 86, 129–146 (1972)
Langenberg, K. F., Bryant, M. P., Wolfe, R. S.: Hydrogen-oxidizing methane bacteria. II. Electron microscopy. J. Bacteriol. 95, 1124–1129 (1968)
Moor, H.: Use of freeze-etching in the study of biological ultrastructure. Int. Rev. Exp. Pathol. 5, 179–216 (1966)
Moor, H., Mühlethaler, K.: Fine structure in frozen-etched yeast cells. J. Cell Biol. 17, 609–628 (1963)
Mühlethaler, K., Moor, H., Szarkowski, J. W.: The ultrastructure of chloroplast lamellae. Planta (Berl.) 67, 305–323 (1965)
Schoberth, S.: Acetic acid from H2 and CO2: formation of acetate by cell extracts of Acetobacterium woodii. Arch. Microbiol. 114, 143–148 (1977)
Schoberth, S., Balch, W. E.: A new hydrogen-oxidizing acetateforming anaerobe. Am. Soc. Microbiol., Abstr. Ann. meeting, I 190 (1975)
Sleytr, U.: Fracture faces in intact cells and protoplasts of Bacillus stearothermophilus. A study by conventional freeze-etching and freeze-etching of corresponding fracture moieties. Protoplasma (Wien) 71, 295–312 (1970)
Sleytr, U. B.: Sell-assembly of the hexagonally and tetragonally arranged subunits of bacterial surface layers and their reattachment to cell walls. J. Ultrastruct. Res. 55, 360–377 (1976)
Smith, P. H., Hungate, R. E.: Isolation and characterization of Methanobacterium ruminantium n. sp. J. Bacteriol. 75, 713–718 (1958)
Spurr, A. R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969)
Valentine, R. C., Shapiro, B. M., Stadtman, E. R.: Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from E. coli. Biochemistry 7, 2143–2152 (1968)
Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell. Biol. 25, 407–408 (1965)
Weinstein, R. S., Koo, V. M.: Penetration of red cell membranes by some membrane-associated particles. Proc. Soc. Exp. Biol. Med. 128, 353–357 (1968)
Wolfe, R. S.: Microbial formation of methane. Adv. Microbiol. Physiol. 8, 107–146 (1971)
Wolin, E. A., Wolin, M. J., Wolfe, R. S.: Formation of methane by bacterial extracts. J. Biol. Chem. 238, 2882–2886 (1963)
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Mayer, F., Lurz, R. & Schoberth, S. Electron microscopic investigation of the hydrogen-oxidizing acetate-forming anaerobic bacterium Acetobacterium woodii . Arch. Microbiol. 115, 207–213 (1977). https://doi.org/10.1007/BF00406376
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DOI: https://doi.org/10.1007/BF00406376