Abstract
The objectives of this research were to determine if genes controlling the reaction to the herbicide acetochlor in maize (Zea mays L.) are active during both the haploid and the diploid phases of the life cycle and if pollen selection can be utilized for improving sporophytic resistance. Pollen of eight inbred lines, previously characterized through sporophytic analysis for the level of tolerance to acetochlor, showed a differential reaction to the herbicide forin vitro tube length; moreover, such pollen reactions proved to be significantly correlated (r =0.786*,df=6) with those of the sporophytes producing the pollen. Pollen analysis of two inbred lines (i.e. Mo17, tolerant, and B79, susceptible) and their single cross showed that thein vitro pollen-tube length reaction of the hybrid was intermediate between those of two parents. An experiment on pollen selection was then performed by growing tassels of Mo17xB79 in the presence of the herbicide. Pollen obtained from treated tassels showed a greater tolerance to acetochlor, assessed asin vitro tube length reaction, than pollen obtained from control tassels. Moreover, the backcross [B79 (Mo17xB79)] sporophytic population obtained using pollen from the treated tassels was more tolerant (as indicated by the fresh weight of plants grown in the presence of the herbicide) than was the control backcross population. The two populations did not differ when grown without the herbicide. These findings indicate that genes controlling the reaction to acetochlor in maize have haplodiploid expression; consequently, pollen selection can be applied for improving plant tolerance.
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References
Bino RJ, Franken J, Witsenboer HMA, Hill J, Dons JJM (1988) Effects ofAlternaria alternata F. sp.Lycopersici toxins on pollen. Theor Appl Genet 76:204–208
Breaux EJ (1986) Identification of the initial metabolites of Acetochlor in corn and soybean seedlings. J Agric Food Chem 34: 884–888
Cheng DSK, Freeling M (1976) Methods of maize pollen germination in vitro, collection, storage, and treatment with toxic chemicals; recovery of resistant mutants. Maize Genet Coop Newsl 50:11–13
Cottingam CK, Hatzios KK (1992) Basis of differential tolerance of two corn hybrids (Zea mays) to Metolachlor. Weed Sci 40: 359–363
Edwards R, Owen JW (1986) Comparison of glutathione S-transfrases ofZea mays responsible for herbicide detoxification in plants and suspension-cultured cells. Planta 169:208–215
Frascaroli E, Landi P, Sari Gorla M, Ottaviano E (1992) Variability of pollen and plant responses to glyphosate in maize. J Genet and Breed 46:49–56
Gcronwald JW (1989) Influence of herbicide safeners on herbicide metabolism: IV. Metabolism of chloroacetanilide herbicide in plants. In: Hatzios KK, Hoagland RE (eds) Crop safeners for herbicides, development, uses, and mechanisms of action. Academic Press, New York, 111–113
Hatzios KK (1989) Mechanisms of action of herbicide safeners: an overview: III. Mechanisms of safener action: physiological and biochemical aspects. In: Hatzios KK, Hoagland RE (eds) Crop safeners for herbicides, development, uses, and mechanisms of action. Academic Press, New York, 70–89
Hodgkin T (1990) In vitro pollen selection inBrassica napus L. for resistance to phytotoxic compounds fromAlternaria brassicicola (Schw.) Wilts. Sex Plant Reprod 3:116–120
Jaworski EF (1969) Analysis of the mode of action of herbicidal α-chloroacetamides. J Agric Food Chem 17:165–170
Landi P, Frascaroli E, Catizone P (1990) Variation and inheritance of reaction to acetochlor among maize inbred lines and hybrids. Euphytica 45:131–137
Laughnan JR, Gabay SJ (1973) Reaction of germinating maize pollen toHelminthosporium maydis pathotoxins. Crop Sci 13: 681–684
Leavitt JRC, Penner D (1978) Protection of corn (Zea mays L.) from Acetanilide herbicidal injury with the antidote R-25788. Weed Sci 26:653–659
Mozer TJ, Tiemeier DC, Jaworsky EG (1983) Purification and characterization of corn glutathione S-transferase. Biochemistry 22: 1068–1072
Mulcahy DL, Mulcahy GB (1975) The influence of gametophytic competition on sporophytic quality inDianthus chinensis. Theor Appl Genet 46:277–280
Obrigawitch T, Abernathy JR, Gipson JR (1980) Response of tellow (Cyperus esculentus) and purple (Cyperus rotundus) nutsedge to metolachlor. Weed Sci 28:708–715
Ottaviano E, Mulcahy DL (1989) Genetics of angiosperm pollen. Adv Genet 26:1–64
Pfahler PL (1983) Comparative effectiveness of pollen genotype selection in higher plants. In: Mulcahy DL, Ottaviano E (ed) Pollen: biology and implications for plant breeding. Elsevier, New York Amsterdam Oxford, 361–367
Polowick PL, Greyson RI (1982) Anther development, meiosis and pollen formation inZea tassels cultured in defined liquid medium. Plant Sci Lett 26:139–145
Richter J, Powles SB (1993) Pollen expression of herbicide target site resistance genes in annual ryegrass (Lolium rigidum). Plant Physiol 102:1037–1041
Sacher RF, Mulcahy DL, Staples RC (1983) Developmental selection during self pollination ofLycopersicon ×Solanum F1 for salt tolerance of F2. In: Mulcahy DL, Ottaviano E (eds) Pollen: biology and its implication for plant breeding. Elsevier, New York Amsterdam Oxford, 329–334
Sari Gorla M, Ottaviano E, Frascaroli E, Landi P (1989) Herbicidetolerant corn by pollen selection. Sex Plant Reprod 2:65–69
Sari Gorla M, Ferrario S, Rossini L, Frova C, Villa M (1993) Developmental expression of glutathione-S-transferase in maize and its possible connection with herbicide tolerance. Euphytica 67:221–230
Sari Gorla M, Ferrario S, Frascaroli E, Frova C, Landi P, Villa M, (1994) Sporophytic response to pollen selection for Alachlor tolerance in maize. Theor Appl Genet (in press).
Searcy KB, Mulcahy DL (1985a) Pollen selection and the gametophytic expression of metal tolerance inSilene dioica (Cariophyllaceae) andMimulus guttatus (Scrophulariaceae). Am J Bot 72: 1700–1706
Searcy KB, Mulcahy DL (1985b) The parallel expression of metal tolerance in pollen and sporophyte ofSilene dioica (L.) Clairv.,Silene alba (Mill.) Krause andMimulus guttatus D.C.. Theor Appl Genet 69:597–602
Searcy KB, Mulcahy DL (1990) Comparison of the response to aluminium toxicity in gametophyte and sporophyte of four tomato (Lycopersicon esculentum Mill.) cultivars. Theor Appl Genet 80: 289–295
Shimabukuro RH (1985) Detoxication of herbicides. In Duke SO (ed) Weed physiology, vol. 2. CRC Press, Boca Raton, Fla., 215–240
Shivanna KR, Sawhney VK (1993) Pollen selection forAlternaria resistance in oilseed brassicas: responses of pollen grains and leaves to a toxin ofA. brassicae. Theor Appl Genet 86:339–344
Smith GA (1986) Sporophytic screening and gametophytic verification of phytoxin tolerance in sugarbeet (Beta vulgaris L.). In Mulcahy DL, Mulcahy GB, Ottaviano E (eds) Biotechnology and ecology of pollen. Springer, Berlin Heidelberg New York, 83–88
Wych RD, Schoper JB (1987) Evaluation of herbicide tolerance of corn inbred lines. In: Wilkinson D (ed) Proc 42nd Ann Corn Sorghum Res Conf. Washington DC, pp 141–160
Yenne SP, Hatzios KK (1990a) Molecular comparison of selected herbicides and their safeners by computer-aided molecular modeling. J Agric Food Chem 38:1950–1956
Yenne SP, Hatzios KK (1990b) Influence of oxime ether safeners on glutathione content and glutathione-related enzyme activity in seeds and seedlings of grain sorghum. Z Naturforsch 45C:96–106
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Communicated by P. L. Pfahler
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Frascaroli, E., Galletti, S. & Landi, P. Haplo-diploid gene expression and pollen selection for tolerance to acetochlor in maize. Theoret. Appl. Genetics 88, 780–784 (1994). https://doi.org/10.1007/BF01253986
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DOI: https://doi.org/10.1007/BF01253986