Summary
A modified diallel cross is used to estimate effects of alleles at the esterase 6 locus, relative to strain and environmental variance, in Drosophila melanogaster. Three strains homozygous for Est 6 s and three homozygous for Est 6 F were crossed in all 36 combinations. Male progeny were scored for mating speed, copula duration and esterase 6 enzyme activity, and all progeny for developmental time. These alleles show a significant additive effect on mating speed, but not on the other traits. Copula duration, developmental time and enzyme activity show additive strain genetic variance. Enzyme activity and developmental time also have maternal or X-chromosome strain variance, and these two traits are significantly correlated. This modified diallel method is generally useful because it permits the partition of trait variance into additive and dominant locus, background genetic and environmental components.
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References
Aslund SE, Rasmuson M (1976) Mating behavior as a fitness component in maintaining allozyme polymorphisms in Drosophila melanogaster. Hereditas 82:175–178
Birley AJ, Marson A, Phillips LC (1980) Genetical variation for enzyme activity in a population of Drosophila melanogaster. 5. The genetical architecture, as shown by diallel analysis, of alcohol dehydrogenase activity. Heredity 49:251–268
Clarke B (1973) The contribution of ecological genetics to evolutionary theory: detecting the direct effects of natural selection on particular polymorphic loci. Genetics 79:101–113
Cochrane BJ, Richmond RC (1979) Studies of esterase 6 in Drosophila melanogaster. 2. The genetics and frequency distribution of naturally occurring variants studied by electrophoretic and heat stability criteria. Genetics 93:461–478
Danford ND, Beardmore JA (1980) Selection at the esterase-6 locus in Drosophila melanogaster by added enzyme substrates in the culture medium. Genetica 50:171–178
Fulker DW (1966) Mating speed in male Drosophila melanogaster: a psychogenetic analysis. Science 153:203–205
Gilbert DG (1981) Studies of esterase 6 in Drosophila melanogaster. 8. Ejaculate esterase 6 and initial sperm use by females. J Insect Physiol 27:641–650
Gilbert DG (1985) Estimating single gene effects on quantitative traits. 2. Statistical properties of five experimental methods. Theor Appl Genet 69:631–636
Gilbert DG, Richmond RC (1982) Esterase 6 in Drosophila melanogaster: reproductive function of active and null males at low temperature (series X). Proc Natl Acad Sci USA 79:2962–2966
Gilbert DG, Richmond RC, Sheehan KB (1981 a) Studies of esterase 6 in Drosophila melanogaster. 5. Progeny production and sperm use in females inseminated by males having active or null alleles. Evolution 35:21–37
Gilbert DG, Richmond RC, Sheehan KB (1981 b) Studies of esterase 6 in Drosophila melanogaster. 7. Remating times of females inseminated by males having active or null alleles. Behav Genet 11:195–208
Griffing B (1956a) Concept of general and specific combining abilities in relation to diallel crossing systems. Aust J Biol Sci 9:463–493
Griffing B (1956b) A generalized treatment of the use of diallel crosses in quantitative inheritance. Heredity 10:31–51
Hayman BI (1954) The analysis of variance of diallel tables. Biometrics 10:235–244
Mane SD, Tompkins L, Richmond RC (1983) A sex pheromone is synthesized in the reproductive tract of Drosophila melanogaster females by an enzyme and substrate provided by males. Science 222:419–421
McDonald JF, Anderson SM, Santos M (1980) Biochemical differences between the products of the Adh locus in Drosophila. Genetics 95:1013–1022
Parsons PA (1964) A diallel cross for mating speed in Drosophila melanogaster. Genetica 35:141–151
Parsons PA, Hosgood SMW, Lee BTO (1967) Poly gene and polymorphism. Mol Gen Genet 99:165–170
Richmond RC, Gilbert DG, Sheehan KB, Gromko MH, Butterworth FM (1980) Esterase 6 and reproduction in Drosophila melanogaster. Science 207:1483–1485
Richmond RC, Tepper CS (1983) Genetic and hormonal regulation of esterase 6 activity in male Drosophila melanogaster. In: Rattazzi MC, Scandalios JG, Whitt GS (eds) Isozymes: current topics in biological and medical research, vol 9. Gene expression and development. Alan R Liss, New York
Robertson FW (1960) The ecological genetics of growth in Drosophila. 1. Body size and developmental time on different diets. Genet Res 1:288–304
Sheehan KB, Richmond RC, Cochrane BJ (1979) Studies of esterase 6 in Drosophila melanogaster. 3. The developmental pattern and tissue distribution. Insect Biochem 9: 443–450
Tepper CS, Richmond RC, Terry AL, Senior A (1982) Studies of esterase 6 in Drosophila melanogaster. 11. Modification of esterase 6 activity by unlinked genes. Genet Res 40:109–125
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Communicated by J. S. F. Barker
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Gilbert, D.G. Estimating single gene effects on quantitative traits. Theoret. Appl. Genetics 69, 625–629 (1985). https://doi.org/10.1007/BF00251114
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DOI: https://doi.org/10.1007/BF00251114