Summary
Tomato callus clones selected for the ability to grow at paraquat concentrations lethal to wild-type cells were found at an approximate frequency of 5×10−8 per viable cell. Diploid plants were regenerated from nine of the nineteen paraquat-tolerant callus clones isolated. Although some of these plants appeared normal, others had altered morphology and reduced vigor and fertility. New callus cultures initiated from these regenerated plants typically had at least a 30-fold increase over the wild type in tolerance to paraquat. Tests on callus from sexual progeny showed that the paraquat-tolerant phenotypes of clones PQT4, PQT6, and probably also PQT13 resulted from dominant nuclear mutations, but the number of loci involved is not yet known. Paraquat spray experiments indicated that slight paraquat-tolerance was expressed at the plant level in PQT13, but not in any of the other clones tested.
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Literature
Barton, D.W., Butler, L.; Jenkins, J.A.; Rick, C.M.; Young, P.A. (1955): Rules for nomenclature in tomato genetics-including a list of known genes. J. Hered. 46, 22–26
Bayliss, M.W. (1980): Chromosomal variation in plant tissues in culture. Int. Rev. Cytol. Suppl. 11 A, 113–144
Clayberg, C.D.; Butler, L.; Kerr, E.A.; Rick, C.M.; Robinson, R.W. (1966): Third list of the known genes in the tomato — with revised linkage map and additional rules. J. Hered. 57, 188–196
D'Amato, F. (1978): Chromosome number variation in cultured cells and regenerated plants. In: Frontiers of plant tissue culture 1978 (ed. Thorpe, T.A.), pp. 287–295. Calgary, Canada: Int. Ass. Plant Tissue Culture 1978
Dix, P.J. (1977): Chilling resistance is not transmitted sexually in plants regenerated from Nicotiana sylvestris cell lines. Z. Pflanzenphysiol. 84, 223–226
Flick, C.E.; Jensen, R.A.; Evans, D.A. (1981): Isolation, protoplast culture and plant regeneration of PFP-resistant variants of Nicotiana tabacum Su/Su. Z. Pflanzenphysiol. 103, 239–245
Gressel, J. (1979): Genetic herbicide resistance: projections on appearance in weeds and breeding for it in crops. In: Plant regulation and world agriculture (ed. Scott, T.K.). NATO Advanced Study Institute Series A, vol. 22, 85–109
Harper, D.B.; Harvey, B.M.R. (1978): Mechanism of paraquat tolerance in perennial ryegrass. 2. Role of superoxide dismutase, catalase and peroxidase. Plant, Cell Environment 1, 211–215
Khush, G.S.; Rick, C.M. (1966): The origin, identification and cytogenetic behavior of tomato monosomics. Chromosoma 18, 407–420
Khush, G.S.: Rick, C.M. (1967): Tomato tertiary trisomics: origin identification, morphlogy and use in determining position of centromeres and arm location of markers. Can. J. Genet. Cytol. 9, 610–631
Khush, G.S.; Rick, C.M. (1968): Tomato telotrisomics: origin, indentation and use in linkage mapping. Cytologia 33, 137–148
Khush, G.S.; Rick, C.M. (1969): Tomato secondary trisomics: origin, identification, morphology, and use in cytogenetic analysis of the genome. Heredity 24, 129–146
Maliga, P. (1980): Isolation, characterization and utilization of mutant cell lines in higher plants. Int. Rev. Cytol. Suppl. 11A, 225–250
Maliga, P. (1981): Streptomycin resistance is inherited as a recessive Mendelian trait in a Nicotiana sylvestris line. Theor. Appl. Genet. 60, 1–3
Maliga, P.: Lázár, G.: Sváb, Z.; Nagy, F. (1976): Transient cycloheximide resistance in a tobacco cell line. Mol. Gen. Genet. 149, 267–271
Malmberg, R.L. (1979): Regeneration of whole plants from callus cultures of diverse genetic lines of Pisum sativum L. Planta 146, 243–244
Meins, F. Jr.; Binns, A.N. (1979): Cell determination in plant development. Bioscience 29, 221–225
Meredith, C.P. (1978): Selection and characterization of aluminum-resistant variants from tomato cell cultures. Plant Sci. Lett. 12, 25–34
Meredith, C.P.; Carlson, P.S. (1982): Herbicide resistance in plant cell cultures. In: Herbicide resistance in plants (eds. LeBaron, H.M. and Gressel, J.). Chichester: J. Wiley
McCoy, T.J.; Bingham, E.T. (1977): Regeneration of diploid alfalfa plants from cells grown in suspension culture. Plant Sci. Lett. 10, 59–66
Miles, C.D. (1976): Selection of diquat resistance photosynthesis mutants from maize. Plant Physiol. 57, 284–285
Miller, O.K.; Hughes, K.W. (1980): Selection of paraquat-resistant variants of tobacco from cell cultures. In Vitro 16, 1085–1091
Miller, P.D.; Vaughn, K.C.; Wilson, K.G. (1980): Induction, ultrastructure, isolation and tissue culture of chlorophyll mutants in carrot. In Vitro 16, 823–828
Milller, A.J.; Grafe, R. (1978): Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol. Gen. Genet. 161, 67–76
Murashige, T. (1974): Plant propagation through tissue culture. Ann. Rev. Plant Physiol. 25, 135–166
Murashige, T.; Skoog, F. (1962): A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497
Padmanabhan, V.; Paddock, E.F.; Sharp, W.R. (1974): Plantlet formation from Lycopersicon esculentum leaf callus. Can. J. Bot. 52, 1429–1432
Ramulu, K.S.; Devreux, M.; Ancora, G.; Laneri, U. (1976): Chimerism in Lycopersicum peruvianum plants regenerated from in vitro cultures of anthers and stem internodes. Z. Pflanzenzücht. 76, 299–319
Reinert, J.; Bajaj, Y.P.S.; Zbell, B. (1977): Aspects of organization — organogenesis, embryogenesis, cytodifferentiation. In: Plant tissue and cell culture (Botanical Monographs vol. 11, 2nd edition) (ed. Street, H.E.), pp. 389–427. Berkeley, Los Angeles: University of California Press
Rick, C.M.; Barton, D.W. (1954): Cytological and genetical identification of the primary trisomics of the tomato. Genetics 39, 640–666
Rosset, R.; Gorini, L. (1969): A ribosomal ambiguity mutation. J. Mol. Biol. 39, 95–112
Shaw, W.C.; Swanson, C.R. (1951): Techniques and equipment used in evaluating chemicals for herbicidal properties. Weeds 1, 352–365
Street, H.E. (1977): Cell (suspension) cultures-techniques. In: plant tissue and cell culture (Botanical Monographs vol. 11, 2nd edition) (ed. Street, H.E.), pp. 92–93. Berkeley, Los Angeles: University of California Press
Summers, L.A. (1980): The bipyridylium herbicides. London, New York: Acad. Press
Thomas, B.R.; Pratt, D. (1981a): Fifficient hybridization between Lycopersicon esculentum and L. peruvianum via embryo callus. Theor. Appl. Genet. 59, 215–219
Thomas B.R.; Pratt, D. (1981b): Breeding tomato strains for use in cell culture research. Plant Mol. Biol. Newslett. 2, 102–105
Thomas, E.; King, P.J.; Potrykus, I. (1979): Improvement of crop plants via single cells in vitro — an assessment. Z. Pflanzenzücht. 82, 1–30
Widholm, J.M. (1972): The use of fluorescein diacetate and phenosafranin for determining viability of cultured plant cells. Stain Techol. 47, 189–194
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Communicated by P. Maliga
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Thomas, B.R., Pratt, D. Isolation of paraquat-tolerant mutants from tomato cell cultures. Theoret. Appl. Genetics 63, 169–176 (1982). https://doi.org/10.1007/BF00303704
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DOI: https://doi.org/10.1007/BF00303704