Summary
All diploid plants (doubled-haploid plants: D.H.) regenerated by androgenesis from binucleated pollen grains in Nicotiana sylvestris differ genetically from the original line as far as morphological features and growth rates are concerned. This androgenic variation (A.V.) is under nuclear control and is transmitted continuously by some D.H. for at least four generations of selfing; other D.H. progenies segregate. Further androgeneses carried out on one single D.H. reveal a new variability and increase the drift from the original line. All results cannot be explained by the presence of residual heterozygosity in the original line, and we suggest that most of the A.V. could originate from changes that occur in the DNA of the vegetative pollen grain cell. D.H. resulting from endomitosis of the vegetative cell would be ‘homozygous’ and stable, whereas D.H. resulting from nuclear fusion between a vegetative and a generative cell would be ‘heterozygous’ and would segregate in seeds through succeeding generations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Arcia, M.A.; Wernsman, E.A.; Burk, L.G. (1978): Performance of anther-derived dihaploids and their conventionally inbred parents as lines, in F1 hybrids and in F2 generations. Crop Sci. 18, 413–418
d'Amato, F. (1977): Cytogenetics of differentiation in tissue ancell cultures. In: Plant cell, tissue and organ culture (ed.: Reinert, J.; Bajaj, Y.P.S., pp. 342–357 Berlin, Heidelberg, New York, Springer
Bourgin, J.P.; Nitsch, J.P. (1967): Obtention de Nicotiana haploides à partir d'étamines cultivées in vitro. Ann. Physiol. Vég. 9 (4), 377–382
Burk, L.G.; Matzinger, D.F. (1976): Variation among anther derived doubled-haploids from an inbred line of tobacco. J. Hered. 67, 381–384
Collins, G.B.; Legg, P.D. (1974): Breeding allopolyploids. In: Haploids in higher plants (ed.: Kasha, K.S.), pp. 231–247. Guelph. Ontario: Univ. Guelph Press
De Paepe, R. (1977): Modifications héréditaires présentées par des haploides doublés obtenus par androgenèse chez N. sylvestris. Thèse de 3è cycle, Univ. Paris XI Orsay
De Paepe, R.; Pernes, J. (1978): Exemples de variations à hérédité mendélienne induites au cours du développement des plantes. Physiol. Vég. 16 (2), 195–204
De Paepe, R.; Nitsch, C.; Godard, M.; Pernes, J. (1976): Potential from haploid and possible use in agriculture. In: Plant tissue culture and its biotechnological application, pp. 341–352. Berlin, Heidelberg, New York: Springer
Devreux, M.; Saccardo, F. (1972): Ibridazine di linee isogenische di tobacco obtenute da cultura in vitro di antere. Genet. Agraria 26, 143–146
Engvild, K.C. (1974): Plantlet ploidy and flower bud size in tobacco anther culture. Hereditas 76, 320–322
Jensen, C.S. (1974): Chromosome doubling techniques in haploids. In: Haploids in higher plants (ed.: Kasha, K.S.) pp. 153–190. Guelph, Ontario: Univ. Guelph Press
Kameya, T.; Hinata, K. (1970): Induction of haploid plants from pollen grains of Brassica. Jpn. J. Breed. 20, 82–87
Knop, W. (1865): Quantitative Untersuchung über den Ernährungs-Process der Pflanzen. Landw. Versuchsstat. 7, 93107
Lauth, M.R.; Spear, B.B.; Heumann, J.; Prescott, D.M. (1976): DNA of ciliated protozea: DNA sequence diminution during macronuclear development in Oxytricha. Cell 7, 67–74
Lin, M.L.; Stabas (1961): Peppermint and spearmint tissue culture. In: Callus formation in submerged culture. Lloydia, 24, 139–145.
McComb, J.A.; McComb, A.J. (1977): The cytology of plantlets derived from cultured anthers of Nicotiana sylvestris. New Phytol. 79, 679–688
Niizeki, H.; Oono, K. (1968): Induction of haploid rice plant from anther culture. Proc. Jpn. Acad. 44, 554–557.
Nitsch, C. (1974): Pollen culture. A New technique for mass production of haploids and homozygous plants. In: proc. int. symp. on haploid in higher-plants (ed.: Kasha, K.S.), pp. 123–135. Guelph, Ontario: Univ. Guelph Press
Nitsch, C. (1977): Culture of isolated microspores. In: plant cell, tissue and organ culture (ed.: Reinert, J.; Bajaj, Y.P.S.), pp. 268–278. Berlin, Heidelberg, New York: Springer
Nitsch, C.; Norreel, B. (1973): Effet d'un choc thermique sur le pouvoir embryogène du pollen de Datura innoxia cultivé dans l'anthère ou isolé de l'anthère. C.R. Acad. Sci. Paris, 276 (D), 303–306
Nitsch, J.P. (1968): Les phytotrons et la bioclimatologie experimentale. Encycl. Pléiade, Vol. physiologie, pp. 1602–1617. Paris: Gallimard
Oinuma, T.; Yoschida, T. (1975): Genetic variation among doubled haploid lines of burley tobacco varieties. Jpn. J. Breed. 24 (5), 211–216
Raschid, A.; Street, H.E. (1974): Segmentation in microspores of Nicotiana sylvestris and Nicotiana tabacum which lead to embryoid formation in anther cultures. Protoplasma 80, 323–334
Ross, J.G. (1965): Somatic chromosome reduction and spectrum mutational effects after colchicine treatment of sorghum. In: The use of induced mutations in plant breeding. pp. 193–203. FAO/IAEA meeting Rome 1964. London: Pergamon Press
Scheffé, H. (1959): The analysis of variance. London: Wiley
Sunderland, N.; Collins, G.B.; Dunwell, J.M. (1974): The role of nuclear fusion in pollen embryogenesis of Datura innoxia Müll. Planta 117, 227–241
Truong, I. (1977): Variabilité des plantes issues d'androgenèse in vitro et tentative d'application directe en sélection de cette variabilité et de la mutagenèse par voie haploide chez le Riz (Oryza sativa). Thèse 3è Cyclce, Univ. Paris XI Orsay
Wenzel, G.; Hoffmann, F.; Thomas, E. (1976): Heterozygous microspore derived plants in rye. Theor. Appl. Genet. 48, 205–208
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
De Paepe, R., Bleton, D. & Gnangbe, F. Basis and extent of genetic variability among doubled haploid plants obtained by pollen culture in Nicotiana sylvestris . Theoret. Appl. Genetics 59, 177–184 (1981). https://doi.org/10.1007/BF00264973
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00264973