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Immunological relationships between Artemia RNA polymerases and between RNA polymerases II from different eukaryotic organisms

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Summary

Rabbit antibodies against Artemia RNA polymerase II have been raised and utilized to study the immunological relationships between the subunits from RNA polymerases I, II and III from this organism and RNA polymerase II from other eukaryotes. We describe here for the first time the subunit structure of Artemia RNA polymerases I and III. These enzymes have 9 and 13 subunits respectively. The anti-RNA polymerase II antibodies recognize two subunits of 19.4 and 18 kDa common to the three enzymes, and another subunit of 25.6 kDa common to RNA polymerases II and III. The antibodies against Artemia RNA polymerase II also react with the subunits of high molecular weight and with subunits of around 25 and 33 kDa of RNA polymerase II from other eukaryotes (Drosophila melanogaster, Chironomus thummi, triticum (wheat) and Rattus (rat)). This interspecies relatedness is a common feature of eukaryotic RNA polymerases.

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Abbreviations

RNAp:

RNA polymerase

DPT:

diazophenylthioether

SDS:

sodium dodecylsulfate

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Authors and Affiliations

  1. Departamento de Bioquímica de la Facultad de Medicina de la U.A.M., Instituto de Investigaciones Biomédicas del C.S.I.C, 28029, Madrid, Spain

    Victor Díaz, Miguel Quintanilla, Jesús Cruces, Jaime Renart & Jesús Sebastián

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  1. Victor Díaz
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  2. Miguel Quintanilla
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  3. Jesús Cruces
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  4. Jaime Renart
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  5. Jesús Sebastián
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Díaz, V., Quintanilla, M., Cruces, J. et al. Immunological relationships between Artemia RNA polymerases and between RNA polymerases II from different eukaryotic organisms. Mol Cell Biochem 76, 123–131 (1987). https://doi.org/10.1007/BF00223477

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  • DOI: https://doi.org/10.1007/BF00223477

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