Skip to main content
SpringerLink
Log in

The interaction between calcium and the activation of Na+, K+-ATPase by noradrenaline

  • Original Articles
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

The interaction of noradrenaline, various cation chelators and calcium on Na+, K+-ATPase from rat cerebral cortex plasma membranes was studied. It was shown that chelation of inhibitory cations by EGTA, EDTA and dipyridyl activated Na+, K+-ATPase to the same extent as noradrenaline but at higher concentrations; increasing concentrations of EGTA depressed the activation by noradrenaline; calcium in the form of a calcium-EGTA buffer depressed Na+, K+-ATPase at physiological concentrations; the inhibition of Na+, K+-ATPase by calcium is dependent on the magnesium concentration in the assay and the inhibition by calcium was partially reversed by noradrenaline.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Skou JC: Biochim Biophys Acta 23:394–401, 1957.

    Google Scholar 

  2. Frankenhäuser B, Hodgkin AL: J Physiol 137:218–244, 1957.

    Google Scholar 

  3. Logan JG, O'Donovan DJ: J Neurochem 27:185–189, 1976.

    Google Scholar 

  4. Schaefer A, Unyi G, Pfeifer AK: Biochem Pharmacol 21:2289–2294, 1972.

    Google Scholar 

  5. O'Fallon JV, Brosemer RW, Harding JW: J Neurochem 36:369–378, 1981.

    Google Scholar 

  6. Logan JG, O'Donovan DJ: Biochem Pharmacol 29:2105–2112, 1980.

    Google Scholar 

  7. Katz AM, Repke DI, Upshaw JE, Polascik MA: Biochim Biophys Acta 205:473–490, 1970.

    Google Scholar 

  8. Portzehl H, Caldwell PC, Rüegg JC: Biochim Biophys Acta 79:581–591, 1964.

    Google Scholar 

  9. Dunham ET, Glynn IM: J Physiol 156:274–293, 1961.

    Google Scholar 

  10. Bader H, Wilkes AB, Jean DH: Biochem Biophys Acta 108:583–593, 1970.

    Google Scholar 

  11. Schaefer A, Komlós M, Seregi A: Biochem Pharmacol 2307–2312, 1979.

  12. Kuo JF, Andersson RGG, Wise BC, Mackerlova L, Salmonsson I, Brackett WL, Katoh N, Shoji M, Wrenn RW: Proc Natl Acad Sci USA 77:7039–7043, 1980.

    Google Scholar 

  13. Godfraind T, Koch M, Verbeke N: Biochem Pharmacol 23:3505–3511, 1974.

    Google Scholar 

  14. Jones DH, Matus AI: Biochim Biophys Acta 356:276–287, 1974.

    Google Scholar 

  15. Petrali EH, Sulakhe PV: Int J Biochem 12:407–420, 1980.

    Google Scholar 

  16. Luft JH: J Biophys Biochem Cytol 9:409–412, 1961.

    Article  CAS  PubMed  Google Scholar 

  17. Berenblum I, Chain E: Biochem J 32:399–415, 1938.

    Google Scholar 

  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: J Biol Chem 193:265–275, 1951.

    CAS  PubMed  Google Scholar 

  19. Deul DH, McIlwain H: J Neurochem 8:246–256, 1961.

    Google Scholar 

  20. Somogyi J: Experientia 20:28–29, 1964.

    Google Scholar 

  21. Epstein FH, Whittam R: Biochem J 99:232–238, 1966.

    Google Scholar 

  22. Walser M: Am J Physiol 200:1099–1104, 1961.

    Google Scholar 

  23. Judah JD, Ahmed K: Nature(London) 194:382, 1962.

    Google Scholar 

  24. Portius HJ, Repke K: Arch exp Path Pharmac 243:335–336, 1962.

    Google Scholar 

  25. Blostein R, Burt VK: Life Sci 10:401–406, 1971.

    Google Scholar 

  26. Davis PW, Vincenzi FF: Life Sci 10:401–406, 1971.

    Google Scholar 

  27. Ogawa Y: J Biochem 64:255–257, 1968.

    Google Scholar 

  28. Hexum TD: Biochem Pharmacol 26:1221–1227, 1977.

    Google Scholar 

  29. Maas JW, Colburn RW: Nature (London) 208:41–46, 1965.

    Google Scholar 

  30. Colburn RW, Maas JW: Nature (London) 208:37–41, 1965.

    Google Scholar 

  31. Rajan KS, Davis JM, Colburn RW: J Neurochem 18:345–364, 1971.

    Google Scholar 

  32. Schaefer A, Seregi A, Komlós M: Biochem Pharmacol 23:2257–2271, 1974.

    Google Scholar 

  33. Schaefer A, Komlós M, Seregi A: Biochem Pharmacol 24:1781–1786, 1975.

    Google Scholar 

  34. Segel GB, Simon W, Lichtman AH, Lichtman MA: J Biol Chem 256:6629–6632, 1981.

    Google Scholar 

  35. Sillén LG, Martell AE: Stability constants of metal-ion complexes (Supplement 1). Allden Press, London, 1971.

    Google Scholar 

  36. Powis DA, Wattus GD: FEBS Letters 126:285–288, 1981.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Physiology, University of Pretoria, 0002, Pretoria

    Marianne Haaga

  2. Dept. of Analytical Chemistry, University of Pretoria, 0002, Pretoria

    Wieland Gevers

  3. Department of Medical Biochemistry, Medical School, U. C. T., Observatory 7925, Cape Town, Republic of South Africa

    Reinhard G. Böhmer

Authors
  1. Marianne Haaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wieland Gevers
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Reinhard G. Böhmer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Haaga, M., Gevers, W. & Böhmer, R.G. The interaction between calcium and the activation of Na+, K+-ATPase by noradrenaline. Mol Cell Biochem 66, 111–116 (1985). https://doi.org/10.1007/BF00220778

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00220778

Keywords

Search

Navigation