Abstract
The levels of DNA adducts in the hepatic tissue of the white sucker fish speciesCatostomus commersoni were determined by32P-postlabelling. The fish were caught at four sites: two sites near the city of Windsor (Québec, Canada) on the St. François River, a downstream tributary of the St. Lawrence River, and two sites in the St. Lawrence River itself, near the city of Montréal (Québec, Canada). The latter sites are known to be contaminated by many pollutants including polycyclic aromatic hydrocarbons. Total adduct levels in all fish ranged from 25.1–178.0 adducts per 109 nucleotides. White sucker from the selected sites of the St. Lawrence River had a significantly higher mean level of DNA adducts than those of the St. François River (129.4 vs 56.8, respectively). These results suggest that the effluents of many heavy industries (e.g. from a Soderberg aluminium plant) flowing in the St. Lawrence River are more likely to produce genotoxic damage to fish than those released in one of its tributary, and mainly associated to the activities of a small town and a nearby pulp and paper mill.
Similar content being viewed by others
References
Grimmer G: Environmental Carcinogens: Polycyclic Aromatic Hydrocarbons. CRC Press, Boca Raton, FL., 1983
Stich HF: Carcinogens and Mutagens in the Environment. CRC press, Boca Raton, FL., vols. 1–3, 1982–1983
International Agency for Research on Cancer: Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. World Health Organization, Lyon, France. 1983
Beland FA, Poirier MC: DNA adducts and carcinogenesis. In Sirica AE (ed.). The pathobiologia of Neaoplasia. Plenum Press, New York, 1989
Singer B, Grunberger D: Molecular Biology of Mutagens and Carcinogens. Plenum Press, New York, 1983
Fowle JR, Sexton K: EPA Priorities for Biologic Markers Research in Environmental Health. Environ. Health Persp., 98: 235–241, 1992
Pelkonen O, Vahakangas D, Nerbert DW: Binding of Polycyclic Aromatic Hydrocarbons to DNA: Comparison with Mutagenesis and Tumorigenesis. J. Toxicol. Environ. Health 6: 1009–1020, 1980
Laliberté D: Teneurs en HAP dans les sédiments près de cinq alumineries du Québec. Direction de la qualité des cours d'eau, Ministère de l'environnement du Québec, 1991
Harris CC, Vahakangas K, Newman MJ, Trivers GE, Shamsuddin A, Sinopoli N, Mann DL, Wright WE: Detection of Benzo[a]pyrene Diol Epoxide-DNA Adducts in Peripheral Blood Lymphocytes and Antibodies to the Adducts in Serum From Coke Oven Workers. Proc. Natl. Acad. Sci. USA, 82: 6672–6676, 1985
Phillips DH, Hemminki K, Alhonen A, Hewer A, Grover PL: Monitoring Occupational Exposure to Carcinogens: Detection by32P-Postlabelling of Aromatic DNA adducts in White Blood Cells from Iron Foundry Workers. Mutat. Res., 204: 531–541, 1988
Santella RM, Hemminki, K, Tang DL, Paik M, Ottman R, Young TL, Savela K, Vodickova L, Dickey C, Whyatt R, Perera FP: Polycyclic Aromatic Hydrocarbon-DNA Adducts in White Blood Cells and Urinary 1-Hydroxypyrene in Foundry Workers. Cancer Epidemiology, Biomarkers and Prevention, 2: 59–62, 1993
Ray S, Dunn BP, Payne JF, Fancey L, Helbig R, Béland P: Aromatic DNA-Carcinogens Adducts in Beluga Whales from the Canadian Artic and Gulf of St. Lawrence. Mar. Poll. Bull. 22: 393–396 1991
Halbrook RS, Kirkpatrick RL, Bevan DR, Dunn BP: DNA Adducts Detected in Muskrats by32P-Postlabeling Analysis. Environ. Toxicol Chem 11: 1605–1613, 1992
Kurelec B, Garg A, Krac S, Chacko M, Gupta RC. Natural Environment Surpasses Polluted Environment in Inducing DNA Damage in Fish. Carciongenesis 10: 1337–1339, 1989
Dunn BP, Black JJ, Maccubbin A:32P-Postlabeling Analysis of Aromatic DNA Adducts in Fish from Polluted Areas. Cancer Res 47: 6543–6548, 1987
Varanasi U, Reichert WL, Stein JE:32P-Postlabeling Analysis of DNA Adducts in Liver of Wild English Sole (Parophrys vetulus) and Winter Flounder (Pseudopleuronectes americanus). J. Cancer Res., 49: 1171–1177, 1989
Liu TY, Cheng SL, Ueng TZ, Chi CW: Comparative Analysis of Aromatic DNA Adducts in Fish from Polluted and Unpolluted Areas by the32P-Postlabeling Analysis. Bull Environ Contamin Toxicol 47: 783–789, 1991
Paul M, Laliberté D: Teneurs en BPC, HAP et pesticides organochlorés dans les sédiments et les poissons des rivières l'Assomption, Richelieu, Yamaska, Saint-François et du Lac Saint-Pierre en 1986. Direction de la qualité des cours d'eau, Ministère de l'environnement du Québec, 1989
Hodson PV, McWhirter M, Ralph K, Gray B, Thivierge D, Carey JH, Van Der Kraak G, Whittle DM, Levesque MC. Effects of Bleached Kraft Mill Effluent on Fish in the St. Maurice River, Quebec. Environ Toxicol Chem 11: 1635–1651, 1992
Laliberté L: Teneurs en Dioxines et furanes dans les poissons en aval de neuf usines de pâtes et papiers et dans leurs effluents en 1988. Direction de la qualité des cours d'eau, Ministère de l'environenment du Québec, 1990
Randerath K, Lu LJW, Li D: A Comparison between Different types of Covalent DNA Modifications (I-Compounds, Persistent Carcinogen Adducts and 5-Methylcytosine) in Regenerating Rat Liver. Carcinogenesis 9: 1843–1848 1988
Randerath K, Reddy MV, Disher RM. Age and Tissue Related DNA Modifications in Untreated Rats: Detection by32P-Postlabeling Assay and Possible Significance for Spontaneous Tumor Incubation and Ageing. Carcinogenesis 7: 1615–1617, 1986
Walsh P, ElAdlouni C, Mukhopadhyay MJ, Nadeau D, Viel G, Poirier GG:32P-Postlabeling determination of DNA adducts in the earthwormLumbricus terrestris exposed to PAH-contaminated soils. Bull Environm Contam Toxicol 54: 654–661, 1995
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
El Adlouni, C., Tremblay, J., Walsh, P. et al. Comparative study of DNA adducts levels in white sucker fish (Catostomus commersoni) from the basin of the St. Lawrence River (Canada). Mol Cell Biochem 148, 133–138 (1995). https://doi.org/10.1007/BF00928150
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00928150