Skip to main content
SpringerLink
Log in

Characterization of an adenovirus early protein required for viral DNA replication: A single strand specific DNA binding protein

  • Review and General Articles
  • a. review articles
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

  1. 1.

    The human adenoviruses types 2, 5 and 12 code for the production of a single strand specific DNA binding protein. The molecular weights of these proteins were 72,000 for types 2 and 5 and 60,000 for type 12. In all three cases proteolytic breakdown fragments of these binding proteins (48,000 MW) were also observed.

  2. 2.

    Analysis of the methionine containing tryptic peptides of these proteins indicate that the types 2 and 5 proteins are similar and clearly distinguishable from the type 12 protein. The peptide maps of these three viral proteins are clearly different from a similar protein found in mock infected cells.

  3. 3.

    Temperature sensitive mutants of type 5 (H5ts125) and type 12 (H12tsA275) adenoviruses fail to produce these proteins at the nonpermissive temperature. H5ts125 infected cells grown at the permissive temperature produce a 72,000 MW protein that is thermolabile, for continued binding to DNA, when compared to type 5 wild type adenovirus 72,000 MW protein. An analysis of the phenotype of this adenovirus mutant indicates that it codes for a viral function at early times after infection that is required for viral DNA replication.

  4. 4.

    Thein vitro translation of adenovirus specific m-RNA results in the synthesis of a small amount of a 72,000 MW protein that binds to single stranded DNA just like the authentic adenovirus DNA binding proteins produced in infected cells.

  5. 5.

    Adenovirus anti-Tumor antigen (T) antiserum from hamsters carrying independently derived adenovirus tumors, have been tested for the presence of antibody to purified DNA binding proteins. One antiserum is positive for these antibodies while the other is negative. These results indicate that some, but not all, adenovirus tumors contain large enough levels of the DNA binding proteins to elicit an antibody response.

  6. 6.

    The type 5 adenovirus temperature sensitive mutant, H5ts125, that codes for a thermolabile DNA binding protein, was complemented or suppressed at the nonpermissive temperature, for the replication of adenovirus DNA, by SV40. SV40tsA temperature sensitive mutants, defective in SV40 DNA replication, do not suppress or complement H5ts125 at the nonpermissive temperature.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Green, M., Ann. Rev. Biochem. 39, 701–756 (1970).

    PubMed  Google Scholar 

  2. Fried, M., Virology 25, 669–671 (1965).

    PubMed  Google Scholar 

  3. Fried, M., Proc. Nat. Acad. Sci. 53, 486–491 (1965).

    PubMed  Google Scholar 

  4. Eckhart, W., Virology 38, 120–125 (1969).

    PubMed  Google Scholar 

  5. Di Mayorca, G., Callender, J., Marin, G. and Giordano, G., Virology 38, 126–133 (1969).

    PubMed  Google Scholar 

  6. Tegtmeyer, P. and Ozer, H., J. Virol. 8, 516–524 (1971).

    PubMed  Google Scholar 

  7. Kimura, G. and Dulbecco, R., Virology 49, 394–403 (1972).

    PubMed  Google Scholar 

  8. Chou, J. and Martin, R., J. Virol. 13, 1101–1109 (1974).

    PubMed  Google Scholar 

  9. Wilkie, N. M., Ustacelebi, S. and Williams, J., Virology 51, 499–503 (1973).

    PubMed  Google Scholar 

  10. Ensinger, M. and Ginsberg, H., J. Virol. 10, 328–339 (1972).

    PubMed  Google Scholar 

  11. Shiroki, K. and Shimojo, H., Virology 61, 474–485 (1974).

    PubMed  Google Scholar 

  12. Williams, J. F., Young, H. and Austin, P. Cold Spring Harbor Symp. Quant. Biol. 39, in press (1974).

  13. Butel, J., Cold Spring Harbor Symp. Quant. Biol. 39, in press (1974).

  14. Levine, A. J., Prog. Med. Virol. 17, 1–37 (1974).

    PubMed  Google Scholar 

  15. Levine, A. J., van der Vliet, P. C. and Sussenbach, J. S., Curr. Topics in Microbiol. and Immunol., in preparation (1975).

  16. Sussenbach, J. S., van der Vliet, P. C., Ellens, D. J. and Jansz, H. S., Nat. New Biol. 239, 47–49 (1972).

    PubMed  Google Scholar 

  17. Sussenbach. J. S., Ellens, D. J. and Jansz, H. S., J. Virol. 12, 1131–1138 (1973).

    PubMed  Google Scholar 

  18. van der Eb, J., Virology 51, 11–23 (1973).

    PubMed  Google Scholar 

  19. Alberts, B. M. and Frey, L., Nature 227, 1313–1318 (1970).

    PubMed  Google Scholar 

  20. Huberman, J., Kornberg, A. and Alberts, B. M., J. Mol. Biol. 62, 39–52 (1971).

    PubMed  Google Scholar 

  21. Sigal, N., Delius, H., Kornberg, T., Gefter, M. and Alberts, B., Proc. Nat. Acad. Sci. 69, 3537–3541 (1972).

    PubMed  Google Scholar 

  22. Herrick, G., Ph.D. Thesis, Princeton University (1973).

  23. Tomizawa, J., Auraku, N. and Iwama, Y., J. Mol. Biol. 21, 247–253 (1966).

    PubMed  Google Scholar 

  24. Broker, T. and Lehman, I., J. Mol. Biol. 60, 131–149 (1971).

    PubMed  Google Scholar 

  25. van der Vliet, P. C. and Levine, A. J., Nat. New Biol. 246, 170–174 (1973).

    PubMed  Google Scholar 

  26. van der Vliet, P. C., Levine, A. J., Ensinger, M. and Ginsberg, H. S., J. Virol. 15, 348–354 (1975).

    PubMed  Google Scholar 

  27. Rosenwirth, B., Shiroki, K., Levine, A. J. and Shimojo, H., Virology, in press (1975).

  28. Levine, A. J., van der Vliet, P. C., Rosenwirth, B., Rabek, J., Frenkel, G. and Ensinger, M., Cold Spring Harbor Symp. Quant. Biol. 39, in press (1974).

  29. Shiroki, K. and Shimojo, H., Virology 45, 163–171 (1971).

    PubMed  Google Scholar 

  30. Shiroki, K., Irisawa, J. and Shimojo, H., Virology 49, 1–11 (1972).

    PubMed  Google Scholar 

  31. Ginsberg, H. S., Williams, J., Doerfler, W. and Shimojo, H., J. Virol. 12, 663–664 (1973).

    PubMed  Google Scholar 

  32. Levine, A. J., Kang, H. S. and Billheimer, F., J. Mol. Biol. 50, 549–568 (1970).

    PubMed  Google Scholar 

  33. Crawford, L. V. and Gesteland, R. F., J. Mol. Biol. 74, 627–634 (1973).

    PubMed  Google Scholar 

  34. Alberts, B. M. and Herrick, G., Methods in Enzymology 21, Academic Press, Inc., New York, 198–217 (1971).

    Google Scholar 

  35. Anderson, C. W., Baum, P. R. and Gesteland, R. F., J. Virol. 12, 241–252 (1973).

    PubMed  Google Scholar 

  36. Anderson, C. W., Lewis, J. B., Atkins, J. F. and Gesteland, R. F., Proc. Nat. Acad. Sci., 71, 2756–2760 (1974).

    PubMed  Google Scholar 

  37. Sever, J. L., J. Immunol. 88, 320–329 (1962).

    PubMed  Google Scholar 

  38. Black, P. H., Lewis, A. M., Jr., Blacklow, N. R., Austin, J. B. and Rowe, W. P., Proc. Nat. Acad. Sci. 57, 1324–1330 (1967).

    PubMed  Google Scholar 

  39. Levinson, A., Rosenwirth, B., Osborn, M., Anderson, S., Levine, A. J. and Weber, K., manuscript in preparation (1975).

  40. Hirt, B., J. Mol. Biol. 26, 365–369 (1967).

    PubMed  Google Scholar 

  41. Levine, A. J. and Ginsberg, H. S., J. Virol. 2, 430–439 (1968).

    PubMed  Google Scholar 

  42. Baum, S. G., Horwitz, M. and Maizel, J., J. Virol. 10, 211–219 (1972).

    PubMed  Google Scholar 

  43. Rosenwirth, B., Anderson, C., Levine, A. J. and Gesteland, R., in preparation (1975).

  44. Rosenwirth, B. and Anderson, C., unpublished observations.

  45. Green, M., Parsons, J. T., Pina, M., Fujinaga, K., Caffier, H. and Landgraf-Leurs, I., Cold Spring Harbor Symp. Quant. Biol. 35, 803–818 (1970).

    Google Scholar 

  46. Pope, J. H. and Rowe, W. P., J. Expt. Med. 116, 1015–1019 (1964).

    Google Scholar 

  47. Tegtmeyer, P., J. Virol. 10, 591–598 (1972).

    PubMed  Google Scholar 

  48. Gardner, S., Field, A., Coleman, D. and Hume, B., Lancet i, 1253–1257 (1971).

    Google Scholar 

  49. Benjamin, T., Curr. Topic Microbiol. and Immunol. 59, 107–133 (1972).

    Google Scholar 

  50. Lewis, A., Levin, M., Wiese, W., Crumpacker, C. and Henry, P., Proc. Nat. Acad. Sci. 63, 1128–1135 (1969).

    PubMed  Google Scholar 

  51. Rowe, W. and Baum, S., Proc. Nat. Acad. Sci. 53, 1340–1347 (1964).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Princeton University Department of Biochemical Sciences, 08540, Princeton, N.J., USA

    A. J. Levine, P. C. van der Vliet, B. Rosenwirth, C. Anderson, J. Rabek, A. Levinson & S. Anderson

  2. Laboratory for Physiological Chemistry, State University of Utrecht, Vondellaan 24A, Utrecht, The Netherlands

    P. C. van der Vliet

  3. Institute of Virology, University of Cologne, Cologne, Germany

    B. Rosenwirth

  4. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA

    C. Anderson

Authors
  1. A. J. Levine
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. C. van der Vliet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Rosenwirth
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Anderson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Rabek
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Levinson
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. S. Anderson
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

An invited article.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Levine, A.J., van der Vliet, P.C., Rosenwirth, B. et al. Characterization of an adenovirus early protein required for viral DNA replication: A single strand specific DNA binding protein. Mol Cell Biochem 11, 79–95 (1976). https://doi.org/10.1007/BF01792789

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01792789

Keywords

Search

Navigation