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In vitro maintenance of the sex pheromone gland of the female Indian meal mothPlodia interpunctella (Hübner)

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Abstract

Sex pheromone glands ofPlodia interpunctella were isolated from surface-sterilized donors of different ages, freed of most of the attached gut, oviduct, and other tissues; rinsed in sterile medium; and cultured in 1 ml of culture medium. The sex pheromone gland cells that were cultured for 10 days in either chemically defined Grace's medium or modified Grace's medium appeared normal in histological examinations. Bioassays of extracted medium in which pheromone glands from mature females had been incubated showed that a greater percentage of the available pheromone was recovered from modified Grace's medium than from chemically defined Grace's medium.

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Author notes

  1. Asoka Srinivasan (NIH-MARC Fellow)

    Present address: Department of Biology, Tougaloo College, 39174, Tougaloo, Mississippi

Authors and Affiliations

  1. Insect Attractants, Behavior and Basic Biology Research Laboratory, Agriculture Research, Science and Education Administration USDA, 32604, Gainesville, Florida

    Asoka Srinivasan (NIH-MARC Fellow), J. A. Coffelt & H. Oberlander

Authors
  1. Asoka Srinivasan
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  2. J. A. Coffelt
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  3. H. Oberlander
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Additional information

Lepidoptera: Pyralidae.

This research was supported in part by NIH Grant 1-F34 GM 06251.

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Srinivasan, A., Coffelt, J.A. & Oberlander, H. In vitro maintenance of the sex pheromone gland of the female Indian meal mothPlodia interpunctella (Hübner). J Chem Ecol 5, 653–662 (1979). https://doi.org/10.1007/BF00986550

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  • DOI: https://doi.org/10.1007/BF00986550

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