Abstract
Antibody to normal mouse catalase will stabilize blood and liver catalase of the acatalasemic mouse against a variety of agents which damage protein tertiary structure (urea, guanidine, trypsin) but not against agents which affect the heme group (azide, hydroxylamine). The antibody will also stabilize catalase against inhibition by 3-amino 1,2,4-triazole (AT), the specific site of action of which is known. The antibody is able also to protect normal mouse catalase from urea denaturation, but it is without effect on AT inhibition of normal catalase. A hypothesis is proposed which explains these results and which helps localize the site of the mutation on the catalase molecule.
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Work supported by the U.S. Atomic Energy Commission.
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Feinstein, R.N., Jaroslow, B.N. & Howard, J.B. Molecular location of the genetic lesion in the acatalasemic mouse. Biochem Genet 6, 263–273 (1972). https://doi.org/10.1007/BF00486120
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DOI: https://doi.org/10.1007/BF00486120