Skip to main content
SpringerLink
Log in

Reaction of common bean callus to culture filtrate of Colletotrichum lindemuthianum: Differences in the composition and toxic activity of fungal culture filtrates

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Culture filtrate of Colletotrichum lindemuthianum caused dark brown lesions on the lower surface veins of bean leaves. This phenomenon was used as a bioassay to study the production of toxic fungal metabolites. Calli from anthracnose-susceptible bean cultivars 'Collacia', 'Andecha' and 'Seronda' were sensitive to a 12.5% solution of race 38 filtrate or to a 25% solution of race 7 filtrate. In contrast, calli from anthracnose-resistant bean genotypes A 247, TU, PI 207262, 'Collacia' × 'Tu', 'Collacia' × AB 136 and 'Collacia' × PI 207262 did not develop browning. Culture filtrates were passed through an ionic-exchange resin and a gel filtration resin. Toxic activity of fractions from two races of the fungal pathogen was different, although in both races slight necrosis was produced by the same nine fractions. Pathogenicity could be related with common substances and toxicity could be identified with differential compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahmed KZ, Mesterhazy A, Bartok T & Sagi F (1996) In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance. 2. Culture filtrate technique and inheritance of Fusarium-resistance in the somaclones. Euphytica 91: 341–349

    Article  Google Scholar 

  • Anderson AJ & Albersheim P (1975) Isolation of a pathogen synthesized fraction rich in glucan that elicits a defense response in the pathogen host. Plant Physiol. 56: 286–291

    Google Scholar 

  • Anderson AJ (1978) Isolation from three species of Colletotrichum of glucan-containing polysaccharides that elicit browning and phytoalexin production in bean. Phytopathology 68: 189–194

    Article  CAS  Google Scholar 

  • Anderson AJ (1980) Differences in the biochemical compositions and toxic activity of extracellular components produced by three races of a fungal plant pathogen, Colletotrichum lindemuthianum. Can. J. Microbiol. 26: 173–179

    Google Scholar 

  • Arai M & Takeuchi M (1993) Influence of Fusarium wilt toxin(s) on Carnation cells. Plant Cell Tiss. Org. Cult. 34: 287–293

    Article  CAS  Google Scholar 

  • Behnke M (1980) General resistance to late blight of Solanum tuberosum plants regenerated from callus resistant to culture filtrates of Phytophthora infestans. Theor. Appl. Genet. 56: 151–152

    Article  Google Scholar 

  • Binarová P, Nedelnik J, Fellner M & Nedbálková B (1990) Selection for resistance to filtrates of Fusarium spp. in embryogenic cell suspension culture of Medicago sativa L. Plant Cell Tiss. Org. Cult. 22: 191–196

    Article  Google Scholar 

  • Carlson PS (1973) Methionine sulfoximine-resistant mutants of tobacco. Science. 180: 1366–1368

    PubMed  CAS  Google Scholar 

  • Collin HA & Dix PJ (1990) Culture systems and selection procedures. In: Dix PJ (ed) Plant Cell Line Selection (pp 3–18). VCH, New York

    Google Scholar 

  • Diaz L & López GF (1986) Evaluación de la resistencia genética de variedades y líneas de frijol Phaseolus vulgaris L. a la antracnosis Colletotrichum lindemuthianum en la Mesa Central. Chapingo 54/55: 28–32

    Google Scholar 

  • Dische Z (1962) Color reaction of carbohydrates. In: Whistler RL & Wolfrom ML (eds) Methods of carbohydrate chemistry, Vol 1 (pp 477–512). Academic Press, New York

    Google Scholar 

  • Dixon RA & Fuller KW (1977) Characterization of components from culture filtrates of Botrytis cinerea which stimulate pahseollin biosynthesis in Phaseolus vulgaris cell suspension cultures. Physiol. Plant Path. 11: 287–296

    CAS  Google Scholar 

  • Fernandez MaT, Fernandez M, Casares C, Rodriguez R & Fueyo M (2000) Bean germplasm evaluation for anthracnose resistance and characterization of agronomic traits: A new physiological strain of Colletotrichum lindemuthianum infecting Phaseolus vulgaris L. in Spain. Euphytica (accepted)

  • Goodman RN (1959) Observations on the production, physiological activity and chemical nature of Colletotin, a toxin from Colletotrichum fuscum. Laub. Pflanzen. Physiol. 37: 187–194

    Google Scholar 

  • Gray IE, Guan YQ & Widholm JM (1986) Reaction of soyben callus to culture filtrates of Phyalophora gregata. Plant Science 47: 45–55

    Article  Google Scholar 

  • Hartman CL, McCoy TJ & Knous T (1984) Selection of alfalfa (Medicago sativa) cell lines and regeneration of plants resistant to the toxin(s) produced by Fusarium oxysporum f.sp. medicaginis. Plant Sci. Lett. 34: 183–194

    Article  Google Scholar 

  • Hidalgo OB, Dematos AP, Cabral RS, Tussel RT, Arzola M, Santos R & Perez MC (1999) Phytotoxic effect of culture filtrate from Fusarium subglutinans the causal agent of fusariose of pineapple (Ananas comosus). Euphytica 104: 73–77

    Article  Google Scholar 

  • Inglis DA, Hagedorn DJ & Rand RE (1988) Use of dry inoculum to evaluate beans for resistance to anthracnose and angular leaf spot. Plant Disease 72: 771–774

    Google Scholar 

  • Jayasankar S & Litz RE (1998) Characterization of embriogenic mango cultures selected for resistance to Colletotrichum gloeosporioides culture filtrate and phytotoxin. Theor. Appl. Gen. 96: 823–831

    Article  CAS  Google Scholar 

  • Jayasankar S, Litz RE, Schnell RJ & Hernandez AC (1998) Embryogenic mango cultures selected for resistance to Colletotrichum gloeosporioides culture filtrate show variation in random amplified polymorphic DNA (RAPD) markers. In Vitro Cell. Devel. Biol. Plant 34: 112–116

    CAS  Google Scholar 

  • Jin H, Hartman GL, Huang YH, Nickell CD & Widholm JM (1996a) Regeneration of soybean plants from embryogenic suspension cultures treated with toxic culture filtrate of Fusarium solani and screening of regenerants for resistance. Phytopathology 86: 714–718

    Google Scholar 

  • Jin H, Hartman GL, Nickell D & Widholm JM (1996b) Phytotoxicity of culture filtrate from Fusarium solani, the causal agent of sudden death syndrome of soybean. Plant Disease 80: 922–927

    Article  Google Scholar 

  • Jones PW (1990) In vitro selection for disease resistance. In: Dix PJ (ed) Plant Cell Line Selection (pp 113–149). VCH, New York

    Google Scholar 

  • Kuti JO, Schading RL, Latigo GV & Bradford JM (1997) Differential responses of guayule (Pharthenium argentatum Gray) genotypes to culture filtrate and toxin from Macrophomina phaseolina. J. Phytopathology 145: 305–311

    CAS  Google Scholar 

  • Lowry OH, Roseborough NH, Farr AL & Randall RJ (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265–275

    PubMed  CAS  Google Scholar 

  • McComb JA, Hinch JM & Clarke AE (1987) Expression on field resistance in callus tissue inoculated with Phytophtora cinnamomi. Phytopathology 77: 346–351

    Google Scholar 

  • Murashige T & Shoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissues cultures. Physiol. Plant. 15: 73–97

    Article  Google Scholar 

  • Nachmias A, Buchner V & Krikun J (1982) Comparison of protein-lipopolyssacharide complexes produced by pathogenic and nonpathogenic strains of Verticillium dahliae Kleb. from potato. Physiol. Plant Path. 20: 213–221

    Article  CAS  Google Scholar 

  • Rapilly F (1968) Les Techniques de Micologie en Patologie Végétale. Annales des Epiphytes, Vol. 19. INRA, Paris

    Google Scholar 

  • Sjödin Ch & Glimelius K (1989) Differences in response to the toxin sirodesmin PL produced by Phoma lingam on protoplasts, cell aggregates and intact plants of resistant and susceptible Brassica accessions. Theor. Appl. Genet. 77: 76–80

    Article  Google Scholar 

  • Tu JC (1986) A detached leaf technique for screening beans (Phaseolus vulgaris L.) in vitro against anthracnose (Colletotrichum lindemuthianum). Can. J. Plant Sci. 66: 805–809

    Article  Google Scholar 

  • Venkatachalam P, Rao KS, Kishor PB & Jayabalan N (1998) Regeneration of late leaf spot-resistant groundnut plants from Cercosporidium personatum culture filtrate-treated callus. Current Science 74: 61–65

    Google Scholar 

  • Vieitez AM, San José C & Vieitez E (1985) In vitro plantlet regeneration from juvenile and mature Quercus robur L. J. Hort. Sci. 60: 99–106

    Google Scholar 

  • Wedge DE & Tainter FH (1997) In vitro detection of Cornus florida callus insensitive to toxic metabolites of Discula destructiva. In Vitro Cell Dev. Biol. Plant 33: 142–146

    CAS  Google Scholar 

  • Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Tech. 47: 189–194

    CAS  Google Scholar 

Download references

Author information

Author notes

  1. M . T. Fernández

    Present address: Laboratorios M, C/ Marqués de Teverga, 7. Esc. Izda. 3° C, 33005, Oviedo, Asturias, Spain

Authors and Affiliations

  1. Laboratorio de Fisiología Vegetal, Universidad de Oviedo, Spain

    M . T. Fernández, M. L. Centeno, M . J. Cañal & R. Rodriguez

  2. Laboratorios M, C/ Marqués de Teverga, 7. Esc. Izda. 3° C, 33005, Oviedo, Asturias, Spain

    M. Fernández

Authors
  1. M . T. Fernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Fernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. L. Centeno
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M . J. Cañal
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Rodriguez
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M . T. Fernández.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fernández, M...T., Fernández, M., Centeno, M.L. et al. Reaction of common bean callus to culture filtrate of Colletotrichum lindemuthianum: Differences in the composition and toxic activity of fungal culture filtrates. Plant Cell, Tissue and Organ Culture 61, 41–49 (2000). https://doi.org/10.1023/A:1006486203315

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006486203315

Search

Navigation