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Simultaneous photoreduction and photooxidation of cytochrome b-559 in Photosystem II treated with carbonylcyanide-m-chlorophenylhydrazone

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Abstract

The possibility of a Photosystem II (PS II) cyclic electron flow via Cyt b-559 catalyzed by carbonylcyanide m-chlorophenylhydrazone (CCCP) was further examined by studying the effects of the PS II electron acceptor 2,6-dichloro-p-benzoquinone (DCBQ) on the light-induced changes of the redox states of Cyt b-559. Addition to barley thylakoids of micromolar concentrations of DCBQ completely inhibited the changes of the absorbance difference corresponding to the photoreduction of Cyt b-559 observed either in the presence of 10 μM ferricyanide or after Cyt b-559 photooxidation in the presence of 2 μM CCCP. In CCCP-treated thylakoids, the concentration of photooxidized Cyt b-559 decreased as the irradiance of actinic light increased from 2 to 80 W m-2 but remained close to the maximal concentration (0.53 photooxidized Cyt b-559 per photoactive Photosystem II) in the presence of 50 μM DCBQ. The stimulation of Cyt b-559 photooxidation in parallel with the inhibition of its photoreduction caused by DCBQ demonstrate that the extent of the light-induced changes of the redox state of Cyt b-559 in the presence of CCCP is determined by the difference between the rates of photooxidation and photoreduction of Cyt b-559 occuring simultaneously in a cyclic electron flow around PS II.

We also observed that the Photosystem I electron acceptor methyl viologen (MV) at a concentration of 1 mM barely affected the rate and extent of the light-induced redox changes of Cyt b-559 in the presence of either FeCN or CCCP. Under similar experimental conditions, MV strongly quenched Chl-a fluorescence, suggesting that Cyt b-559 is reduced directly on the reducing side of Photosystem II.

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Abbreviations

ADRY:

acceleration of the deactivation reactions of the water-splitting system Y

ANT-2p:

2-(3-chloro-4-trifluoromethyl)anilino-3,5-dinitrothiophene

CCCP:

carbonylcyanide-m-chlorophenylhydrazone

DCBQ:

2,6-dichloro-p-benzoquinone

FeCN:

ferricyanide

MV:

methyl viologen

P680 :

Photosystem II reaction center Chl-a dimer

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Author notes

  1. Guy Samson

    Present address: Photosynthesis Research Unit, ARS-USDA, University of Illinois, 1201 W. Gregory Drive, 190 PABL, 61801-3838, Urbana, IL, USA

Authors and Affiliations

  1. Department of Plant Biology, Carnegie Institution of Washington, 290 Panama Street, 49305-1297, Stanford, CA, USA

    Guy Samson & David C. Fork

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  1. Guy Samson
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  2. David C. Fork
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CIW-DPB publication No. 1118.

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Samson, G., Fork, D.C. Simultaneous photoreduction and photooxidation of cytochrome b-559 in Photosystem II treated with carbonylcyanide-m-chlorophenylhydrazone. Photosynth Res 33, 203–212 (1992). https://doi.org/10.1007/BF00030031

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  • DOI: https://doi.org/10.1007/BF00030031

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