Abstract
Studies were carried out with hydroponically grown wheat and cotton to develop the Cu-requiring protein phenolase as a biomarker of Cu nutrient status. Isozymes of phenolase whose levels were reduced by Cu deficiency were identified by Western blots. A competitive enzyme-linked, immunosorbent assay (ELISA) was developed that could detect as little as 25 ng of phenolase. The ELISA revealed that Cu-sufficient cotton leaves had about 4-fold more phenolase antigen than did Cu-sufficient wheat leaves. In both species, the level of phenolase was reduced by 2- to 5-fold in leaves of Cu-deficient plants. Because the immunoassay for phenolase protein is rapid, inexpensive, and can be carried out with small amounts of leaf material, it has potential as a tool for assessment of the Cu status of crop plants.
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Abbreviations
- ELISA:
-
enzyme-linked immunosorbent assay
- HRP:
-
horseradish peroxidase
- TBS:
-
Trisbuffered saline (20 mM Tris-HCl, pH 9.5, 150 mM NaCl)
- TBST:
-
Tris-buffered saline containing 0.05% Tween-20
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Rao, N.R., Ownby, J.D. Development of an ELISA for estimation of the copper nutritional status of wheat and cotton. Plant Soil 155, 453–456 (1993). https://doi.org/10.1007/BF00025081
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DOI: https://doi.org/10.1007/BF00025081