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Molecular characterization of a cytokinin-inducible periwinkle protein showing sequence homology with pathogenesis-related proteins and the Bet v 1 allergen family

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Abstract

Cytokinin treatment of periwinkle callus cultures increased the accumulation of a protein, designated T1, in two-dimensional separated protein extracts. The first 30 NH2-terminal amino acids were determined by Edman degradation and showed significant sequence homology with intracellular pathogenesis-related (IPR) plant proteins and the Bet v 1 allergen family. The deduced amino acid sequence of cDNAs coding for T1, isolated by RT-PCR and 5′ RACE-PCR, exhibited an average sequence identity of 40% with both IPR and Bet v 1-related allergens. T1 and all related proteins contained a p-loop motif typically found in nucleotide-binding proteins as the most conserved sequence feature. Northern blot analysis showed that cytokinin treatment of periwinkle callus induced T1 transcripts, whereas addition of 2,4-dichlorophenoxyacetic acid inhibited this accumulation. Hybridization of genomic periwinkle DNA with the T1 cDNA suggested that the protein is encoded by a single-copy gene. Immunoblot studies with a panel of Bet v 1-specific antibodies and sera from Bet v 1 allergic individuals identified T1 as a protein that is immunologically distinct from the Bet v 1 allergen family and has no allergenic properties.

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Authors and Affiliations

  1. Laboratoire de Biologie Moléculaire et Biochimie Végétale, Faculté de Pharmacie, EA 2106, 37200, Tours, France

    Sabine Carpin, Jean-Claude Chénieux, Marc Rideau & Saïd Hamdi

  2. Institute of General and Experimental Pathology, University of Vienna, 1090, Vienna, Austria

    Sylvia Laffer & Rudolf Valenta

  3. Centre de Biophysique Moléculaire, CNRS, 45071 Orléans cedex 2, France

    Françoise Schoentgen

Authors
  1. Sabine Carpin
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  2. Sylvia Laffer
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  3. Françoise Schoentgen
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  4. Rudolf Valenta
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  5. Jean-Claude Chénieux
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  6. Marc Rideau
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  7. Saïd Hamdi
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Carpin, S., Laffer, S., Schoentgen, F. et al. Molecular characterization of a cytokinin-inducible periwinkle protein showing sequence homology with pathogenesis-related proteins and the Bet v 1 allergen family. Plant Mol Biol 36, 791–798 (1998). https://doi.org/10.1023/A:1005951208815

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