Abstract
The wheat mitochondrial orf25 nucleotide sequence of 576 pb has been determined. Its derived protein sequence shares 88% and 75% amino acid identity with those of maize and tobacco mitochondria, respectively. The wheat and tobacco orf25 sequences lack four inserts, of 6 bp to 36 bp, that are present in the maize homologue. The wheat orf25 gene is actively transcribed and is preceded by a regulatory sequence block very similar to those located upstream of the wheat coxII and atp6 genes. Our observations support the view that orf25 sequences encode a functional polypeptide in plant mitochondria.
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Lonsdale D: The plant mitochondrial genome. In: Marcus A (ed) The Biochemistry of Plants, Vol. 15, pp. 229–295. Academic Press (1989).
Levings CS, Brown GG: Molecular biology of plant mitochondria. Cell 56: 171–179 (1989).
Dewey RE, Levings CS, Timothy DH: Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell 44: 439–449 (1986).
Stamper SE, Dewey RE, Bland MM, Levings CS: Characterization of the gene urf13-T and an unidentified reading frame, ORF25, in maize and tobacco mitochondria. Curr Genet 12: 457–463 (1987).
Bonen L, Boer PH, Gray MW: The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize. EMBO J 3: 2531–2536 (1984).
Bonen L, Bird S: Sequence analysis of the wheat mitochondrial atp6 gene reveals a fused upstream reading frame and markedly divergent N-termini among plant ATP6 proteins. Gene 73: 47–56 (1988).
Wolfe KH, Gouy M, Yang Y-W, Sharp PM, Li W-H: Date of the monocot-dicot divergence estimated from chloroplast DNA sequence data. Proc Natl Acad Sci USA 86: 6201–6205 (1989).
Covello PS, Gray MW: RNA editing in plant mitochondria. Nature 341: 662–666 (1989).
Gualberto JM, Lamattina L, Bonnard G, Weil J-H, Grienenberger J-M: RNA editing in wheat mitochondria results in the conservation of protein sequences. Nature 341: 660–662 (1989).
Heffron F, McCarthy BJ, Ohtsubo H, Ohtsubo E: DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3. Cell 18: 1153–1163 (1979).
Giri I, Danos O, Yaniv M: Genomic structure of the cottontail rabbit (Shope) papillomavirus. Proc Natl Acad Sci USA 82: 1580–1584 (1985).
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Bonen, L., Bird, S. & Belanger, L. Characterization of the wheat mitochondrial orf25 gene. Plant Mol Biol 15, 793–795 (1990). https://doi.org/10.1007/BF00016131
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DOI: https://doi.org/10.1007/BF00016131