Abstract
We describe here a set of binary vectors suitable forAgrobacterium-mediated gene transfer and specially designed for studying plant promoters. These vectors are based on the use of thegus reporter gene, contain multiple unique restriction sites upstream of thegus gene, and minimal promoters for testing the effect of enhancers or activator elements. In addition, an intron-containinggus (uidA) gene was introduced into one of these vectors in order to examine reporter gene activity in tissues whereAgrobacterium contamination may be a problem or in transient expression assays.
References
Bevan M: BinaryAgrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heynecker HL, Boyer HW, Crosa JH, Falkow S: Construction and characterization of new cloning vehicles, II. A multipurpose cloning system. Gene 2: 95–113 (1977).
de Bruijn FJ, Szabados L, Schell J: Chimaeric genes and transgenic plants to study the regulation of genes involved in symbiotic plant-microbe interactions (nodulation genes). Devel Genet 11: 182–196 (1990).
Ebert P, Ha SB, An G: Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays. Proc Natl Acad Sci USA 84: 5745–5749 (1987).
Herrera-Estrella L, Simpson J: Foreign gene expression in plants. In Shaw CH (ed) Plant Molecular Biology: A Practical Approach, pp. 131–160. IRL Press, Eynsham Oxford, England (1988).
Jefferson AR, Burgess SM, Hirsh D:β-Glucuronidase fromEscherichia coli as a gene-fusion marker. Proc Natl Acad Sci USA 83: 8447–8451 (1986).
Kapros T, Stefanov I, Magyar Z, Ocsovszky I, Dudits D: A short histone H3 promoter from alfalfa specifies expression in S-phase cells and meristems. In Vitro Cell Devel Biol 29: 27–23 (1993).
Leung J, Fukuda H, Wing D, Schell J, Masterson R: Functional analysis ofcis-elements, auxin response and early developmental profiles of the mannopine synthase bidirectional promoter. Mol Gen Genet 230: 463–474 (1991).
Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48 (1987).
Metz BA, Welters P, Hoffmann HJ, Jensen EO, Schell J, de Bruijn FJ: Primary structure and promoter analysis of leghemoglobin genes of the stem-nodulated tropical legumeSesbania rostrata: conserved coding sequences,cis-elements andtrans-acting factors. Mol Gen Genet 214: 181–191 (1988).
Odell JT, Nagy F, Chua N-H: Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313: 810–812 (1985).
Pichon M, Journet EP, Dedieu A, de Billy F, Truchet G, Barker DG:Rhizobium meliloti elicits transient expression of the early nodulin gene ENOD12 in the differentiating root epidermis of transgenic alfalfa. Plant Cell 4: 1199–1211 (1992).
Rossi L, Escudero B, Hohn B, Tinland B: Efficient and sensitive assay for T-DNA-dependent transient gene expression. Plant Mol Biol Rep 11: 220–229 (1993).
Schneider M, Kaghad M, Toppan A: EMBL database entry X77672 (1994).
Szabados L, Ratet P, Grunenberg B, de Bruijn FJ: Functional analysis of theSesbania rostrata leghemoglobinglb3 gene 5′-upstream region in transgenicLotus corniculatus andNicotiana tabacum plants. Plant Cell 2: 973–986 (1990).
Szczyglowski K, Szabados L, Fujimoto SY, Silver D, de Bruijn FJ: Site specific mutagenesis of the nodule-infected cell expression (NICE) element and the AT-rich element ATRE-BS2* of theSesbania rostrata leghemoglobinglb3 promoter. Plant Cell 6: 317–332 (1994).
Vancanneyt G, Schmidt R, O'Connor-Sanchez A, Willmitzer L, Rocha-Sosa M: Construction of an introncontaining marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events inAgrobacterium-mediated plant transformation. Mol Gen Genet 220: 245–250 (1990).
Yanisch-Perron C, Vieira J, Messing J: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103–119 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Szabados, L., Charrier, B., Kondorosi, A. et al. New plant promoter and enhancer testing vectors. Mol Breeding 1, 419–423 (1995). https://doi.org/10.1007/BF01248419
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01248419