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Myofibrillar proteins in white muscle of the developing African catfish Heterobranchus longifilis (Siluriforms, Clariidae)

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Abstract

Developmental changes in myofibrillar protein composition were investigated in the myotomal muscle of the African catfish, Heterobranchus longifilis (Clariidae), by several electrophoretic techniques. The main muscle fibres of larvae and the fast-white muscle fibres of juvenile and adult fish were found to express distinct myosin heavy chain and myosin light chain 2 (LC2) isoforms. Three myosin LC2 chains were successively detected, differing by their isoelectric points. In contrast, the alkali light chains remained qualitatively and quantitatively unchanged during fish growth. Actin, α-tropomyosin, and troponin-C (TN-C) were also similar in larval, juvenile, and adult white muscle, but an additional larval tropomyosin isoform was found in the first developmental stages. Two isoforms of troponin-T (TN-T) and troponin-I (TN-I) were synthesised in the course of fish growth. Transition from the larval to the adult isoform was much faster for TN-T than for TN-I. Slow-red muscle myofibrils from adult H. longifilis showed no common component (except actin) with larval, juvenile, or adult fast-white muscle myofibrils. Red myofibrils displayed a single TN-T and a single TN-I isoform, but two isoforms of TN-C. The myofibrillar protein isoforms synthesised at any given developmental stage almost certainly reflect changes in the functional requirements of swimming muscles in the course of fish development.

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Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire et Tissulaire, Université de Liège, rue de Pitteurs 20, B-4020, Liège, Belgique, (Phone

    F. Huriaux & B. Focant

  2. Laboratoire de Morphologie Fonctionnelle, Université de Liège, B-4020, Liège, Belgique

    P. Vandewalle

  3. Laboratoire de Démographie des Poissons et d'Aquaculture, Université de Liège, B-4500, Tihange, Belgique

    E. Baras

  4. IRD (ORSTOM), Groupe aquaculture continentale méditerranéenne et tropicale, F-34033, Montpellier, France

    M. Legendre

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  1. F. Huriaux
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  3. E. Baras
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  4. M. Legendre
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  5. B. Focant
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Huriaux, F., Vandewalle, P., Baras, E. et al. Myofibrillar proteins in white muscle of the developing African catfish Heterobranchus longifilis (Siluriforms, Clariidae). Fish Physiology and Biochemistry 21, 287–301 (1999). https://doi.org/10.1023/A:1007835101472

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