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Protoplast technology for the breeding of top-fruit trees (Prunus, Pyrus, Malus, Rubus) and woody ornamentals

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Summary

Until recently, temperate fruit trees and woody ornamentals have been regarded as recalcitrant to biotechnological breeding approaches based on protoplasts. This however should no longer be the case, as procedures are now available, not only for the regeneration of complete plants from protoplasts of various tissues of such species, but also for the exploitation of protoplast technology for their genetic manipulation. This paper will examine the recent advances and state of the art in this domain, with particular attention to the use of protoplast technology as a novel tool in the breeding of rosaceous top-fruit tree species and woody ornamentals. Problems and their solutions within the context of regenerating plants from isolated protoplasts of stone (Prunus spp.), pome (Pyrus spp., Malus spp.) and small (Rubus spp.) fruits, and of several shrubby ornamental genotypes (Lonicera spp., Weigela spp., Forsythia spp., Cotoneaster spp.) will be addressed. Interspecific (Prunus spinosa + Prunus cerasifera) and intergeneric (Forsythia spp. + Syringa spp.) somatic hybridization within this group of species, as well as the use of protoplasts for host/pathogen interaction studies (Pyrus/Erwinia amylovora) will also be discussed.

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References

  • Bouvier L., Y.X. Zhang & Y. Lespinasse, 1993. Two methods of haploidization in pear, Pyrus communis L.: greenhouse seedling selection and in situ parthenogenesis induced by irradiated pollen. Theor. Appl. Genet. 87: 229–232.

    Article  Google Scholar 

  • Brisset M.N. & J.P. Paulin, 1992. A reliable strategy for the study of disease and hypersensitive reaction induded by Erwinia amylovora. Plant Sci. 85: 171–177.

    Article  CAS  Google Scholar 

  • Brisset M.N., S.J. Ochatt & J.P. Paulin, 1990. Evidence for quantitative responses during co-culture of pear (Pyrus communis L.) protoplasts with Erwinia amylovora (Burrill.) Winslow et al. Plant Cell Rep. 9: 272–275.

    Article  Google Scholar 

  • Brisset, M.N., S.J. Ochatt & J.P. Paulin, 1994. Interactions entre bactéries phytopathogènes et protoplastes végétaux. Abstracts 10éme Collogue sur les Recherches Fruitères, INRA-CTIFL, Angers, France: P14.

  • Duron M. & L. Decoutrye, 1990. In vitro variation in Weigela. p. 606–623. In: Y.P.S. Bajaj (Ed). Biotechnology and Agriculture and Forestry 11: Somaclonal variation in crop improvement I. Springer-Verlag, Berlin.

    Google Scholar 

  • Georges D., L. Decourtye & S.J. Ochatt, 1993. Towards the somatic hybridization of shrubby and climbing honeysuckles. Acta Hort. 326: 337–332.

    Google Scholar 

  • Haberlandt G., 1902. Kulturversuche mit isollierten Pflanzenzellen. Sitzungberichte Akademie der Wissenschaften Wien 111: 69–92.

    Google Scholar 

  • Huancaruna-Perales E. & O. Schieder, 1993. Plant regeneration from leaf protoplasts of apple. Plant Cell Tissue Organ Culture 34: 71–76.

    Article  Google Scholar 

  • Kao K.N. & M.R. Michayluk, 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid medium. Planta 126: 105–110.

    Article  CAS  Google Scholar 

  • Krul W.R., 1988. Recent advances in protoplast culture of horticultural crops: small fruits. Sci. Hort. 37: 267–276.

    Article  Google Scholar 

  • Matthews D., J. Mottley, I. Horan & A.V. Roberts, 1991. A protoplast to plant system in roses. Plant Cell Tissue Organ Culture 24: 173–180.

    Article  Google Scholar 

  • Merkle S.A. & H.E. Sommers, 1987. Regeneration of Liriodendron tulipifera (family Magnoliaceae) from protoplast culture. Amer. J. Bot. 74: 1317–1321.

    Article  Google Scholar 

  • Monier, C. & S.J. Ochatt, 1994. Shoot regeneration from stem-derived callus of Cotoneaster spp. (Rosaceae). Abstracts EUCARPIA Conference. The methodology of plant genetic manipulation: Criteria for decision making. Cork (Ireland), in press.

  • Monier. C., E. Bossis, R. Samson & S.J. Ochatt, 1994. Micropropagation of Cotoneaster spp. is enhanced by the presence of endogenous bacteria. Abstracts IAPTC VIII Int. Congr. Plant Tissue and Cell Culture. Florence, Italy: 66.

  • Murashige T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497.

    Article  CAS  Google Scholar 

  • Ochatt S.I., 1987. Coltura di protoplasti come metodo per il miglioramento genetico nelle piante da frutto. Frutticoltura 49: 58–60.

    Google Scholar 

  • Ochatt S.I., 1990a. Protoplast technology for top-fruit tree breeding. Acta Hort. 280: 215–226.

    Google Scholar 

  • Ochatt S.J., 1990b. Plant regeneration from root callus protoplasts of sour cherry (Prunus cerasus L.). Plant Cell Rep. 9: 268–271.

    Article  Google Scholar 

  • Ochatt S.J., 1991a. Strategies for plant regeneration from mesophyll protoplasts of the recalcitrant fruit and farmwoodland species Prunus avium L. (sweet/wild cherry), Rosaceae. J. Plant Physiol. 139: 155–160.

    CAS  Google Scholar 

  • Ochatt S.J., 1991b. Requirements for plant regeneration from protoplasts of the shrubby ornamental honeysuckle, Lonicera nitida cv. Maïgrun. Plant Cell Tissue Organ Culture 25: 161–167.

    Google Scholar 

  • Ochatt S.J., 1992. The development of protoplast-to-tree systems for Prunus cerasifera and P. spinosa, Rosaceae. Plant Sci. 81: 253–259.

    Article  CAS  Google Scholar 

  • Ochatt S.J., 1993a. Regeneration of plants from protoplasts of Pyrus spp. (pear). p. 105–122. In: Y.P.S. Bajaj (Ed). Biotechnology in Agriculture and Forestry 22: Plant protoplasts and genetic engineering III. Springer-Verlag, Berlin.

    Google Scholar 

  • Ochatt S.J., 1993b. Regeneration of plants from protoplasts of some stone fruits (Prunus spp.). p. 78–96. In: Y.P.S. Bajaj (Ed). Biotechnology in Agriculture and Forestry 23: Plant protoplasts and genetic engineering IV. Springer-Verlag, Berlin.

    Google Scholar 

  • Ochatt S.J., 1993c. An efficient protoplast-to-plant system for the hybrid ornamental shrub, Weigela×florida cv Bristol Ruby. Plant Cell Tissue Organ Culture 33: 315–320.

    Article  CAS  Google Scholar 

  • Ochatt, S.J., 1994. Plant regeneration from protoplasts of Forsythia×intermedia cv. Spring Glory. Abstracts IAPTC VIII Int. Congr. Plant Tissue and Cell Culture. Florence, Italy: 27.

  • Ochatt S.J. & O.H. Caso, 1986. Shoot regeneration from leaf mesophyll protoplasts of wild pear (Pyrus communis var pyraster L.). J. Plant Physiol. 122: 243–246.

    CAS  Google Scholar 

  • Ochatt, S.J. & E.M. Patat-Ochatt, 1991. The time course evolution of viability ad competence for proliferation of woody plant protoplasts following cold-storage. Physiol. Plant. 82: A-16.

  • Ochatt S.J. & E.M. Patat-Ochatt, 1994. Somatic hybridization between Prunus×Pyrus species. p. 455–464. In: Y.P.S. Bajaj (Ed). Biotechnology in Agriculture and Forestry 27: Somatic hybridization in crop improvement III. Springer-Verlag, Berlin.

    Google Scholar 

  • Ochatt S.J. & J.B. Power, 1988a. Rhizogenesis in callus from Conference pear (Pyrus communis L.) protoplasts. Plant Cell Tissue Organ Culture 13: 159–164.

    Article  CAS  Google Scholar 

  • Ochatt S.J. & J.B. Power, 1988b. Plant regeneration from leaf mesophyll protoplasts of Williams' Bon Chretien (Syn. Bartlett) pear (Pyrus communis L.). Plant Cell Rep. 7: 587–589.

    Article  Google Scholar 

  • Ochatt S.J. & J.B. Power, 1988c. An alternative approach to plant regeneration from protoplasts of sour cherry (Prunus cerasus L.). Plant Sci. 56: 75–79.

    Article  Google Scholar 

  • Ochatt S.J. & J.B. Power, 1989a. Selection for salt-drought tolerance using protoplast-and explant-derived tissue cultures of Colt cherry (Prunus avium×pseudocerasus). Tree Physiol. 5: 259–266.

    Google Scholar 

  • Ochatt S.J. & J.B. Power, 1989b. Cell wall synthesis and salt (saline) sensitivity of Colt cherry (Prunus avium×pseudocerasus) protoplasts Plant Cell Rep. 8: 365–367.

    Article  CAS  Google Scholar 

  • Ochatt S.J. & J.B. Power, 1992. Plant regeneration from protoplasts of higher plants. p. 99–127. In: M.W. Fowler, G.S. Warren & M. Moo-Young (Eds). Comprehensive Biotechnology Second Supplement. Pergamon Press, New York.

    Google Scholar 

  • Ochatt S.J., E. Chevreau & M. Gallet, 1992b. Organogenesis from Passe Crassane and Old Home pear (Pyrus communis L.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants. Theor. Appl. Genet. 83: 1013–1018.

    Article  Google Scholar 

  • Ochatt S.J., E.C. Cocking & J.B. Power, 1987. Isolation, culture and plant regeneration of Colt cherry (Prunus avium×pseudocerasus) protoplasts. Plant Sci. 50: 139–143.

    Article  Google Scholar 

  • Ochatt S.J., E.M. Patat-Ochatt & J.B. Power, 1992a. Protoplasts. p. 77–103. In: F.A. Hammerschlag & R.E. Litz (Eds). Biotechnology of Perennial Fruit Crops. CAB International, Oxford.

    Google Scholar 

  • Ochatt S.J., E.L. Rech, M.R. Davey & J.B. Power, 1988b. Long-term effects of electroporation on enhancement of growth and plant regeneration from Colt cherry (Prunus avium×pseudocerasus) protoplasts. Plant Cell Rep. 7: 393–395.

    Article  Google Scholar 

  • Ochatt S.J., P.K. Chand, E.L. Rech, M.R. Davey & J.B. Power, 1988a. Electroporation-mediated improvement of plant regeneration of Colt cherry (Prunus avium×pseudocerasus) protoplasts. Plant Sci. 54: 165–169.

    Article  Google Scholar 

  • Ochatt S.J., E.M. Patat-Ochatt, E.L. Rech, M.R. Davey & J.B. Power, 1989. Somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear (Pyrus communis var pyraster L.) and Colt cherry (Prunus avium×pseudocerasus). Theor. Appl. Genet. 78: 35–41.

    Article  Google Scholar 

  • Oka S. & K. Ohyama, 1985. Plant regeneration from mesophyll protoplasts of paper mulberry (Broussonetia kazinoki Sieb.). J. Plant Physiol. 119: 455–460.

    Google Scholar 

  • Oliveira M.M. & M.S.S. Pais, 1991. Plant regeneration from protoplasts of long-term callus cultures of Actinidia deliciosa var deliciosa cv Hay ward (kiwifruit). Plant Cell Rep. 9: 643–646.

    Article  Google Scholar 

  • Patat-Ochatt E.M., 1994. Regeneration of plants from protoplasts of Malus Xdomestica Borkh. (apple). p. 81–99. In: Y.P.S. Bajaj (Ed). Biotechnology in Agriculture and Forestry 29: Plant protoplasts and genetic engineering V. Springer-Verlag, Berlin.

    Google Scholar 

  • Patat-Ochatt E.M. & J.B. Power, 1990. Advances in plant regeneration from apple protoplasts. Acta Hort. 280: 285–288.

    Google Scholar 

  • Patat-Ochatt E.M., S.J. Ochatt & J.B. Power, 1988. Plant regeneration from protoplasts of apple rootstocks and scion varieties (Malus×domestica Borkh.). J. Plant Physiol. 133: 460–465.

    Google Scholar 

  • Patat-Ochatt E.M., J. Boccon-Gibod, M. Duron & S.J. Ochatt, 1993. Organogenesis of stem and leaf protoplasts of a haploid Golden Delicious apple clone (Malus×domestica Borkh.). Plant Cell Rep. 12: 118–120.

    Article  CAS  Google Scholar 

  • Rech E.L., S.J. Ochatt, P.K. Chand, B.J. Power & M.R. Davey, 1987. Electro-enhancementof division of plant protoplast-derivedcells. Protoplasma 141: 169–176.

    Article  Google Scholar 

  • Rech E.L., S.J. Ochatt, P.K. Chand, B.J. Mulligan, M.R. Davey & J.B. Power, 1988. Electroporation increases DNA synthesis in cultured plant protoplasts. Biotechnology 6: 1091–1093.

    Article  CAS  Google Scholar 

  • Rosati, C. & S.J. Ochatt, 1994. Establishing strategies for stemderived protoplast culture in different Pyrus communis L. genotypes. Abstracts IAPTC VIII Int. Congr. Plant Tissue and Cell Culture, Florence, Italy: 27.

  • Steward F.C., M.O. Mapes & K. Mears, 1958. Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Amer. J. Bot. 45: 653–704.

    Article  Google Scholar 

  • Tsai C.K., 1988. Plant regeneration from leaf callus protoplasts of Actinidia chinensis var chinensis. Plant Sci. 54: 231–235.

    Article  Google Scholar 

  • Vardi A., P. Spiegel-Roy & E. Galun, 1975. Citrus cell culture: isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos. Plant Sci. Lett. 4: 231–236.

    Article  Google Scholar 

  • Wallin A. & L. Johansson, 1989. Plant regeneration from leaf mesophyll protoplasts of in vitro cultured shoots of a columnar apple. J. Plant Physiol. 135: 565–570.

    Google Scholar 

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  1. Station d'Amélioration des Espèces Fruitières et Ornementales, I.N.R.A. Centre d'Angers, B.P. 57, 49071, Beaucouzé Cedex, France

    Sergio J. Ochatt & Estela M. Patat-Ochatt

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  1. Sergio J. Ochatt
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  2. Estela M. Patat-Ochatt
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Ochatt, S.J., Patat-Ochatt, E.M. Protoplast technology for the breeding of top-fruit trees (Prunus, Pyrus, Malus, Rubus) and woody ornamentals. Euphytica 85, 287–294 (1995). https://doi.org/10.1007/BF00023958

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