Summary
Combining ability for six in vitro culture traits in wheat were studied in a 8×8 diallel cross (excluding reciprocals). Specific combining ability effects (sca) were significant for all six traits derived from immature embryos on two media protocols, whereas general combining ability (gca) variances were significant only for five of them. Furthermore, based on ratios obtained by comparing the ratio of K2 gca to K2 sca, sca was more important than gca for all six traits. Genetic correlations between shoot formation and other in vitro traits, except callus weight and root formation, were higher in magnitude than the corresponding phenotypic correlations estimates, indicating the importance of genetic effects.
Similar content being viewed by others
References
BarakatM.N., 1990. Callus induction and plant regeneration form seven cultivars of Brassica napus and their diallel crosses. Alex. J. Agric. Res. 35: 25–37.
BarakatM.N. & E.C.Cocking, 1989. Regeneration of plants from Linum Protoplasts. Biotechnology in Agriculture and Forestry, Vol. 8 (Protoplast and Genetic Engineering I). P. 160–170.
BhaskaranS. & R.H.Smith, 1990. Regeneration in cereal tissue culture: A review. Crop Sci. 30: 1328–1336.
BhullarG.S., K.S.Gill & A.S.Khehra, 1979. Combining ability analysis over F1–F5 generations in diallel crosses of bread wheat. Theor. App. Genet. 55: 77–80.
CarmanJ.G., M.E.Jefferson & W.F.Campbell, 1988. Induction of embryogenic Triticum aestivum L. Calli. 1. Quantification of genotype and culture medium effects. Plant Cell, Tissue and Organ Culture 12: 83–95.
ChevrierN., J.A.Qureshi, P.Hucl & K.K.Kartha, 1990. Heritability of in vitro regeneration in wheat (Triticum aestivum L.). Can. J. Plant Sci. 70: 547–550.
GlebaY.Y. & F.Hoffmann, 1979. ‘Arabidobrassica’ plant genome engineering by protoplast Fusion. Naturwiss. 66: 547–554.
GriffingB., 1956. Concept of general and specific combining ability ir relation to diallel crossing systems. Aust. J. Biol. Sci. 9: 463–493.
LazarM.D., P.S.Baenziger & G.W.Schaeffer, 1984. Cultivar and cultivar x environment effects of the development of callus and polyhaploid plants from anther cultures of wheat. Theor. Appl. Genet. 67: 273–277.
LazarM.D., G.B.Collins & W.E.Vian, 1983. Genetic and environmental effects on the growth and differentiation of wheat somatic cultures. J. Hered. 74: 353–357.
MaddockS.E., V.A.Lancaster, R.Risiott & J.Fronklin, 1983. Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum L.). J. Exp. Bot. 34: 915–926.
MagnussonI. & C.H.Bornman, 1985. Anatomical observations on somatic embryogenesis from scutellar tissues of immature zygotic embryos of Triticum aestivum. Physiol Plant 63: 137–145.
MaligaP., G.Lazar, F.Joo, F.Nagy & L.Menczel, 1977. Restoration of morphogenetic potential in Nicotiana by somatic hybridization. Mol. Gen. Genet. 157: 291–296.
MathiasR.J. & E.S.Simpson, 1986. The interaction of genotype and culture medium on the tissue culture responses of wheat (Triticum aestivum L.) Callus. Plant Cell, Tissue and Organ Culture 7: 31–37.
MollR.H. & C.W.Stuber, 1974. Quantitative genetics empirical results relevant to plant breeders. P. 277–313. In: N.C.Brady (Ed.) Advances in agronomy. Academic Press, New York.
MurashigeT. & F.Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
SearsR.G. & E.L.Deckard, 1982. Tissue culture variability in wheat; callus induction and regeneration. Crop Sci. 22: 546–550.
ShimadaT., 1978. Plant regeneration from the callus induced from wheat embryo. Jpn. J. Genet. 53: 371–374.
Singh, R.K. & B.D. Chaudhary, 1985. Diallel analysis. In: Biometrical methods in quantitative genetic analysis. P. 102–157.
WanY., E.L.Sorensen & G.H.Liang, 1988. Genetic control of in vitro regeneration in alfalfa (Medicago sativa L.) Euphytica 39: 3–9.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Barakat, M.N. Combining abilities of in vitro traits in wheat (Triticum aestivum L.) immature embryo cultures. Euphytica 76, 169–175 (1994). https://doi.org/10.1007/BF00022161
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00022161