Abstract
Seed storage proteins of 131 Japanese Norin wheat (Triticum aestivum) varieties were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis to determine allelic make-up in varieties at each of three loci that control high-molecular-weight (HMW) glutenin subunits. Three alleles were identified at the Glu-A1 locus, six at the Glu-B1 locus and five at the Glu-D1 locus. Twenty-four different, major glutenin HMW subunits were identified and each contained three to five subunits and seventeen different glutenin subunit patterns were observed for 19 subunits in the 131 Japanese Norin varieties. Fourteen alleles were identified by comparison of subunit mobility with that previously found in hexaploid wheat. Japanese Norin varieties showed a specific pattern of allelic variation in glutenin HMW subunits, different from that of Chinese and other country common wheats in allelic frequency at Glu-1 loci.
Similar content being viewed by others
References
Anon, 1983. Descriptive list of varieties of cereals, Maize Oilseeds. Alfred Strophe Verlag, Government Varieties Office, Hannover.
Graybosch, R.A., C.J. Peterson, L.E. Hansen & P.J. Mattern, 1990. Relationships between protein solubility characteristics, 1BL/1RS, high molecular weight glutenin composition, and end-use quality in winter germplasm. Cereal Chem 67: 342–349.
He Zhong-hu, R.J. Pena & S. Rajaram, 1992. High molecular weight glutenin subunitcomposition of Chinese bread wheats. Euphytica 64: 11–20.
Jackson, E.A., L.M. Holt & P.I. Payne, 1983. Characterization of high-molecular-weight gliadin and low molecular weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal location of their controlling genes. Theor Appl Genet. 66: 29–37.
Khan, K., G. Tamminga & O. Lukow, 1989. The effect of wheat flour proteins on mixing and baking-correlations with protein fractions and high molecular weight subunit composition by gel electrophoresis. Cereal Chem 66: 391–396.
Lawrence, G.J., 1986. The high-molecular-weight glutenin subunit composition of Australian wheat cultivars. Aust J Agric Res 37: 125–133.
Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
Lukow, O.M., P.I. Payne & R. Tkachuk, 1989. The HMW glutenin subunit composition of Canadian wheat cultivars and their association with bread-making quality. J Sci Food Agric 46: 451–460.
Miskelly, D.M., 1981. Quality requirements for manufacture of fresh and instant Chinese noodles. Proceedings 31st Annual Conference of the Royal Australian Chemical Institute Cereal Chem Div, Perth, pp. 61–62.
Miskelly, D.M. & H.J. Moss, 1985. Flour quality requirements for Chinese noodle manufacture. J Cereal Sci 3: 379–387.
Morgunov, A.I., W.J. Rogers, E.J. Sayers & E.V. Metakovsky, 1990. The high-molecular-weight glutenin subunit composition of Soviet wheat varieties. Euphytica 51: 41–52.
Nakamura, H., H. Sasaki, H. Hirano & A. Yamashita, 1990a. Ahighmolecular weight subunit of wheat glutenin seed storage protein correlates with its flour quality. Jpn J Breed 40: 485–494.
Nakamura, H., T. Yamada & H. Hirano, 1990b. Geographical variation of glutenin subunit composition of seed storage proteins in wheat cultivars. Jpn J Breed 40 (Suppl. 2): 220–221.
Nakamura, H., H. Yoshida & Y. Amano, 1992. Survey of the Chinese wheat varieties with the good qualities of milling and noodle color. Jpn J Breed 42 (Suppl. 2): 516–517.
Nakamura, H., N. Minaka & A. Inazu, 1996. Differences in the high-molecular-weight glutenin subunits of seed storage protein between Japanese and Chinese Wheats. Jpn J Breed 46 (Suppl. 1): 194.
Ng, P.K.W. & W. Bushuk, 1989. Statistical relationships between high-molecular-weight subunits of glutenin and breadmaking quality of Canadian-grown wheats. Cereal Chem 65: 408–413.
Payne, P.I., L.M. Holt & C.N. Law, 1981. Structural and genetical studies on the highmolecular weigh subunits of wheat glutenin. 1. Allelic variation in subunits amongst varieties of wheat. Theor Appl Genet 60: 229–236.
Payne, P.I. & G.J. Lawrence, 1983a. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11: 29–35.
Payne, P.I., L.M. Holt & G.J. Lawrence, 1983b. Detection of a novel high molecular weight subunit of glutenin in some Japanese hexaploid wheats. J Cereal Sci 1: 3–8.
Payne, P.I., L.M. Holt, E.A. Jackson & C.N. Law, 1984. Wheat storage proteins: theirgenetics and their potential for manipulation by plant breeding. Phil Trans R Soc Lond B304: 359–371.
Stevens, D.J., B.A. Stewart & J.A. Lindley, 1984. Milling and Baking Quality of Home-grown Wheat Varieties: Pocket Guide. Flour Milling and Baking Research Association, Chorleywood, England.
Wang, G., J.W. Snape, H. Hu & W.J. Rogers, 1993. The highmolecular-weight glutenin subunit compositions of Chinese bread wheat varieties and their relationship with bread-making quality. Euphytica 68: 205–212.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nakamura, H., Inazu, A. & Hirano, H. Allelic variation in high-molecular-weight glutenin subunit Loci of Glu-1 in Japanese common wheats. Euphytica 106, 131–138 (1999). https://doi.org/10.1023/A:1003516620466
Issue Date:
DOI: https://doi.org/10.1023/A:1003516620466