Abstract
Data and findings concerning the development of an effective male sterility system (as already applied in the practice of producing tomato hybrid seed) through the use of the positional sterility (ps 2) gene are reported. Undesirable selfing and the necessity of emasculation are discussed as the two main disadvantages that limit the use of ps 2-sterile seed parents in tomato hybrid seed production. The following specific characteristics in the performance of ps 2-lines were evaluated: 1) the percentage of selfing in the ps 2-lines varies within and between the years of growing, being forever lowest during the period of hybrid seed production; 2) the percentage of selfed seeds when using a ps 2-line as seed parent in producing hybrid seed is significantly lower than the percentage of selfing, observed on this same line; 3) the ps 2-gene expressivity varies depending on the genotype. This enables the breeding of ps 2-lines exhibiting very low percentage of selfing; 4) stamen emasculation at anthesis (as applied in ps 2-lines) is significantly easier and more rapid than emasculation in floral buds. The easy maintenance of the sterile lines – by artificial selfing it is possible to produce 100% ps 2 progeny – and the high yield of hybrid seed obtained are discussed as important advantages in using this type of sterility in tomato hybrid seed production.
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References
Atanassova, B., 1991. Linkage studies of the ‘positional sterility-2’ mutant in tomato. J Genetics & Breeding 45: 293–296.
Atanassova, B. & Hr. Georgiev, 1986. Investigation of tomato male sterile lines in relation to hybrid seed production. Acta Horticulture 190: 553–557.
Atanassova, B., M. Antcheva, M. Markova & E. Molle, 1993. Attempt to map loci contributing to low level stigma character in tomato on chromosome 2 using isozyme marker. Proc of the XIIth Eucarpia Meeting on tomato genetics and breeding, Plovdiv, July, 27–31, 1993. pp. 101–105.
Beals, T.P. & R.B. Goldberg, 1997. A novel cell ablation strategy blocks tobacco anther dehiscence. Plant Cell 9(9): 1527–1545.
Clayberg, C.D., 1965. A linked seedling marker for ms 15. Rep Tomato Genet Coop: 15, 29.
Daskaloff, Chr., H. Georgiev, B. Vladimirov, D. Popova, Zh. Danailov, M. Yordanov, T. Strelnikova, T.A. Mashtakova, L. Gousseva & V. Andryushtenko, 1981. Results of Bulgarian-Soviet collaboration in the fields of tomato breeding. Proc Symp ‘Plants Genetics and Cytogenetics’ November, 10–11, XI, 1981, Sofia, pp. 26–34 (in Bulgarian).
Daskalov, S., B. Atanassova, J. Danailov & L. Roussanov, 1997. Gegenwartiger Stand und Ergebnisse der Hybridzuchtung bei Tomato und Paprika in Bulgarien. Proc of the ‘48 Tagung der Vereinigung Osterr. Pflanzenzuchter’ von 25–27 November, 1997, 119–121.
Durand, Y., 1981. Relationship between marker genes aa and Wo and male sterility gene ms 35. In: Compt Rend du Gr travail Eucarpia, Avignon, 18–21, V, 1981, pp. 225–228.
Georgiev, Hr., 1991. Heterosis in tomato breeding. In: Prof Kalloo (Ed), Genetic Improvement of Tomato, pp. 83–98, Springer-Verlag.
Georgiev, Hr. & B. Atanassova, 1981. Positional male sterile line in tomatoes ps 2 with a low level stigma. Compt Rend Acad Bulg Sci 34(3): 423–424.
Georgiev, Hr. & B. Atanassova, 1984. Results from the use of ps 2-sterile lines with short style in tomato hybrid seed production. Proc Symp Eucarpia Tomato, Wageningen, 22–24.05.1984, pp. 32–36.
Georgiev, Hr. & B. Petrakov, 1973. Production of hybrid seeds of Pioneer 2a on the basis of positional male sterility. Gradinarstvo 6: 7–9 (in Bulgarian).
Gorman, S.W. & S. McCormick, 1997. Male sterility in tomato. Crit Rev Plant Sci 16(1): 31–53.
Gueorguiev, Hr. & B. Atanassova, 1981. New opportunities for using male sterility in the tomato. Compt Rend du Gr travail Eucarpia, Avignon, 18–21.V.1981, pp. 221–223.
Kaul, M.L.H., 1987. Male sterility in higher plants. Monograph of Theoret and Appl Genet, Springer-Verlag, 1005 pp.
Lakin, G.F., 1990. Biometrics. Vishaia Shkola, Moscow. 350 pp (in Russian).
Lapushner, D. & R. Frankel, 1967. Practical aspects and the use of male sterility in the production of hybrid tomato seed. Euphytica 16: 300–310.
Oryol, L.I. & M.A. Zhakova, 1977. The mechanism of anther dehiscence of tomato Lycopersicon exculentum Mill (Solanaceae). Bot J LXII 12: 1720–1730 (in Russian).
Pekarkova-Tronickova, E., 1993. History of discovery and exploitation of positional sterility in tomato (Lycopersicon esculentum Mill.) in Czechoslovakia. Zahradnictvi 20(4): 261–165 (in Czech).
Philouze, J., 1974. Genes marqueur lies aux genes de sterilite male ms-32 et ms-35 chez la tomate. Ann Amelior Plantes 24(1): 77–82.
Philouze, J., 1978. Percentage of selfing in a female ps 2 line used for the production of hybrid seed. Rep TGC 28: 13–15.
Philouze, J., 1991. Description of isogenic lines, except for one, or two, monogenically controlled morphological traits in tomato, Lycopersicon esculentum Mill. Euphytica 56: 121–131.
Rick, C.M., 1945. Field identification of genetically male sterile tomato plants to use in producing F1 tomato seeds. Proc Am Soc Hort Sci 46: 277–283.
Sawhney, V.K., 1994. Genetic male sterility in tomato and its manipulation in breeding. In: E.G. Williams et al. (Eds), Genetic control of Self-incompatibility and Reproductive Development in Flowering Plants, pp. 443–458. Kluwer Acad Publ.
Simonov, A.A., 1967. Tomato male sterility and its use in hybrid seed production. Vestnik selsk nauki 2: 27–33 (in Russian).
Stevens, M.A. & C.M. Rick, 1986. Genetics and breeding. In: J.G. Atherton & J. Rudich (Eds), The Tomato Crop, pp. 80–85. Chapman & Hall.
Strelnikova, T., A. Mashtakova, A. Mashtakov, A. Unguryan, Yu. Panchev, Hr. Georgiev & Zh. Danailov, 1988. Estimation of the combining ability of some functional male sterile tomato lines for the development of early-matured F1 hybrids. Proc II National Conf ‘Heterosis in cultivated plants’, 22–23 November, 1988, Sofia, pp. 91–99.
Tanksley, S.D. & D. Zamir, 1988. Double tagging of a male sterile gene in tomato using a morphological marker gene. HortSci 23: 387–388.
Tronickova, E., 1962. New type of functional male sterility in tomato. Ved Prace Vysk Ust Rostl Vyr Praha-Ruzine 6: 29–39 (in Czech).
Tronickova, E. & N. Spirytova, 1981. Production of hybrid seeds from the tomato cultivar Start S-F1 using functional sterility. Rostl Vyr 27: 865–872 (in Czech).
Yordanov, M., 1983. Heterosis in the tomato. In: L. Frankel (Ed), Heterosis — Reappraisal of Theory and Practice, pp. 189–219. Springer-Verlag.
Zhakova, M.A. (Ed), 1977. The mechanism of anther dehiscence in tomato. Actual problems in botany, Kiev, pp. 93–95 (in Russian).
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Atanassova, B. Functional male sterility (ps-2) in tomato (Lycopesicon esculentum Mill.) and its application in breeding and hybrid seed production. Euphytica 107, 13–21 (1999). https://doi.org/10.1023/A:1003527714805
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DOI: https://doi.org/10.1023/A:1003527714805