Abstract
Adult fish of a freshwater population of the Iberian endangered cyprinodontid Aphanius iberus, were induced to reproduce at salinities of 5, 15, 30, 45 and 60 ppt. For each salinity five 30 l aquaria were used, each one including a male and two females. Maturity and spawning outside the natural season, were obtained at conditions of 22 to 28°C and a photoperiod of 14L:10D. The larvae were fed with rotifers Brachionus plicatilis and Synchaeta cecilia valentina. Experiment lasted 40 days. The first spawning occurred on the 17th day at 45 ppt of salinity and the first embryos hatched on the 34th day at 5 and 15 ppt salinity. The final average number of larvae per aquarium ranged from 5.2 (45 ppt salinity) to 10.8 (15 ppt salinity). No significant differences were found between the average values at different salinities (p<0.01).
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Oltra, R., Todolí, R. Reproduction of the Endangered Killifish Aphanius iberus at Different Salinities. Environmental Biology of Fishes 57, 113–115 (2000). https://doi.org/10.1023/A:1007579527064
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DOI: https://doi.org/10.1023/A:1007579527064