Abstract
The question is addressed of how maximal structural NOE data on double labelled proteins can be acquired with a minimal set of NOESY experiments. Two 3D-NOESY spectra are reported which, in concert with other commonly used spectra, provide a convenient strategy for NOE assignment. The 3D CNH-NOESY and 3D NCH-NOESY provide NOE connectivities between amide protons and carbon-bound protons and constitute orthogonal heteronuclear filters which eliminate diagonal signals, considerably improving spectral quality. Two different heteronuclear chemical shift dimensions are recorded in the spectra, thus exploiting the extra dispersion of the heteronucleus and considerably simplifying assignment.
Similar content being viewed by others
References
Bax, A. and Grzesiek, S. (1993) Acc. Chem. Res., 26, 131-138.
Brutscher, B., Morelle, N., Cordier, F. and Marion, D. (1995) J. Magn. Reson., B109, 238-242.
Clore, G.M., Kay, L.E., Bax, A. and Gronenborn, A.M. (1991) Biochemistry, 30, 12-18.
Coles, M., Diercks, T., Liermann, J., Gröger, A., Lupas, A., Peters, J., Koretke, C., Rockel, B., Baumeister, W. and Kessler, H. (1999) Curr. Biol., submitted for publication.
Emsley, L. and Bodenhausen, G. (1992) J. Magn. Reson., 97, 135-148.
Grzesiek, S., Wingfield, P., Stahl, S., Kaufman, J.D. and Bax, A. (1995) J. Am. Chem. Soc., 117, 9594-9595.
Jerala, R. and Rule, G.S. (1995) J. Magn. Reson., B108, 294-298.
Mattiello, D.L., Warren, W.S., Mueller, L. and Farmer II, B.T. (1996) J. Am. Chem. Soc., 118, 3253-3261.
McCoy, M.A. and Mueller, L. (1993) J. Magn. Reson., A101, 122-130.
Morshauser, R.C. and Zuiderweg, E.R.P. (1999) J. Magn. Reson., 139, 232-239.
Muhandiram, D.R., Xu, G.Y. and Kay, L.E. (1993) J. Biomol. NMR, 3, 463-470.
Palmer III, A.G., Cavanagh, J., Wright, P.E. and Rance, M. (1991) J. Magn. Reson., 93, 151-170.
Pascal, S.M., Muhandiram, D.R., Yamazaki, T., Forman-Kay, J.D. and Kay, L.E. (1994) J. Magn. Reson., B103, 197-201.
Vis, H., Boelens, R., Mariani, M., Stroop, R., Vorgias, C.E., Wilson, K.S. and Kaptein, R. (1994) Biochemistry, 33, 14858-14870.
Zhang, O. and Forman-Kay, J.D. (1997) Biochemistry, 36, 3959-3970.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Diercks, T., Coles, M. & Kessler, H. An efficient strategy for assignment of cross-peaks in 3D heteronuclear NOESY experiments. J Biomol NMR 15, 177–180 (1999). https://doi.org/10.1023/A:1008367912535
Issue Date:
DOI: https://doi.org/10.1023/A:1008367912535