Abstract
DNA sequences containing the 5′-flanking regions of the insulin and chymotrypsin genes were linked to the coding sequence of the chloramphenicol acetyltransferase (CAT) gene. The insulin gene recombinant elicits preferential expression of CAT activity when introduced into cells producing insulin; similarly, the chymotrypsin gene recombinant elicits preferential expression in chymotrypsin-producing cells. Sequences located upstream of previously defined transcriptional control elements are essential for efficient expression in both cases.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Alberts, B. et al. in Molecular Biology of the Cell, Ch. 5 (Garland, New York, 1983).
Tsai, S. Y., Tsai, M.-J., Lin, C-T. & O'Malley, B. W. Biochemistry 18, 5726–5731 (1979).
Mather, E. L. & Perry, R. L. Nucleic Acids Res. 9, 6855–6867 (1981).
Derman, E. et al. Cell 23, 731–739 (1981).
Mulligan, R. C., Howard, B. H. & Berg, P. Nature 277, 108–114 (1979).
Pellicer, A. et al. Science 209, 1414–1422 (1980).
Grosschedl, R., Wasylyk, B., Chambon, P. & Birnstiel, M. L. Nature 294, 178–180 (1981).
Wasylyk, B., Kedinger, C., Corden, J., Brison, O. & Chambon, P. Nature 285, 367–373 (1980).
Breathnach, R. & Chambon, P. A. Rev. Biochem. 50, 349–383 (1981).
Grosveld, G. C., Rosenthal, A. & Flavell, R. A. Nucleic Acids Res. 10, 4951–4971 (1982).
Dierks, P. et al. Cell 32, 695–706 (1983).
McKnight, S. L. & Kingsbury, R. Science 217, 316–324 (1982).
Benoist, C. & Chambon, P. Nature 290, 304–310 (1981).
Gruss, P., Dhar, R. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 78, 943–947 (1981).
Banerji, J., Rusconi, S. & Schaffner, W. Cell 27, 299–308 (1981).
Moreau, P., Hen, R., Everett, R., Gaub, P. & Chambon, P. Nucleic Acids Res. 9, 6047–6068 (1981).
Fujimara, F. K., Deininger, P. L., Friedmann, T. & Linney, E. Cell 23, 809–814 (1981).
Katinka, M., Vasseur, M., Montreau, N., Yaniv, M. & Blangy, D. Nature 290, 720–722 (1981).
Spandidos, D. A. & Paul, J. EMBO J. 1, 15–20 (1982).
Chao, M. V., Mellon, P., Charnay, P., Maniatis, T. & Axel, R. Cell 32, 483–493 (1983).
Renkawitz, R. et al. Cell 31, 167–176 (1982).
Oi, V., Morrison, S. L., Herzenberg, L. A. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 80, 825–829 (1983).
Rice, D. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 79, 7862–7865 (1982).
Kondoh, H., Yasuda, K. & Okada, T. S. Nature 301, 440–442 (1983).
Casabadan, M. J. & Cohen, S. N. J. molec. Biol. 138, 179–207 (1980).
Cordell, B. et al. Cell 18, 533–543 (1979).
Lomedico, P. et al. Cell 18, 545–558 (1979).
Bell, G. I. et al. Nature 284, 26–32 (1980).
Ullrich, A., Dull, T., Gray, A., Brosius, J. & Sures, I. Science 209, 612–615 (1980).
Pictet, R. & Rutter, W. J. in Handbook of Physiology, Sect. 7, Vol. 1 (eds Steiner, D. F. & Freinkel, N. ) 25–66 (Williams & Wilkins, Baltimore, 1972).
Gorman, C. M., Moffat, L. F. & Howard, B. H. Molec. cell. Biol. 2, 1044–1051 (1982).
Page, G. S., Smith, S. & Goodman, H. M. Nucleic Acids Res. 9, 2087–2104 (1981).
Santerre, R. F. et al. Proc. natn. Acad. Sci. U.S.A. 78, 4339–4343 (1981).
Jessop, N. W. & Hay, R. J. In Vitro 16, 212 (1980).
Linsley, P. S. & Siminovich, L. Molec. cell. Biol. 2, 593–597 (1982).
Gorman, C., Merlino, G. T., Willingham, M. C., Pastan, I. & Howard, B. Proc. natn. Acad. Sci. U.S.A. 79, 6777–6781 (1982).
Gluzman, Y. Cell 23, 175–182 (1981).
Marion, P. L., Salazar, F. H., Alexander, J. J. & Robinson, W. S. J. Virol. 33, 795–806 (1980).
Wu, C. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 78, 1577–1580 (1981).
Nordheim, A. & Rich, A. Nature 303, 674–679 (1983).
Weiher, H., Konig, M. & Gruss, P. Science 219, 626–631 (1983).
Bell, G. I., Selby, M. J. & Rutter, W. J. Nature 295, 31–35 (1982).
Rotwein, P. et al. Science 213, 1117–1120 (1981).
Owerbach, D. & Nerup, J. Diabetes 31, 275–277 (1982).
Yamamoto, T., de Crombrugghe, B. & Pastan, I. Cell 22, 787–797 (1980).
Robins, D. M., Ripley, S., Henderson, A. S. & Axel, R. Cell 23, 29–39 (1981).
Wickens, M. P., Woo, S., O'Malley, B. W. & Gurdon, J. B. Nature 285, 628–634 (1980).
Dierks, P., van Ooyen, A., Mantei, N. & Weissman, C. Proc. natn. Acad. Sci. U.S.A. 78, 1411–1415 (1981).
Quintrell, N., Hughes, S. H., Varmus, H. E. & Bishop, J. M. J. molec. Biol. 143, 363–393 (1980).
Weintraub, H. & Groudine, M. Science 193, 848–856 (1976).
Burch, J. B. E. & Weintraub, H. Cell 33, 65–76 (1983).
Sakonju, S., Bogenhagen, D. F. & Brown, D. D. Cell 19, 13–25 (1980).
Maniatis, T., Fritisch, E. F. & Sambrook, J. Molecular Cloning—A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).
Graham, F. L. & van der Eb, A. J. Virology 52, 456–467 (1973).
Ullrich, A. et al. Science 196, 1313–1319 (1977).
Alton, N. & Vapnek, D. Nature 282, 864–869 (1979).
Gillies, S. D., Morrison, S. L., Oi, V. T. & Tonegawa, S. Cell 33, 717–728 (1983).
Banerji, J., Olson, L. & Schaffner, W. Cell 33, 729–740 (1983).
Queen, C. & Baltimore, D. Cell 33, 741–748 (1983).
Neuberger, M. S. EMBO J. 2, 1373–1378 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Walker, M., Edlund, T., Boulet, A. et al. Cell-specific expression controlled by the 5′-flanking region of insulin and chymotrypsin genes. Nature 306, 557–561 (1983). https://doi.org/10.1038/306557a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/306557a0
This article is cited by
-
In vivo evaluation of GG2–GG1/A2 element activity in the insulin promoter region using the CRISPR–Cas9 system
Scientific Reports (2021)
-
Mutations in the C1 element of the insulin promoter lead to diabetic phenotypes in homozygous mice
Communications Biology (2020)
-
Efficient, glucose responsive and islet-specific transgene expression by a modified rat insulin promoter
Gene Therapy (2009)
-
Genetic analysis of non-insulin dependent diabetes mellitus in the GK rat
Nature Genetics (1996)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.