Abstract
We have created an insertional mutation that leads to a severe defect in the pattern of limb formation in the developing mouse. The novel recessive mutation is phenotypically identical and non-complementary to two previously encountered limb deformity mutations, and is closely linked to a dominant mutation that gives rise to a related limb dysmorphism. The inserted element thus provides a molecular genetic link with the control of pattern formation in the mammalian embryo.
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References
Schnieke, A., Harbers, K. & Jaenisch, R. Nature 304, 315–320 (1983).
Harbers, K., Kuehn, M., Delius, H. & Jaenisch, R. Proc. natn. Acad. Sci. U.S.A. 81, 1504–1508 (1984).
Wagner, E. R., Covarrubias, L., Stewart, T. A. & Mintz, B. Cell 35, 647–655 (1983).
Palmiter, R. D., Wilkie, T. M., Chen, H. Y. & Brinster, R. L. Cell 36, 869–877 (1984).
Stewart, T. A., Pattengale, P. K. & Leder, P. Cell 38, 627–637 (1984).
Gordon, J. W., Scangos, G. A., Plotkin, D. J., Barbosa, J. A. & Ruddle, F. H. Proc. natn. Acad. Sci. U.S.A. 77, 7380–7384 (1980).
Wagner, E., Stewart, T. & Mintz, B. Proc. natn. Acad. Sci. U.S.A. 78, 5016–5020 (1981).
Wagner, T. E. et al. Proc. natn. Acad. Sci. U.S.A. 78, 6376–6380 (1981).
Harber, K., Jahner, D. & Jaenisch, R. Nature 293, 540–542 (1981).
Costantini, F. & Lacy, E. Nature 294, 92–94 (1981).
Brinster, R. L. et al. Cell 27, 223–231 (1981).
Kalter, H. Teratology 21, 397–429 (1980).
Davisson, M. T. & Roderick, T. H. Cytogenet. Cell Genet. 22, 552–557 (1978).
Cupp, M. B. Mouse News Lett. 26, 51 (1962),
Green, M. C. Mouse News Lett. 26, 34 (1962).
Green, M. C. in Biology of the Laboratory Mouse 2nd edn (ed. Green, E. L.) 87–150 (Dover, New York, 1968).
Kochhar, D. M. in Handbook of Teratology (eds Wilson, J. G. & Fraser, F. C.) 453–479 (Plenum, New York, 1977).
Saunders, J. W. Jr J. exp. Zool. 108, 363 (1948).
Wolpert, L. J. theor. Biol. 25, 1–47 (1969).
Tickle, C., Summerbell, D. & Wolpert, L. Nature 254, 199–202 (1975).
Forsthoefel, P. F. J. Morph. 110, 391–420 (1962).
Goff, S., D'Eustachio, P., Ruddle, F. H. & Baltimore, D. Science 218, 1313–1319 (1982).
Fournier, R. E. K. & Moran, R. G. Somat. Cell Genet. 9, 69–84 (1983).
McLeod, M. J. Teratology 22, 299–301 (1980).
Bates, P. F. & Swift, R. A. Gene 26, 137–146 (1983).
Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold spring Harbor Laboratory, New York, 1982).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
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Woychik, R., Stewart, T., Davis, L. et al. An inherited limb deformity created by insertional mutagenesis in a transgenic mouse. Nature 318, 36–40 (1985). https://doi.org/10.1038/318036a0
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DOI: https://doi.org/10.1038/318036a0
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