Summary
Microwave radiation has been successfully used for hydrolysis of the hepatotoxic cyclic peptides microcystins and nodularin. Set-up of the microwave device and the operating conditions for microwave hydrolysis were optimized. Results of the microwave hydrolysis were compared with results from conventional hydrolysis for 24 h at 110°C.
Microwave hydrolysis of microcystins and nodularin for as little as 10 min at 160°C results in complete cleavage of peptide bonds and high recoveries of amino acids.
Enantioselective determination of amino acids was achieved by use of a previously described HPLC method after pre-column derivatization withortho-phthaldialdehyde and the chiral thiolN-iso-butyryl-cysteine.
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References
K. Sivonen, Phycologia35 (Suppl), 12 (1996).
T. Krishnamurthy, L. Szafraniec, D. F. Hunt, J. Shabanowitz, I. Yates, C. R. Haeur, W. W. Carmichael, O. Skulberg, G. A. Codd, S. Missler, Proc. Natl. Acad. Sci. USA86, 770 (1989).
M. Namikoshi, B. W. Choi, F. Sun, K. L. Rinehart, W. W. Carmichael, W. R. Evans, V. R. Beasley, J. Org. Chem.59, 2349 (1994).
D. P. Botes, P. L. Wessels, H. Kruger, M. T. C. Runnegar, S. Santikarn, R. J. Smith, J. C. J. Barna, D. H. Williams, J. Chem. Soc., Perkin Trans.1, 2747 (1985).
M. Namikoshi, K. L. Rinehart, R. Sakai, R. R. Stotts, A. M. Dahlem, V. R. Beasley, W. W. Carmichael, W. R. Evans, J. Org. Chem.57, 866 (1992).
C. H. W. Hirs, W. H. Stein, S. Moore, J. Biol. Chem.211, 941 (1954).
S. T. Chen, S. H. Chiou, Y. H. Chu, K. T. Wang, Int. J. Peptide Protein Res.30, 572 (1987).
A. Peter, G. Lans, D. Tourwe, E. Gerlo, G. Van Binst, Peptide Res.6, 48 (1993).
C. Hummert, M. Reichelt, C. Legrand, E. Graneli, B. Luckas, Chromatographia (1999), submitted for publication.
S. T. Chiou, K. T. Wang, J. Chromatogr.491, 424 (1989).
C. Woodward, L. B. Gilman, W. G. Engelhart, Int. Lab.20, 40 (1990).
L. Joergensen, H. N. Thestrup, J. Chromatogr. A706, 421 (1995).
E. Marconi, G. Panfili, L. Bruschi, V. Vivanti, L. Pizzoferrato, Amino Acids8, 201 (1995).
H. Engelhardt, M. Krämer, H. Waldhoff, Chromatographia30, 523 (1990).
H. Brückner, R. Wittner, H. Godel, Chromatographia32, 383 (1991).
H. Brückner, T. Westhauser, H. Gode, J. Chromatogr. A711, 201 (1995).
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Reichelt, M., Hummert, C. & Luckas, B. Hydrolysis of microcystins and nodularin by microwave radiation. Chromatographia 49, 671–677 (1999). https://doi.org/10.1007/BF02466910
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DOI: https://doi.org/10.1007/BF02466910