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Stereochemical resolution of racemates, in HPLC, using a chiral stationary phase based upon immobilized α-chymotrypsin. I. Structural chiral separations

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Summary

α-Chymotrypsin was immobilized on an epoxide derivatized silica gel by anin situ immobilization process. Several racemates were resolved by a structural recognition mechanism. The immobilization process and the stability of this α-chymotrypsin stationary phase have been studied. The mobile phase parameters including the ionic strength, pH and the effects of organic modifiers have been also investigated. The retention, efficiency and stereoselectivity of the solutes appear to be related to their molecular structure, hydrophobicity and electrostatic interactions. These relationships determine the recognition mechanism and the position of each enantiomer in the active site.

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  1. ENSCPB, Avenue Pey-Berland, (BP 108), 33 402, Talence, France

    G. Félix & V. Descorps

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  1. G. Félix
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  2. V. Descorps
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Félix, G., Descorps, V. Stereochemical resolution of racemates, in HPLC, using a chiral stationary phase based upon immobilized α-chymotrypsin. I. Structural chiral separations. Chromatographia 49, 595–605 (1999). https://doi.org/10.1007/BF02466900

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  • DOI: https://doi.org/10.1007/BF02466900

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