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Study of the chromatographic behavior and inclusion constants of new fluorescence agents by use of cyclodextrin additives under conditions of steady- and dynamic-state equilibrium and with fluorescence detection

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Summary

We report four new derivatization agents, acridone-N-acetic acid (ARC), carbazole-9-ylacetic acid (CRA), carbazole-9-ylpropionic acid (CRP), and 2-methyl-2-carbazole-9-ylacetic acid (MCRA), with strong fluorescence emission which has low dependence on solvent polarity. The emission maxima for ARC, CRA, CRP, and MCRA were 430 nm (λex 404 nm), 368 nm (λex 335 nm), 356 nm (λex 340 nm) and 360 nm (λex 330 nm), respectively. The effects of mobile-phase composition, pH, and temperature on the liquid chromatographic retention behavior of the four fluorescence agents were investigated. An experimental model was established for calculating the inclusion constants of cyclodextrin (CD) complexes in the dynamic state, using β-cyclodextrin (β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) as examples, and different mobilephase compositions. On the basis of the model, the inclusion constants of the solutes in pure water (K fw) were determined by extrapolation. The thermodynamic parameters (ΔH o and ΔS o) and dissociation constantsK am for the solutes in this chromatographic system were obtained by means of capacity factor (k) values using a corresponding model formulation.

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Authors and Affiliations

  1. Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, 730000, Lanhou, P.R. China

    J. M. You, W. J. Lao, X. J. Fan & Q. Y. Ou

  2. Department of Chemistry, Qufu Normal University, 273165, Qufu Shandong, P. R. China

    X. L. Jia

Authors
  1. J. M. You
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  2. W. J. Lao
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  3. X. J. Fan
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  4. Q. Y. Ou
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  5. X. L. Jia
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You, J.M., Lao, W.J., Fan, X.J. et al. Study of the chromatographic behavior and inclusion constants of new fluorescence agents by use of cyclodextrin additives under conditions of steady- and dynamic-state equilibrium and with fluorescence detection. Chromatographia 49, 95–104 (1999). https://doi.org/10.1007/BF02467195

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  • DOI: https://doi.org/10.1007/BF02467195

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