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Comparative study on determination of fumagillin in fish by normal and reversed phase chromatography

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Summary

Fumagillin is an antibiotic agent mostly used in the veterinary profession. A normal-phase liquid chromatographic method was developed for the determination of this drug in fish matrices. A hexane-dichloromethanedioxan-2-PrOH-acetic acid (43:43:9:5:0.1 v/v %) eluent was used on a Perkin Elmer silica gel column. For validation of the process a reversed-phase HPLC method was also developed (eluent: acetonitrile-water-bicyclohyxylamine 70:30:0.05 v/v %, with Spherisorb ODS, as stationary phase). Recovery is about 100% and the limit of detection is 5 ng g−1 for meat samples.

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Authors and Affiliations

  1. Department of General and Analytical Chemistry, Technical University of Budapest, 1521, Budapest, Hungary

    J. Fekete & Zs. Romvári

  2. Institute of Oecological Chemistry, GSF, Ingolstätter Landstraße 1, 85764, Oberschleißheim, Germany

    I. Gebefügi & A. Kettrup

Authors
  1. J. Fekete
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  2. Zs. Romvári
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  3. I. Gebefügi
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  4. A. Kettrup
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Fekete, J., Romvári, Z., Gebefügi, I. et al. Comparative study on determination of fumagillin in fish by normal and reversed phase chromatography. Chromatographia 48, 48–52 (1998). https://doi.org/10.1007/BF02467515

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  • DOI: https://doi.org/10.1007/BF02467515

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