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Improved separation of diastereoisomers in capillary electrophoresis using a mixture of β-cyclodextrin and sodium taurodeoxycholate

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Summary

2,3,4,6-tetra-O-acetyl-β-D-glucopranosyl isothiocyanate derivatives of a number of basic compounds, containing one chiral center, have been prepared. The indirect resolution of enantiomers was achieved with a high separation efficiency by capillary electrophoresis, using a sodium taurodeoxycholate/β-cyclodextrin system. This methodology was found to be superior to the resolution of the same derivatives by reversed phase HPLC analysis. A possible mechanism for stereoselectivity is described.

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Authors and Affiliations

  1. SmithKline Beecham, The Frythe, AL6 9AR, Welwyn, Herts, UK

    G. N. Okafo, K. K. Rana & P. Camilleri

Authors
  1. G. N. Okafo
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  2. K. K. Rana
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  3. P. Camilleri
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Okafo, G.N., Rana, K.K. & Camilleri, P. Improved separation of diastereoisomers in capillary electrophoresis using a mixture of β-cyclodextrin and sodium taurodeoxycholate. Chromatographia 39, 627–630 (1994). https://doi.org/10.1007/BF02268292

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  • DOI: https://doi.org/10.1007/BF02268292

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