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Retention behavior of some monobasic penicillins and their quaternary ammonium ion pairs in reversed-phase high-performance liquid chromatography

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Summary

The retention behavior of some monobasic penicillins (penicillin G, penicillin V, oxacillin, cloxacillin and dicloxacillin) and their quaternary alkylammonium ion pairs has been studied in a reversed-phase high-performance liquid chromatographic system using Partisil 5 μm ODS-3 asstationary phase and mixtures of acetonitrile and aqueous phosphate buffers as mobile phases. The influence of pH, pairing-ion chain length and concentration, and column temperature on retention has been investigated and the observed behavior of the compounds during the chromatographic process has been discussed.

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Authors and Affiliations

  1. Laboratory of Milk and Milk Products, School of Veterinary Medicine, Aristotle University, 54006, Thessaloniki, Greece

    D. J. Fletouris & A. J. Mantis

  2. Laboratory of Food Hygiene, School of Veterinary Medicine, Aristotle University, 54006, Thessaloniki, Greece

    J. E. Psomas

Authors
  1. D. J. Fletouris
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  2. J. E. Psomas
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  3. A. J. Mantis
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Fletouris, D.J., Psomas, J.E. & Mantis, A.J. Retention behavior of some monobasic penicillins and their quaternary ammonium ion pairs in reversed-phase high-performance liquid chromatography. Chromatographia 32, 436–440 (1991). https://doi.org/10.1007/BF02327974

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  • DOI: https://doi.org/10.1007/BF02327974

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