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Determination of chenodeoxycholic acid on 3-cyanopropyltrichlorosilane-treated high-performance thin-layer chromatographic plates

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Summary

Chenodeoxycholic acid in commerical drugs was analyzed by high-performance, thin-layer chromatography (HPTLC) using a cyanoalkyl chemically-bonded stationary phase, prepared by treating pre-coated silica with 3-cyanopropyltrichlorosilane (3CPTS). The 3CPTS-treated plates were used to evaluate commerical chenodeoxycholic acid in drug capsules. After development with methanol on the 3CPTS-treated plate, the chenodeoxycholic acid spot in the chromatogram was measured with a TLC densitometer equipped with a dual-wave length TLC scanner at λ=370nm. By this method, the fluorescence intensity of chenodeoxycholic acid was measured within 2.6% error over the range 30–240ng and the limit of detection was 30ng.

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Authors and Affiliations

  1. Gifu Prefectural Tajimi Hospital, 5-161, Maehata-cho, 507, Tajimi, Gifu, Japan

    M. Okamoto & H. Kakamu

  2. OM Laboratory, Taisei Kako Co., Ltd., 1165, Tabata, Samukawa-machi, Koza-gun, 253-01, Kanagawa, Japan

    T. Omori

Authors
  1. M. Okamoto
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  2. H. Kakamu
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  3. T. Omori
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Okamoto, M., Kakamu, H. & Omori, T. Determination of chenodeoxycholic acid on 3-cyanopropyltrichlorosilane-treated high-performance thin-layer chromatographic plates. Chromatographia 23, 601–603 (1987). https://doi.org/10.1007/BF02324873

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  • DOI: https://doi.org/10.1007/BF02324873

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