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Prüfung auf Kombinationseffekte von Quercetin mit den Herbiziden Atrazin, Cyanazin und Gesamprim in Mutagenitätstests

Combination effects of quercetin with the herbicides atrazine, cyanazine or gesamprim in mutagenicity tests

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Zusammenfassung

Das in Plfanzen vorkommende Flavonol Quercetin und die zu den Triazinen gehörenden Herbizide Atrazin, Cyanazin und Gesamprim® wurden einzeln und in Kombination miteinander auf genotoxische Wirkungen untersucht. Es wurden die Induktion von Schwesterchromatidaustauschen (SCE-Test) und von Mutationen zur 6-Thioguaninresistenz (HPRT-Test) an Ovarzellen des Chinesischen Hamsters (CHO-Zellen) bestimmt. Während sich in SCE-Test keine Hinweise auf genotoxische Wirkungen ergaben, verursachten die Prüfsubstanzen im HPRT-Test nach metabolischer Aktivierung durch zugesetzte subzelluläre Enzympräparate der Rattenleber (S9-Mix) leicht erhöhte Mutationsraten. Die Kombinationen von zwei oder drei Prüfsubstanzen verursachten keine deutliche Zunahme der genotoxischen Wirkung.

Summary

The plant flavonol quercetin and the triazine herbicides atrazine, cyanazine, and gesamprim were examined individually and in combination for the induction of genotoxic effects. The sister chromatid exchange (SCE) assay and the gene mutation assay for 6-thioguanine resistance (HPRT) were carried out with Chinese hamster ovary (CHO) cells. Whereas no evidence of an increased SCE rate was found, the test substances caused a slightly increased mutation rate in the HPRT assay after metabolic activation with a subcellular liver enzyme preparation. Combination studies with two or three of the test substances did not result in higher mutation rates than those observed for the individual compounds tested singly.

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Authors and Affiliations

  1. Institut für Hygiene und Toxikologie, Germany

    C. Guigas & B. L. Pool-Zobel

  2. Institut für Ernährungsphysiologie der Bundesforschungsanstalt für Ernährung, Karlsruhe

    J. F. Diehl

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  1. C. Guigas
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  2. B. L. Pool-Zobel
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  3. J. F. Diehl
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Guigas, C., Pool-Zobel, B.L. & Diehl, J.F. Prüfung auf Kombinationseffekte von Quercetin mit den Herbiziden Atrazin, Cyanazin und Gesamprim in Mutagenitätstests. Z Ernährungswiss 32, 131–138 (1993). https://doi.org/10.1007/BF01614756

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  • DOI: https://doi.org/10.1007/BF01614756

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