Summary
Pollen tube growth is shown to be inhibited by both suboptimal (<10−4 M) and supraoptimal (>10−3 M) Ca2+ ion concentrations. Tip extension rates are independent of Ca2+ ion concentration over the optimal range. Changes in the structure of the tips of pollen tubes after transfer to inhibitory Ca2+ ion conditions provide evidence in support of our proposed mechanism of pollen tube tip growth (J. theor. Biol.98, 15–20, 1982). It is clearly demonstrated that tip extension is controlled, not only by the rate of vesicle fusion, but also by the state of plasticity of the tip; these processes appear to be sensitive to changes in Ca2+ ion concentration.
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Allen, R. D., Allen, N. S., 1978: Cytoplasmic streaming in amoeboid movement. Ann. Rev. Biophys. Bioeng.7, 469–495.
Baydoun, E. A.-H., Northcote, D. H., 1980: Measurement and characteristics of fusion of isolated membrane fractions from maize root tips. J. Cell Sci.45, 169–186.
Brewbaker, J. L., Kwack, B. H., 1963: The essential role of calcium ion in pollen germination and pollen tube growth. Amer. J. Bot.50, 859–865.
Dahl, G., Gratzl, M., 1976: Calcium-induced fusion of isolated secretory vesicles from the islet of Langerhans. Cytobiologie12, 344–355.
Foreman, J. C., Mongar, J. L., Gomperts, B. D., 1973: Calcium ionophores and movement of calcium ions following the physiological stimulus to a secretory process. Nature245, 249–251.
Koter, M., de Kruijff, B., van Deenen, L. L. M., 1978: Calcium-induced aggregation and fusion of mixed phosphatidylcholine-phosphatidic acid vesicles as studied by31PNMR. Biochim. Biophys. Acta514, 255–263.
Larson, D. A., 1965: Fine structural changes in the cytoplasm of germinating pollen. Amer. J. Bot.52, 139–154.
Mascarenhas, J. P., Machlis, L., 1962: Chemotropic response ofAntirrhinum majus pollen to calcium. Plant Physiol.39, 70–77.
Matthews, E. K., 1979: Calcium translocation and control mechanisms for endocrine secretion. In: Secretory Mechanisms (Hopkins, C. R., Duncan, C. J., eds.), Symposia of the Society for Experimental Biology No. 33, pp. 225–249. Cambridge: Cambridge University Press.
Morré, D. J., van der Woude, W. J., 1974: Origin and growth of cell surface components. In: Macromolecules Regulating Growth and Development (Hay, E. D., King, T. J., Papaconstantinou, J., eds.), 13th Symposium of Society for Developmental Biology, pp. 81–111. New York: Academic Press.
Morris, M. R., Northcote, D. H., 1977: Influence of cations at the plasma membrane in controlling polysaccharide secretion from sycamore suspension cells. Biochem. J.166, 603–618.
Picton, J. M., Steer, M. W., 1981: Determination of secretory vesicle production rates by dictyosomes in pollen tubes ofTradescantia using cytochalasin D. J. Cell Sci.49, 261–272.
— —, 1982: A model for the mechanism of tip extension in pollen tubes. J. theor. Biol.98, 15–20.
Portzehl, H., Caldwell, P. C., Rüegg, J. C., 1964: The dependence of contraction and relaxation of muscle fibres from the crabMaia squinado on the internal concentration of free calcium ions. Biochim. Biophys. Acta79, 581–591.
Pressman, B. C., 1976: Biological applications of ionophores. Annu. Rev. Biochem.45, 501–529.
Reiss, H.-D., Herth, W., 1979: Calcium ionophore A23187 affects localized wall secretion in the tip region of pollen tubes ofLilium longiflorum. Planta145, 225–232.
— —, 1980: Effects of the broad-range ionophore X-537A on pollen tubes ofLilium longiflorum. Planta147, 295–316.
Rosen, W. G., Gawlik, S. R., Dashek, W. V., Siegesmund, K. A., 1964: Fine structure and cytochemistry ofLilium pollen tubes. Amer. J. Bot.51, 61–71.
Taylor, D. L., Condeelis, J. S., Moore, P. L., Allen, R. D., 1973: The contractile basis of amoeboid movement I. The Chemical control of motility in isolated cytoplasm. J. Cell Biol.59, 378–394.
Ueda, T., von Olenhusen, K. G., Wohlfarth-Bottermann, K. E., 1978: Reaction of the contractile apparatus inPhysarum to injected Ca++, ATP, ADP, and 5′AMP. Cytobiologie18, 76–94.
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Picton, J.M., Steer, M.W. Evidence for the role of Ca2+ ions in tip extension in pollen tubes. Protoplasma 115, 11–17 (1983). https://doi.org/10.1007/BF01293575
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DOI: https://doi.org/10.1007/BF01293575