Summary
The protein sequences of seven 3′-aminoglycoside phosphotransferases falling into the six identified types and three 6′-aminoglycoside phosphotransferases were analyzed to give a rooted phylogenetic tree. This tree supports the origin of these groups of enzymes in an ancestor closely related to the actinomycetes, and that horizontal transfer of the resistance genes occurred, possibly via transposons. The implications for genetic engineering of a novel antibiotic are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Benveniste R, Davies J (1973) Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria. Proc Natl Acad Sci USA 70:2276–2280
Brenner S (1987) Phosphotransferase sequence homology. Nature 329:21
Distler J, Braun C, Eberty A, Piepersberg W (1987) Gene cluster for streptomycin biosynthesis inStreptomyces griseus: analysis of a central region including the major resistance gene. Mol Gen Genet 208:204–210
Gil JA, Hopwood DA (1983) Cloning and expression of a p-aminobenzoic acid synthetase gene of the candicidin producingStreptomyces griseus. Gene 25:119–132
Hopwood DA, Chater KF (1984)Streptomyces. In: Ball C (ed) Genetics and breeding of industrial microorganisms. CRC Press, Boca Raton FL, pp 7–42
Hopwood DA, Malpartida F, Kieser HM, Ikeda H, Duncan J, Fujii I, Rudd BAM, Floss HG (1985) Production of hybrid antibiotics by genetic engineering. Nature 314:642–644.
Hoshiko S, Nojiri C, Matsunaga K, Katsumata K, Eriko S, Nagaoka K (1988) Nucleotide sequence of the ribostamycin phosphotransferase gene and of its control region inStreptomyces ribosidificus. Gene 68:285–296
Malpartida F, Hallam SE, Kieser HM, Motamedi H, Hutchinson CR, Butler MJ, Sugden DA (1987) Homology betweenStreptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthesis genes. Nature 325:818–821
Martin P, Jullien E, Courvalin P (1988) Nucleotide sequence ofAcinetobacter baumannii aphA-6 gene: evolutionary and functional implications of sequence homologies with nucleotide-binding proteins, kinases and other aminoglycoside modifying enzymes. Mol Microbiol 25:615–626
Mazodier P, Cossart P, Giraud E, Gasser F (1985) Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes. Nucleic Acids Res 13:195–205
Miller JR, Ingolia TD (1989) Cloning and characterization of beta-lactam biosynthesis genes. Mol Microbiol 3:689–695
Trieu-Cuot P, Courvalin P (1986) Evolution and transfer of aminoglycoside resistance genes under natural conditions. J Antimicrob Chemother [Suppl C] 18:93–102
Trieu-Cuot P, Courvalin P (1987) Origin, evolution and dissemination of antibiotic resistance genes. Micro Science 4:263–266
Vogtli M, Hutter R (1987) Characterisation of the hydroxystreptomycin phosphotransferase gene sph ofStreptomyces glaucescens: nucleotide sequence and promoter analysis. Mol Gen Genet 208:195–203
Walker MS, Walker JB (1970) Streptomycin biosynthesis and metabolism. J Biol Chem 245:6683–6689
Woese CR (1987) Bacterial evolution. Microbiol Rev 51:221–271
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kirby, R. Evolutionary origin of aminoglycoside phosphotransferase resistance genes. J Mol Evol 30, 489–492 (1990). https://doi.org/10.1007/BF02101103
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02101103