Summary
Mapping of sequences specifying the large and small ribosomal RNAs and six polypeptides in the circular 23.7 kbp mitochondrial DNA ofSaccharomyces exiguus has shown that these genes have the same orientation and that a 5 gene cluster is common to this DNA and the 18.9 kbp mtDNA fromTorulopsis glabrata. Included in the preserved region are juxtaposed sequences specifying ATPase subunits 6 and 9 which have the same order and orientation as analogous genes in theEscherichia coli unc operon. The above data, together with knowledge that these two sequences are dispersed in larger yeast mtDNAs, leads us to suggest that larger forms are derived from a smaller ancestral molecule that would have had some resemblance to the mtDNAs ofS. exiguus andT. glabrata.
Similar content being viewed by others
References
Bonitz SG, Coruzzi G, Thalenfeld BE, Tzagoloff A, Macino G (1980) Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrome oxidase. J Biol Chem 255: 11927–11941
Borst P, Grivell LA (1981) One genes intron is another genes exon. Nature 189:439–440
Clark-Walker GD, Azad AA (1980) Hybridizable sequences between cytoplasmic ribosomal RNAs and 3 micron circular DNAs ofSacharomyces cerevisiae andTorulopsis glabrata. Nucleic Acids Res 8:1009–1022
Clark-Walker GD, Sriprakash KS, McArthur CR, Azad AA (1980) Mapping of mitochondrial DNA fromTorulopsis glabrata: location of ribosomal and transfer RNA genes. Curr Genet 1:209–217
Clark-Walker GD, McArthur CR, Daley DJ (1981a) Does mitochondrial DNA length influence the frequence of spontaneous petine mutants in yeasts? Curr Genet 4:7–12
Clark-Walker GD, McArthur CR, Sriprakash KS (1981b) Partial duplication of the large ribosomal RNA sequence in an inverted repeat in circular mitochondrial DNA fromKloeckera africana. J Mol Biol 147:399–416
Clark-Walker GD, Sriprakash KS (1981) Sequence rearrangements between mitochondrial DNAs ofTorulopsis glabrata andKloeckera africana identified by hybridization with six polypeptide encoding regions fromSaccharomyces cerevisiae mitochondrial DNA. J Mol Biol. 151:367–387
Clark-Walker GD, Sriprakash KS (1982) Size diversity and sequence rearrangements in mitochondrial DNAs from yeasts. In: Slonimski PP, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 349–354
Coruzzi G, Bonitz SG, Thalenfeld BE, Tzagoloff A (1981) Assembly of the mitochondrial membrane system. Analysis of the nucleotide sequence and transcripts in the oxi 1 region of yeast mitochondrial DNA. J Biol Chem 256:12780–12787
Darnell JE (1978) Implications of RNA: RNA splicing in evolution of eukaryotic cells. Science 202:1257–1260
Doolittle WF (1978) Genes in pieces; were they ever together? Nature 272:581
Downie JA, Cox GB, Langman L, Ash G, Becker M, Gibson F (1981) Three genes coding for subunits of the membrane sector (Fo) of theEscherichia coli adenosine triphosphatase complex. J Bacteriol 145:200–210
Gay NJ, Walker JE (1981) The ATP operon: nucleotide sequence of the promoter and the genes for the membrane proteins, and the δ subunit ofEscherichia coli ATP synthetase. Nucleic Acids Res 9:3919–3926
Holmes DS, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193–197
Lazowska J, Jacq C, Slonimski PP (1980) Sequence of introns and flanking exons in wild-type and box 3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron. Cell 22:333–348
Li M, Tzagoloff A (1979) Assembly of the mitochondrial membrane system. Sequences of yeast mitochondrial valine and an unusual threonine tRNA gene. Cell 18:47–53
Macino G, Tzagoloff A (1979) Assembly of the mitochondrial membrane system: partial sequence of a mitochondrial ATPase gene inSaccharomyces cerevisiae. Proc Natl Acad Sci USA 76:131–135
Macino G, Tzagoloff A (1980) Assembly of the mitochondrial membrane system: sequence analysis of a yeast mitochondrial ATPase gene containing the oli-2 and oli-4 loci. Cell 20: 507–517
Margulis L (1970) Origin of eukaryotic cells. Yale University Press, New Haven
Nobrega FG, Tzagoloff A (1980) Assembly of the mitochondrial membrane system. DNA sequence and organization of the cytochrome b gene inSaccharomyces cerevisiae D273-10B. J Biol Chem 255:9828–9837
O'Connor RM, McArthur CR, Clark-Walker GD (1976) Respiratory deficient mutants ofTorulopsis glabrata a yeast with circular mitochondrial deoxyribonucleic acid of 6 μm. J Bacteriol 126:959–968
Osinga KA, Evers RF, Van der Laan JC, Tabak HF (1981) A putative precursor for the small ribosomal RNA from mitochondria ofSaccharomyces cerevisiae. Nucleic Acids Res 9:1351–1364
Reanney D (1979) RNA splicing and polynucleotide evolution. Nature 277:598–600
Smith GE, Summers MD (1980) The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethylpaper. Anal Biochem 109:123–129
Thalenfeld BE, Tzagoloff A (1980) Assembly of the mitochondrial membrane system. Sequence of the oxi 2 gene of yeast mitochondrial DNA. J Biol Chem 255:6173–6180
Travassos LRRG, Cury A (1971) Thermophilic enteric yeasts. Ann Rev Microbiol 25:49–74
Van der Walt JP (1970) Genus 16 Saccharomyces. In: Lodder J (ed) The yeasts. North Holland, Amsterdam, pp 555–718
Yarrow D, Nakase T (1978) DNA base composition of species of the genus Saccharomyces. Antonie van Leeuwenhoek, 41:81–88
References
Burke J, RajBhandary UL (1982) Intervening-sequence within the large ribosomal RNA gene ofNeurospora crassa mitochondria. Location of splice junction and identification of a long open reading frame. Cell 32:509–520
Clark-Walker GD (1983) Basis of diversity in mitochondrial DNAs. In: Cavalier-Smith T (ed) The evolution of genome size. John Wiley and Sons, Sussex (in press)
Clark-Walker GD, Sriprakash KS (1983) Map location of transcripts fromTorulopsis glabrata mitochondrial DNA. EMBO J (in press)
Kochel HG, Kuntzel H (1982) Mitochondrial L-rRNA fromAspergillus nidulans: potential secondary structure and evolution. Nucleic Acids Res 10:4795–4801
Waring RB, Davies RW, Scazzochio C, Brown TA (1982) Internal structure of a mitochondrial intron ofAspergillus nidulans. Proc Natl Acad Sci USA 79:6332–6336
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Clark-Walker, G.D., McArthur, C.R. & Sriprakash, K.S. Order and orientation of genic sequences in circular mitochondrial DNA fromSaccharomyces exiguus: Implications for evolution of yeast mtDNAs. J Mol Evol 19, 333–341 (1983). https://doi.org/10.1007/BF02101636
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02101636