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Transport of alkali cations through thin lipid membranes by (222)C10-Cryptand, an ionizable mobile carrier

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Summary

The kinetics of K+ and Na+ transport across the membrane of large unilamellar vesicles (L.U.V.) were compared at two pH's, with two carriers: (222)C 10-cryptand (diaza-1, 10-decyl-5-hexaoxa-4,7,13,16,21,24-bicyclo[8.8.8.]hexacosane) and valinomcyin, i.e. an ionizable macrobicyclic amino polyether and a neutral macrocyclic antibiotic. The rate of cation transport by (222)C10 saturated as cation and carrier concentrations rose. The apparent affinity of (222)C10 for K+ was higher and less pH dependent than that for Na+ but resembled the affinity of valinomycin for K+. The efficiency of (222)C10 transport of K+ decreased as the pH fell and the carrier concentration rose, and was about ten times lower than that of valinomycin. Noncompetitive K+/Na+ transport selectivity of (222)C10 decreased as pH, and cation and carrier concentrations rose, and was lower than that of valinomycin. Transport of alkali cations by (222)C10 and valinomycin was noncooperative. Reaction orders in cationn(S) and carrierm(M) varied with the type of cation and carrier and were almost independent of pH;n(S) andm(M) were not respectively dependent on carrier or cation concentrations. The apparent estimated constants for cation translocation by (222)C10 were higher in the presence of Na+ than of K+ due to higher carrier saturation by K+, and decreased as pH and carrier concentration increased. Equilibrium potential was independent of the nature of carrier and transported cation. Results are discussed in terms of the structural, physicochemical and electrical characteristics of carriers and complexes.

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Authors and Affiliations

  1. Laboratoire de Physiologie Cellulaire and Laboratoire de Chimie des Interactions Moléculaires, Collège de France, 11. Place Marcelin Berthelot, 75231, Paris, Cedex 05, France

    Jean-Marie Lehn

Authors
  1. Madeleine Castaing
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  2. François Morel
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  3. Jean-Marie Lehn
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Castaing, M., Morel, F. & Lehn, JM. Transport of alkali cations through thin lipid membranes by (222)C10-Cryptand, an ionizable mobile carrier. J. Membrain Biol. 89, 251–267 (1986). https://doi.org/10.1007/BF01870668

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  • DOI: https://doi.org/10.1007/BF01870668

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