Summary
Net H+ influx measurements on the giant internodal cells of the alga,Nitella clavata, have been made by transferring the cells to solution of low pH, 4.5 to 4.7, and measuring the pH changes or the amount of acid addition required to maintain the low pH. The latter results are in very good agreement with those of Kitasato (1968) and thus support his hypothesis that theNitella membrane is very permeable to H+. Other results indicate or suggest that the internal pH is changed only slightly under these conditions and that the membrane may have a rather large capacity to adsorb H+ on the external surface.
Similar content being viewed by others
References
Barr, C. E. 1965. Na and K Fluxes inNitella clavata.J. Gen. Physiol. 49:181.
Costerton, J. W. F., MacRobbie, E. A. C. 1970. Ultrastructure ofNitella translucens in relation to ion transport.J. Exp. Bot. 21:535.
Dainty, J., Hope, A. B., Denby, C. 1960. Ionic relations of cells ofChara australis. II. The indifusible anions of the cell wall.Aust. J. Biol. Sci. 13:267.
Goldman, D. 1943. Potential, impedance, and rectification in membranes.J. Gen. Physiol. 27:36.
Hirakawa, S., Yoshimura, H. 1964. Measurements of the intracellular pH in a single cell ofNitella flexilis by means of micro-glass pH electrodes.Jap. J. Physiol. 14:45.
Hogg, J., Williams, E. J., Johnston, R. J. 1969. A simplified method for measuring membrane resistances inNitella translucens.Biochem. Biophys. Acta 150:518.
Hope, A. B. 1965. Ionic relations of cells ofChara australis. X. Effects of bicarbonate ions on electrical properties.Aust. J. Biol. Sci. 18:789.
Kitasato, H. 1968. The influence of H+ on the membrane potential and ion fluxes ofNitella.J. Gen. Physiol. 52:60.
Lannoye, R. J., Tarr, S. E., Dainty, J. 1970. The effects of pH on the ionic and electrical properties of the internodal cells ofChara australis.J. Exp. Bot. 21:543.
MacRobbie, E. A. C. 1970. The active transport of ions in plant cells.Quart. Rev. Biophys. 3:251.
Mailman, D. S., Mullins, L. J. 1966. The electrical measurement of chloride fluxes inNitella clavata.Ausi. J. Biol. Sci. 19:385.
Nishizaki, Y. 1968. Light-induced changes of bioelectric potential inChara.Plant Cell Physiol. 9:377.
Smith, F. A. 1970. The mechanism of chloride transport inCharacean cells.New Phytol. 69:903.
Spanswick, R. M. 1970. Electrophysiological techniques and the magnitudes of membrane potentials and resistances ofNitella translucens.J. Exp. Bot. 21:617.
Spear, D. G., Barr, J. K., Barr, C. E. 1969. Localization of hydrogen ion and chloride ion fluxes inNitella.J. Gen. Physiol. 54:394.
Walker, N. A., Hope, A. B. 1969. Membrane fluxes and electrical conductance inCharacean cells.Aust. J. Biol. Sci. 22:1179.
Williams, E. J., Johnston, R. J., Dainty, J. 1964. The electrical resistance and capacitance of the membranes ofNitella translucens.J. Exp. Bot. 15:1.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rent, R.K., Johnson, R.A. & Barr, C.E. Net H+ influx inNitella clavata . J. Membrain Biol. 7, 231–244 (1972). https://doi.org/10.1007/BF01867917
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01867917