Skip to main content
SpringerLink
Log in

Interaction of furosemide with lipid membranes

  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Summary

The interaction of furosemide with different phospholipids was investigated. Its influence on the lipid structure was inferred from its effect on the phase transition properties of lipids and on the conductance of planar bilayer membranes. The thermotropic properties of dipalmitoyl phosphatidylcholine, phosphatidylethanolamine (natural), dipalmitoyl phosphatidylethanolamine, brain sphingomyelin, brain cerebrosides and phosphatidylserine in the presence and absence of furosemide were investigated by differential scanning calorimetry,. The modifying effect of furosemide seems to be strongest on phosphatidylethanolamine (natural) and sphingomyelin bilayers. The propensity of furosemide to decrease the electrical resistance of planar lipid membranes was also studied and it is shown that the drug facilitates the transport of ions. Partition coefficients of furosemide between lipid bilayers and water were measured.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DSC:

differential scanning calorimetry

PLM:

planar lipid membranes

DPPC:

dipalmitoyl phosphatidylcholine

DMPC:

dimyristoyl phosphatidylcholine

PE:

phosphatidyl ethanol

References

  • Allen, R.C. 1983. Sulfonamide diuretics.In: Diuretics. E.J. Cragoe, editor. pp. 49–200. John Wiley, New York

    Google Scholar 

  • Bach, D. 1983. Calorimetric studies of model and natural membranes.In: Biomembrane Structure and Function. D. Chapman, editor. p. 1. MacMillan, London

    Google Scholar 

  • Barenholz, Y., Suurkuusk, J., Mountacastle, D., Thompson, T.E., Biltonen, R.L. 1976. A calorimetric study of the thermotropic behavior of aqueous dispersions of natural and synthetic sphingomyelins.Biochemistry 15:2441–2447

    Google Scholar 

  • Bruggeman, E.P., Melchior, D.L. 1983. Alterations in the organization of phosphatidylcholine/cholesterol bilayers by tetrahydrocanabinol.J. Biol. Chem. 258:8298–8303

    Google Scholar 

  • Brugnara, C., Canessa, M., Cusi, D., Tosteson, D.C., 1986. Furosemide-sensitive Na and K fluxes in human red cells.J. Gen. Physiol. 87:91–112

    Google Scholar 

  • Calhoun, W.I., Shipley, G.G. 1979. Fatty, acid composition and thermal behavior of natural sphingomyelins.Biochim. Biophys. Acta 555:436–441

    Google Scholar 

  • Canessa, M., Brugnara, C., Cusi, D., Tosteson, D.C. 1986. Modes of operation and variable stoichiometry of the furosemide-sensitive Na and K fluxes in human red cells.J. Gen. Physiol. 87:113–142

    Google Scholar 

  • Carmel, G., Rodrigue, F., Carriere, S., Le Grimellec, C. 1985. Composition and physical properties of lipids from plasma membranes of dog kidneys.Biochim. Biophys. Acta 818:149–157

    Google Scholar 

  • Janiak, M.J., Small, D.M., Shipley, G.G. 1976. Nature of the thermal pretransition of synthetic phospholipids: Dimyristoyl and dipalmitoyl-lecithin.Biochemistry 15:4575–4580

    Google Scholar 

  • Montal, M., Mueller, P. 1972. Formation of biomolecular membranes from lipid monolayers and a study of their electrical properties.Proc. Natl. Acad. Sci. USA 69:3561–3566

    Google Scholar 

  • O'Leary, T.J., Ross, P.D., Levin, J.W. 1984. Effects of anesthetics and nonanesthetic steroids on dipalmitoylphosphatidyl-choline liposomes. A calorimetric and Raman spectroscopic investigations.Biochemistry 23:4636–4641

    Google Scholar 

  • Orita, Y., Ando, A., Takamitsu, Y., Abe, M. 1972. The pK of furosemide and its metal-chelating activity.Chem. Abstr. 77:346

    Google Scholar 

  • Rand, R.P., Chapman, D., Larsson, K. 1975. Tilted hydrocarbon chains of dipalmitoyl lecithin became perpendicular to the bilayer before melting.Biophys. J. 15:1117–1124

    Google Scholar 

  • Shipley, G.G., Avecilla, L.S., Small, D.M. 1974. Phase behavior and structure of aqueous dispersions of sphingomyelin.J. Lipid Res. 15:124–131

    Google Scholar 

  • Velazquez, H., Wright, A.S. 1986. Control by drugs of renal potassium handling.Toxicology 26:293–309

    Google Scholar 

  • Vodyanov, V., Vodyanov, I., Murphy, R.B. 1985. Furosemide blocks the apomorphine-elicited Cl-channel activity of rat striatal dopamine receptors functionally reconstituted into bimolecular lipid membrane.Neurosci. Lett. 62:103–106

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Membrane Research, The Weizmann Institute of Science, 76100, Rehovot, Israel

    Diana Bach, Chana Vinkler, Israel R. Miller & S. Roy Caplan

Authors
  1. Diana Bach
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chana Vinkler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Israel R. Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Roy Caplan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bach, D., Vinkler, C., Miller, I.R. et al. Interaction of furosemide with lipid membranes. J. Membrain Biol. 101, 103–111 (1988). https://doi.org/10.1007/BF01872825

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01872825

Key Words

Search

Navigation