Summary
A new genetic mutant in mice,Hyp, has been proposed as a model for the human disease X-linked hypophosphatemia (the most common form of vitamin D-resistant rickets). The gene is X-linked, dominant, and produces reduced renal tubular reabsorption of phosphate, hypophosphatemia, and dwarfism. Our goal was to evaluate the skeletal changes histologically and to measure chemically the prominant blood and bone minerals to judge the suitability of this mutant as a model for the human disease. Thirteen-week-old hemizygousHyp male mice were compared with their normal littermate controls. TheHyp mice were hypocalcemic, hypophosphatemic, hypermagnesemic, and had elevated plasma alkaline phosphatase. The femur ash weighed less than half the normal ash weight but had a normal Ca:P ratio. The ash composition was high in %Na and K but low in %Mg. The mandibular incisor ash was also low in %Mg. Histologically the femur showed wide osteoid borders and wide epiphyseal plate. Microradiography revealed reduced bone density and enlarged osteocyte lacunae. Skeletal muscle samples, although smaller in theHyp mice, showed no striking alternations in inorganic or total phosphate content, dry weight (as % wet weight), or extracellular fluid space. TheHyp gene in mice seems to produce a condition similar to that of X-linked hypophosphatemia in humans.
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Dent, C.E., Round, J.M., Stamp, T.C.B.: Treatment of sex-linked hypophosphatemic rickets (SLHR). In B. Frame, A.M. Parfitt, H. Duncan (eds.): Clincial aspects of Metabolic Bone Disease, pp. 427–432. Excerpta Medica, Amsterdam, 1973
Williams, T.F., Winters, R.W.: Familial (hereditary) vitamin D-resistant rickets with hypophosphatemia. In J.B. Stanbury, J.B. Wyngaarden, D.S. Fredrickson (eds.): The Metabolic Basis of Inherited Disease, pp. 1465–1485. McGraw-Hill Book Co., New York, 1972
Eicher, E.M., Southard, J.L., Scriver, C.R., Glorieux, F.H.: Hypophosphatemia: mouse model for human familial hypophosphatemic (vitamin D-resistant) rickets, Proc. Natl. Acad. Sci. USA73:4667–4671, 1976
O'Doherty, P.J.A., DeLuca, H.F., Eicher, E.M.: Lack of effect of vitamin D and its metabolites on intestinal phosphate transport in familial hypophosphatemia of mice, Endocrinology101:1325–1330, 1977
Meyer, R.A., Jr., Meyer, M.H.: Altered bone mineral content and composition in the X-linked hypophosphatemic (Hyp) mouse, Fed. Proc.37:436, 1978
Meyer, M.H., Meyer, R.A., Jr.: Unsuitability of porcelain crucibles for dry ashing of biological tissues for calcium analysis, Clin. Chem.22:1396–1398, 1976
Analytical Methods for Atomic Absorption Spectrophotometry. Perkin-Elmer Corp., Norwalk, Conn., 1973
Chen, P.S., Jr., Toribara, T.Y., Warner, H.: Microdetermination of phosphorus, Anal. Chem.28:1756–1758, 1956
Meyer, R.A., Jr., Meyer, M.H.: Phosphate mobilization from striated muscle following parathyroid hormone administration to thyroparathyroidectomized rats, Experientia33:278–279, 1977
Sarkar, B.C.R., Chauhan, U.P.S.: A new method for determining micro quantities of calcium in biological materials, Anal. Biochem.20:155–166, 1967
Cotlove, E., Trantham, H.V., Bowman, R.L.: An instrument and method for automatic, rapid, accurate and sensitive titration of chloride in biologic samples, J. Lab. Clin. Med.51:461–468, 1958
Jowsey, J., Kelly, P.J., Riggs, B.L., Bianco, A.J., Scholz, D.A., Gershon-Cohen, J.: Quantitative microradiographic studies of normal and osteoporotic bone, J. Bone Joint Surg. [Am]47-A:785–806, 1965
Robb, R.A., Jowsey, J.: Quantitative measurement of fractional bone volume using digital scanning videodensitometry, Calcif. Tissue Res.25:265–271, 1978
Jowsey, J: Metabolic Diseases of Bone, pp. 198–199, W.B. Saunders Co., Philadelphia, 1977
Harrison, J.E., Cumming, W.A., Fornasier, V., Fraser, D., Saunders Co., Phildelphia, 1977
Harrison, J.E., Bumming, W.A., Fornasier, V., Fraser, D., Kooh, S.W., McNeill, K.G.: Increased bone mineral content in young adults with familial hypophosphatemic vitamin D refractory rickets, Metabolism25:33–40, 1976
Dent, C.E., Stamp, T.C.B.: Vitamin D, rickets, and osteomalacia. In L.V. Avioli, S.M. Krane (eds.): Metabolic Bone Disease, pp. 237–305. Academic Press, New York, 1977
Cowgill, L., Goldfarb, S., Goldberg, M., Slatopolsky, E., Agus, Z.S.: Nature of the renal defect in familial hypophosphatemic rickets (FHR), Clin. Res.25:505A, 1977
Giasson, S.D., Brunette, M.G., Danan, G., Vigneault, N., Carriere, S.: Micropuncture study of renal phosphorus transport in hypophosphatemic vitamin D resistant rickets mice, Pfluegers Arch.371:33–38, 1977
Scriver, C.R., Glorieux, F.H., Reade, T.M., Tenenhouse, H.S.: X-linked hypophosphatemia and autosomal recessive vitamin D dependency: Models for the resolution of vitamin D refractory rickets. In H. Bickel, J. Stern (eds.): Inborn Errors of Calcium and Bone Metabolism, pp. 150–178. University Park Press, Baltimore, 1976
Meyer, R.A., Jr., Gray, R.W., Meyer, M.H.: Paradoxical normal levels of vitamin D metabolites in the plasma of the X-linked hypophosphatemic mouse. In: Program and Abstracts, 60th Annual Meeting, The Endocrine Society, p. 486, 1978
Haussler, M., Hughes, M., Baylink, D., Littledike, E.T., Cork, D., Pitt, M.: Influence of phosphate depletion on the biosynthesis and circulating level of 1α, 25-dihydroxyvitamin D. In S.G. Massry, E. Ritz, (eds.): Phosphate Metabolism, pp. 233–250. Plenum Press, New York, 1977
Anast, C.S.: Magnesium studies in relation to vitamin D-resistant rickets, Pediatrics40:425–435, 1967
Neuman, W.F.: The milieu interieur of bone: Claude Bernard revisited, Fed. Proc.28:1846–1850, 1969
Talmage, R.V.: Calcium homeostasis-calcium transport-parathyroid action. The effects of parathyroid hormone on the movement of calcium between bone and fluid, Clin. Orthop.67:210–224, 1969
Ramp, W.K., Thomas, J.R., Nifong, P.D.: Magnesium and the mineral metabolism of chick embryo tibiae in organ culture, Calcif. Tissue Res.24:93–98, 1977
Bixler, D.: Heritable disorders affecting dentin. In R.E. Stewart, G.H. Prescott (eds.): Oral Facial Genetics, pp 249–253. C.V. Mosby Company, St. Louis, 1976
Iorio, R.J., Bell, W.A., Meyer, M.H., Meyer, R.A., Jr.: Histologic evidence of calcification abnormalities in teeth and alveolar bone of mice with X-linked dominant hypophosphatemia (VDRR), Ann. Dent. (in press)
Iorio, R.J., Bell, W.A., Meyer, M.H., Meyer, R.A., Jr.: Radiographic evidence of craniofacial and dental abnormalities in the X-linked hypophosphatemic mouse, Ann. Dent. (in press) 1978
Sciver, C.R., Reade, T.M., Deluca, H.F. Hamstra, A.J.: Serum 1,25-dihydroxyvitamin D levels in normal subjects and in patients with hereditary rickets or bone disease, N. Engl. J. Med.299:976–979, 1978
Tenenhouse, H.S., Scriver, C.R., McInnes, R.R., Glorieux, F.H.: Renal handling of phosphatein vivo andin vitro by the X-linked hypophosphatemic male mouse: Evidence for a defect in the brush border membrane, Kidney Internat.14:236–244, 1978
Tenenhouse, H.S., Scriver, C.R.: The defect in transcellular transport of phosphate in the nephron is located in brushborder membranes in X-linked hypophosphatemia (Hyp mouse model), Can. J. Biochem.56:640–646, 1978
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Meyer, R.A., Jowsey, J. & Meyer, M.H. Osteomalacia and altered magnesium metabolism in the X-linked hypophosphatemic mouse. Calcif Tissue Int 27, 19–26 (1979). https://doi.org/10.1007/BF02441156
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DOI: https://doi.org/10.1007/BF02441156