Abstract
Laser effects on human chromosomes have been studied with an image cytometer using an argon ion laser emitted beam at 488 nm, with a 50 mW power. The smallest laser spot was of 0.2Μm. Aiming of the laser beam was controlled by a computer. Irradiated and non-irradiated chromosomal fragments were analysed by measuring the fluorescence intensity of propidium iodide and by fluorescence in situ hybridization (FISH) with fluorescein biotinylated Alu polymerase chain reaction products. No propidium iodide staining or FISH could be observed on irradiated chromosome fragments indicating total elimination of DNA by the laser beam. The non-irradiated fragments of chromosome showed Alu hybridization similar to control metaphase spreads, suggesting that the DNA structure remained intact. This methodological approach could be used to carry out precise and rapid microdissection of chromosomes.
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Métézeau, P., Tachdjian, G., Kiefer, H. et al. Analysis of laser effects on chromosomal structure by fluorescence in situ hybridization and image cytometry: Methodological approach for precise microdissection. Laser Med Sci 10, 261–266 (1995). https://doi.org/10.1007/BF02133618
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DOI: https://doi.org/10.1007/BF02133618