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The dopaminergic innervation of the goldfish pituitary

An immunocytochemical study at the electron-microscope level using antibodies against dopamine

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Summary

The dopaminergic innervation of the goldfish pituitary gland was studied by immunocytochemistry at the electron-microscope level using highly specific antibodies against dopamine coupled to bovine serum albumin with glutaraldehyde. A satisfactory preservation of the tissue was achieved after immersion in 5% glutaraldehyde in phosphate buffer containing sodium metabisulfite to prevent oxidation of the endogenous dopamine. The immunocyto-chemical procedure was performed on Vibratome sections using the preembedding method. Immunoreactivity was restricted to part of the neurosecretory type-B fibers (diameter of the secretory vesicles lower than 100 nm) in which it was found to occupy the whole cytoplasm. Labeled fibers were observed within the neurohypophysis in the different parts of the gland and in the adenohypophyseal tissue where immunoreactive profiles were detected in close apposition to the different cell types. These data are in agreement with previous results obtained by means of radioautography and further support a role for dopamine in the neuroendocrine regulation of pituitary functions in teleosts.

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Authors and Affiliations

  1. Laboratoire de Physiologie des Interactions Cellulaires, UA CNRS 339, Talence, France

    Olivier Kah, Pierrette Dubourg, Brigitte Onteniente & André Calas

  2. Laboratoire de Neuroimmunologie, Institut de Biologie Cellulaire et de Neurochimie du CNRS, Bordeaux, France

    Michel Geffard

Authors
  1. Olivier Kah
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  2. Pierrette Dubourg
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  3. Brigitte Onteniente
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  4. Michel Geffard
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  5. André Calas
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Kah, O., Dubourg, P., Onteniente, B. et al. The dopaminergic innervation of the goldfish pituitary. Cell Tissue Res. 244, 577–582 (1986). https://doi.org/10.1007/BF00212536

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