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Localization of gonadotrophic hormones in the pituitary gland of the California leaf-nosed bat (Macrotus californieus)

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Summary

Luteinizing hormone (LH) and follicle-stimulating hormone (FSH) cells were immunostained in the anterior pituitary lobe of the California leaf-nosed bat with antisera against the specific beta (β) subunits of rat LH and rat FSH employing the unlabeled immunoperoxidase technique. Selective immunochemical staining by both antisera was localized primarily in the same cell type. However, some cells consistently reacted solely with antisera to either LH-β or FSH-β. Whereas the immunoreactive LH-β cells, in general, stained more intensely than FSH-β cells they were usually less numerous. These cells were alcian-blue and PAS-positive, staining pink to purple. The LH/FSH cells were pleomorphic with eccentric nuclei, often with cytoplasmic extensions. They were found throughout the pars distalis.

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Authors and Affiliations

  1. Department of Anatomy, The University of Arizona, Health Sciences Center, Tucson, Arizona, USA

    Bruce A. Richardson

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  1. Bruce A. Richardson
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Additional information

Submitted to the Graduate College at The University of Arizona, Tucson, Arizona in partial fulfillment of the requirements for the Ph.D. degree. This work was supported in part by a Grant from the University of Arizona Graduate College to the author, USPHS GRS Grant RR-05675 and Grant No. 302867 from The University of Arizona Alumni Association and University Foundation to P.H. Krutzsch

The author would like to extend his appreciation to Dr. P.H. Krutzsch for his counsel and assistance throughout the course of this study. The author also wishes to thank Dr. A.F. Parlow who kindly supplied the antisera and antigens utilized in this study under the auspices of the Hormone Distribution Program of NIAMDD and Dr. L.A. Sternberger for the PAP complex. Dr. R.J. Reiter is acknowledged for his critical review and Nancy Elms for her skillful typing of this manuscript

The author is presently a NIH Postdoctoral Fellow (1 F32ND05900)

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Richardson, B.A. Localization of gonadotrophic hormones in the pituitary gland of the California leaf-nosed bat (Macrotus californieus). Cell Tissue Res. 220, 115–123 (1981). https://doi.org/10.1007/BF00209970

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