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5-Ethyl-2′-deoxyuridine: Absence of effects on the chromosomes of human lymphocytes and fibroblasts in culture

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Summary

In vitro studies have been performed with 5-ethyl-2′-deoxyuridine (EDU) concerning its incorporation into the cell nuclear material and its effects on the chromosome morphology of cultivated human lyphocytes and skin fibroblasts. This compound is presumably incorporated in the DNA without visible chromosome aberrations as is seen with many other pyrimidine analogues. A slight inhibition of the cell growth was noted at high concentration (120μg/ml) of EDU. A similar degree of cell growth inhibition was found with corresponding doses of deoxythymidine.

Zusammenfassung

Es wurden in vitro-Untersuchungen hinsichtlich des Äthyldeoxyuridineinbaus (ÄDU) und dessen Einfluß auf menschliche Lymphocyten- und Fibroblastenchromosomen durchgeführt. Diese Substanz wird in die DNS eingebaut und führt nicht zu sichtbaren Chromosomenaberrationen wie bei einigen anderen Pyrimidinanaloga. Eine leichte Hemmung des Zellwachstums wurde mit 120 μg/ml ÄDU festgestellt. Eine ähnliche Hemmung des Zellwachstums ließ sich auch mit gleicher Deoxythymidinkonzentration nachweisen.

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Authors and Affiliations

  1. Institute of Human Genetics, University of Hamburg, Hamburg, Germany

    S. Singh, I. Willers & H. W. Goedde

  2. Ophthalmology Clinie, University of Hamburg, Hamburg, Germany

    K. K. Gauri

Authors
  1. S. Singh
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  2. I. Willers
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  3. H. W. Goedde
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  4. K. K. Gauri
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Supported by the Deutsche Forschungsgemeinschaft.

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Singh, S., Willers, I., Goedde, H.W. et al. 5-Ethyl-2′-deoxyuridine: Absence of effects on the chromosomes of human lymphocytes and fibroblasts in culture. Hum Genet 24, 135–139 (1974). https://doi.org/10.1007/BF00283770

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  • DOI: https://doi.org/10.1007/BF00283770

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