Summary
Rts1 is a kanamycin-resistance plasmid and multiphenotypically thermosensitive. The detrimental host cell growth at 42° C is expressed only when it exitst autonomously but not in an integrated state. However, a cholate-resistant (plt) mutant of the Hfr with an integrated Rts1 plasmid was found to be thermosensitive like a strain with the same plasmid autonomoulsy. This thermosensitivity depends on the existence of the integrated plasmid. Deletion derivatives of integrated plasmid genome from this Hfr strain were isolated with or without thermal selective growth at 42° C and mapping of the plasmid was attempted by analyzing them. A total of 141 kanamycin-sensitive derivatives were independently isolated and examined for their thermosensitivity (genetic locus: tsg), incompatibility (genetic locus: incT), conjugal fertility (genetic locus: tra), ‘restriction’ of T4 phase (two genetic loci: resA and resB) and for DNase activity (genetic locus: dns). On the basis of characterization of 141 deletion derivatives, they were divided into 15 patterns which would correspond to a linear map integrated into the chromosome: ... resA ... dns ... resB ... tra ... kan ... incT ... tsg ... It is noteworthy that ‘restriction’ of T4D phage is determined by two distinct genes, resA and resB, intervened by dns and that propagation of T4D phage on a strain with a resA + resB - genome failed to produce modified progeny phages.
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Adams, M.H.: Bacteriophages. New York: Interscience Publishers 1959
Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K 12. Bacteriol. Rev. 40, 116–167 (1976)
Coetzee, J.N., Datta, N., Hedges, R.W.: R factors from Proteus rettgeri. J. Gen. Microbiol. 72, 543–552 (1972)
Danbara, H., Yoshikawa, M.: Non-random distribution of transduction termini in transductants from the integrated R plasmid, R100-1. Mol. Gen. Genet. 171, 145–152 (1979)
Freifelder, D., Folkmanis, A., Kirschner, I.: Studies on Escherichia coli sex factors: Evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. J. Bacteriol. 105, 722–727 (1971)
Hirota, Y.: The effect of acridine dyes on mating type factors in E. coli. Proc. Natl. Acad. Sci. USA 46, 57–64 (1960)
Ishihara, M., Kamio, Y., Terawaki, Y.: Cupric ion resistance as a new genetic marker of a temperature sensitive R plasmid, Rts1 in Escherichia coli. Biochem. Biophys. Res. Commun. 82, 81–84 (1978)
Lederberg, J.: Isolation and characterization of biochemical mutants of bacteria. Methods Med. Res. 3, 5–22 (1950)
Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190–206 (1955)
Matsumoto, H., Kamio, Y., Kobayashi, R., Terawaki, Y.: R plasmid, Rts1-mediated production of extracellular deoxyribonuclease in Escherichia coli. J. Bacteriol. 133, 387–389 (1978)
Sasakawa, C., Yoshikawa, M.: Requirements for suppression of a dnaG mutation by an I-type plasmid. J. Bacteriol. 133, 485–491 (1978)
Streifeld, M.M., Hoffmann, E.M., Janklow, H.M.: Evaluation of extracellular deoxyribonuclease activity in Pseudomonas. J. Bacteriol. 84, 77–80 (1962)
Terawaki, Y., Kakizawa, Y., Takayasu, H., Yoshikawa, M.: Temperature sensitivity of cell growth in Escherichia coli associated with the temperature sensitive R (KM) factor. Nature 219, 284–285 (1968)
Terawaki, Y., Takayasu, H., Akiba, T.: Thermosensitive replication of a kanamycin resistance factor. J. Bacteriol. 94, 687–690 (1967)
Yokota, T., Kanamaru, Y., Mori, R., Akiba, T.: Recombination between a thermosensitive kanamycin resistance factor and a nonthermosensitive multiple drug resistance factor. J. Bacteriol. 98, 863–873 (1969)
Yoshida, Y., Takamatsu, N., Yoshikawa, M.: Preferential inhibitory action of sodium cholate on an Escherichia coli strain carrying a plasmid in an integrated state. J. Bacteriol. 133, 406–408 (1978)
Yoshikawa, M.: Thermosensitive cell growth and permeability coded by an Rts1 plasmid and their suppression upon integration into the chromosome in Escherichia coli. Biochem. Biophys. Res. Commun. 86, 119–123 (1979)
Yoshikawa, M., Yoshimoto, H., Sotomura, M., Takamatsu, N., Yoshida, Y.: Plasmid-chromosome interaction in Hfr strains. In: Plasmids-medical and theoretical aspects (Mitsuhashi, S., Rosival, L., Krčméry, V., eds.), pp. 177–184. Prague: Czechoslovak Medical Press; Berlin-Heidelberg-New York: Springer 1977
Yoshimoto, H., Yoshikawa, M.: Chromosome-plasmid interaction in Escherichia coli K-12 carrying a thermosensitive plasmid, Rts1, in autonomous and in integrated states. J. Bacteriol. 124, 661–667 (1975)
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Communicated by D. Goldfarb
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Sasakawa, C., Tsuchiya, T. & Yoshikawa, M. Isolation and characterization of plasmid-deletion derivatives from an Hfr made with Rts1 plasmid carrying a chromosomal mutation, plt . Molec. Gen. Genet. 177, 243–250 (1980). https://doi.org/10.1007/BF00267435
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DOI: https://doi.org/10.1007/BF00267435