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The separation of viable rye microspores from mixed populations and their development in culture

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Summary

A technique is described to isolate rye microspores from anthers precultured on agar and from intact spikes. Highly purified fractions consisting predominantly of viable late uninucleate or early binucleate microspores are obtained by filtration and centrifugation techniques. The preparations form an excellent starting material to attempt to increase the frequency of microspore plantlet formation above that normally obtained by anther culture. Microspores isolated from anthers precultured for five or six days on agar, develop after three weeks culture in a liquid medium into multicellular structures, the largest of which burst through the microspore wall.

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Authors and Affiliations

  1. Projektgruppen Haploide in der Pflanzenzüchtung, Max-Planck-Institut für Pflanzengenetik, Ladenburg-Rosenhof

    Gerhard Wenzel, Franz Hoffmann, Ingo Potrykus & Emrys Thomas

Authors
  1. Gerhard Wenzel
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  2. Franz Hoffmann
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  3. Ingo Potrykus
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  4. Emrys Thomas
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Communicated by G. Melchers

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Wenzel, G., Hoffmann, F., Potrykus, I. et al. The separation of viable rye microspores from mixed populations and their development in culture. Molec. Gen. Genet. 138, 293–297 (1975). https://doi.org/10.1007/BF00264798

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  • DOI: https://doi.org/10.1007/BF00264798

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