Abstract
The effects of growth retardants, 4-hydroxy-5-isopropyl-2-methylphenyltrimethylammonium chloride-1-piperidine carboxylate (AMO-1618 or AMO) and 2-chloroethyltrimethylammonium chloride (CCC), applied with and without gibberellic acid (GA3), on β-[3H]alanine uptake and leakage from pea (Pisum sativum L.) and betacyanin efflux from beetroot (Beta vulgaris L.) tissue were examined. Both compounds decreased the amount of β-[3H]alanine taken up into pea leaf discs, and increased the quantity of radioactive label that subsequently leaked out of this tissue. Efflux of betacyanin from slices of beetroot was also found to be promoted by treatment with CCC or AMO-1618. In no case were these effects reversed by application of GA3. It is concluded that the growth retardants may be altering tissue permeability by an interaction with the cell membranes, and this may account for some of the side effects of the retardants which cannot be explained on the basis of their inhibiting action on gibberellin synthesis.
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Abbreviations
- AMO-1618:
-
4-hydroxy-5-isopropyl-2-methylphenyltrimethylammonium chloride-1-piperidine carboxylate
- CCC:
-
2-chloroethyltrimethylammonium chloride
- GA3 :
-
gibberellic acid
References
Altman, A., Kaur-Sawhney, R., Galston, A.W. (1977) Stabilization of oat leaf protoplasts through polyamine-mediated inhibition of senescence. Plant Physiol. 60, 570–574
Barnes, M.F., Light, E.N., Lang, A. (1969) The action of plant growth retardants on terpenoid biosynthesis. Inhibition of gibberellic acid production in Fusarium moniliforme by CCC and AMO-1618; action of these retardants on sterol biosynthesis. Planta 88, 172–182
Berry, D.R., Smith, H. (1970) The inhibition by high concentrations of (2-chloroethyl)-trimethylammonium chloride (CCC) of chlorophyll and protein synthesis in excised barley leaf sections. Planta 91, 80–86
Crozier, A., Reid, D.M., Reeve, D.R. (1973) Effects of AMO-1618 on growth, morphology, and gibberellin content of Phaseolus coccineus seedlings. J. Exp. Bot. 24, 923–934
Douglas, T.J., Paleg, L.G. (1974) Plant growth retardants as inhibitors of sterol biosynthesis in tobacco seedlings. Plant Physiol. 54, 238–245
Douglas, T.J., Paleg, L.G. (1974) AMO-1618 effects on incorporation of 14C-MVA and 14C-acetate into sterols in Nicotiana and Digitalis seedlings and cell-free preparations from Nicotiana. Phytochemistry 17, 713–718
Kende, H., Ninnemann, H., Lang, A. (1963) Inhibition of gibberellic acid biosynthesis in Fusarium moniliforme by AMO-1618 and CCC. Naturwissenschaften 50, 599–560
Knypl, J.S. (1971) Control of protein and RNA synthesis by AMO-1618 and other growth retardants in cucumber cotyledons. Biochem. Physiol. Pfl. 162, 127–141
Lüttge, U., Higinbotham, N. (1979) Transport in plants. Springer, New York, Heidelberg, Berlin
Naik, B.I., Srivastava, S.K. (1978) Effect of polyamines on tissue permeability. Phytochemistry 17, 1885–1887
Poovaiah, B.W., Leopold, A.C. (1976) Effects of inorganic salts on tissue permeability. Plant Physiol. 58, 182–185
Prochaska, L.J., Gross, E.L. (1977) Evidence for the location of divalent cation binding sites on the chloroplast membrane. J. Membr. Biol. 36, 13–32
Reid, D.M., Crozier, A. (1970) CCC-induced increase of gibberellin levels in pea seedlings. Planta 94, 95–106
Robinson, D.R., West, C.A. (1970) Biosynthesis of cyclic diterpenes in extracts from seedlings of Ricinus communis L. II. Conversion of geranylgeranyl pyrophosphate in diterpene hydrocarbons and partial purification of the cyclization enzymes. Biochemistry 9, 80–89
Satter, R.L., Applewhite, P.B., Kreis, D.J., Galston, A.W. (1973) Rhythmic leaflet movement in Albizzia julibrissin. Effects of electrolytes and temperature alteration. Plant Physiol. 52, 202–207
Shechter, I., West, C.A. (1969) Biosynthesis of gibberellins. IV. Biosynthesis of cyclic diterpenes from trans-geranyl-geranyl pyrophosphate. J. Biol. Chem. 244, 3200–3209
Shewry, P.R., Pinfield, N.J., Stobart, A.K. (1971) The effect of 2,4-dichlorophenoxyacetic acid and (2-chloroethyl)-trimethylammonium chloride on chlorophyll synthesis in barley leaves. Planta 101, 352–359
van Bragt, J. (1969) The effect of CCC on growth and gibberellin content of tomato plants. Neth. J. Agric. Sci. 17, 183–188
Van Thoai, N. (1965) Nitrogenous bases. In: Comprehensive Biochemistry Volume 6; Lipids Amino Acids and Related Compounds, Florkin, M., Stotz, E.H., eds. Amsterdam: Elsevier Publishing Co.
von Hippel, P.M., Schleich, T. (1969) The effects of neutral salts on the structure and confirmational stability of macromolecules in solution. In: Structure and Stability of Biological Macromolecules, Timashef, S.N., Fasman, G.D., eds. Marcel Decker, New York
von Hippel, P.H., Wong, K.Y. (1964) Neutral salts: the generality of their effects on the stability of macromolecular conformations. Science 145, 577–580
Wünsche, U. (1969) Growth retarding and stimulating effects of CCC on Antirrhinum majus L. Planta 85, 108–110
Zeevaart, J.A.D. (1966) Reduction in the gibberellin content of Pharbitis seeds by CCC and after-effects in the progeny. Plant Physiol. 41, 856–861
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Fabijan, D.M., Dhindsa, P.P. & Reid, D.M. Effects of two growth retardants on tissue permeability in Pisum sativum and Beta vulgaris . Planta 152, 481–486 (1981). https://doi.org/10.1007/BF00380817
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DOI: https://doi.org/10.1007/BF00380817