Abstract
We studied the fine structure of mitotic chromosome cores (scaffolds) in spermatogonia of Trilophidia annulata by the squash method, the silver staining technique, and light and electron microscopy. The chromosome core seemed to be helically coiled when viewed in the light microscope. Electron microscopic in situ observations on the squash preparations transferred from slides to grids indicated that the core was basically a compact network of fibres, rather than a simple coiled structure. In the core, there were two longitudinal main fibres, which were relatively thick and twined about one another. Each of the main fibres consisted of thinner fibres. The twined fibres composed the network structure of the core. Based on these observations, we discuss the morphological features of the core.
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by T.C. Hsu
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Zhao, J., Hao, S. & Xing, M. The fine structure of the mitotic chromosome core (scaffold) of Trilophidia annulata . Chromosoma 100, 323–329 (1991). https://doi.org/10.1007/BF00360531
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DOI: https://doi.org/10.1007/BF00360531